Limit of detection of PCR/RFLP analysis of cytochrome oxidase II for the identification of genetic groups of Trypanosoma cruzi and Trypanosoma rangeli in biological material from vertebrate hosts

Trypanosoma rangeli is non pathogenic for humans but of important medical and epidemiological interest because it shares vertebrate hosts, insect vectors, reservoirs and geographic areas with T. cruzi, the etiological agent of Chagas disease. Therefore, in this work, we set up two PCR reactions, TcH2AF/R and TrFR2, to distinguish T. cruzi from T. rangeli in mixed infections of vectors based on amplification of the histone H2A/SIRE and the small nucleolar RNA Cl1 genes, respectively. Both PCRs were able to appropriately detect all T. cruzi or T. rangeli experimentally infected-triatomines, as well as the S35/S36 PCR which amplifies the variable region of minicircle kDNA of T. cruzi. In mixed infections, whereas T. cruzi DNA was amplified in 100% of samples with TcH2AF/R and S35/S36 PCRs, T. rangeli was detected in 71% with TrF/R2 and in 6% with S35/S36. In a group of Rhodnius colombiensis collected from Coyaima (Colombia), T. cruzi was identified in 100% with both PCRs and T. rangeli in 14% with TrF/R2 and 10% with S35/S36 PCR. These results show that TcH2AF/R and TrF/R2 PCRs which are capable of recognizing all T. cruzi and T. rangeli strains and lineages could be useful for diagnosis as well as for epidemiological field studies of T. cruzi and T. rangeli vector infections.

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Protective effect of Trypanosoma rangeli against infections with a highly virulent strain of Trypanosoma cruzi

Tropical Medicine & International Health ◽

10.1046/j.1365-3156.1997.d01-297.x ◽

1997 ◽

Vol 2 (5) ◽

pp. 482-487 ◽

Cited By ~ 1

Author(s):

Claudio Zuniga ◽

Teresa Palau ◽

Pilar Penin ◽

Carlos Gamallo ◽

Jose Antonio de Diego

Keyword(s):

Trypanosoma Cruzi ◽

Protective Effect ◽

Virulent Strain ◽

Trypanosoma Rangeli ◽

Highly Virulent

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Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽

10.1055/s-0028-1084538 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

P Rojsanga ◽

W Gritsanapan ◽

W Leelamanit ◽

S Sukrong

Keyword(s):

Medicinal Plant ◽

Rflp Analysis ◽

Molecular Authentication ◽

Pcr Rflp

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Genetic Variation in Yellowfin Tuna Thunnus Albacares (Bonnaterre, 1788) Along Indian Coast Using Pcr-Rflp Analysis of Mitochondrial Dna D-Loop Region

International Journal of Scientific Research ◽

10.15373/22778179/jan2014/8 ◽

2012 ◽

Vol 3 (1) ◽

pp. 25-30

Author(s):

Swaraj Priyaranjan Kunal ◽

◽

Girish Kumar Girish Kumar ◽

Maria Rosalia Menezes

Keyword(s):

Mitochondrial Dna ◽

Genetic Variation ◽

Rflp Analysis ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Indian Coast ◽

Loop Region ◽

Pcr Rflp ◽

D Loop

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PCR-RFLP Analysis Of Insulin-Like Growth Factor-I Gene In Bali Cattle

Jurnal Ilmu Peternakan Dan Veteriner ◽

10.30862/jipv.v5i1.756 ◽

2018 ◽

Vol 5 (1) ◽

Author(s):

Muhamad Affan Mu’in

Keyword(s):

Growth Factor ◽

Rflp Analysis ◽

Insulin Like Growth Factor ◽

Factor I ◽

Pcr Rflp ◽

Growth Factor I ◽

Bali Cattle ◽

I Gene

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Genetic and Biochemical Markers of Ovarian Function and Their Impact on Women Reproductive Status

Revista de Chimie ◽

10.37358/rc.20.10.8365 ◽

2020 ◽

Vol 71 (10) ◽

pp. 212-217

Author(s):

Adina-Elena Tanase ◽

Roxana Popescu ◽

Mircea Onofriescu ◽

Roxana Daniela Matasariu

Keyword(s):

Ovarian Function ◽

Rflp Analysis ◽

Fsh Receptor ◽

Gene Encoding ◽

Female Population ◽

Fshr Gene ◽

Pcr Rflp ◽

Regulation Mechanisms ◽

American Society ◽

Dependent Mechanism

Endometriosis is a disease very common nowadays affecting 1-2% of the female population, by estrogen-dependent mechanism. The identification of mutations in the gene encoding for the FSH receptor (FSHR) has been reported since 1995. Physiology teaches us that follicle-stimulating hormone (FSH) is a hormone that is vital in the steroidogenesis regulation mechanisms, while FSH receptor (FSHR) activation helps to promote folliculogenesis and estrogensynthesis. Therefore, studies to show if there are any correlations between endometriosis and FSHR are acquired. Genotyping of FSHR gene polymorphisms were performed using PCR - Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. We analysed a total of 78 patients, 44 infertile patients with endometriosis and 34 controls (non-infertile, pregnant patients). The endometriosis group included women with diagnosis of endo-metriosis confirmed by laparoscopy and /or laparotomy and histological evidence of disease with the endometriosis staging according to American Society for Reproductive Medicine (ASRM). Corroborated with the severity of endometriosis, A919G and A2039G tests found that 71.4% of the M (GG) results were associated with primary infertility, not statistically significant (p=0.994) and 42.9% of the total M results had moderate or severe forms of endometriosis (p = 0.185). The genetic involvement in different pathologies such as endometriosis, has yet to be understood, but knowing more about its mechanism, will help physician target the disease at a more profound level.

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