scholarly journals Molecular phylogeny of the Anopheles hyrcanus group (Diptera: Culicidae) based on rDNA–ITS2 and mtDNA–COII

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Canglin Zhang ◽  
Rui Yang ◽  
Linbo Wu ◽  
Chunhai Luo ◽  
Xiaofang Guo ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Molecular Identification ◽  
Malaria Vector ◽  
Related Species ◽  
Genetic Divergence ◽  
Morphological Characteristics ◽  
Population Expansion ◽  
Closely Related Species ◽  
Rdna Its2 ◽  
Interspecific Divergence

Abstract Background The Anopheles hyrcanus group, which includes 25 species, is widely distributed in the Oriental and Palaearctic regions. Given the difficulty in identifying cryptic or sibling species based on their morphological characteristics, molecular identification is regarded as an important complementary approach to traditional morphological taxonomy. The aim of this study was to reconstruct the phylogeny of the Hyrcanus group using DNA barcoding markers in order to determine the phylogenetic correlations of closely related taxa and to compare these markers in terms of identification efficiency and genetic divergence among species. Methods Based on data extracted from the GenBank database and data from the present study, we used 399 rDNA–ITS2 sequences of 19 species and 392 mtDNA–COII sequences of 14 species to reconstruct the molecular phylogeny of the Hyrcanus group across its worldwide range. We also compared the performance of rDNA–ITS2 against that of mtDNA–COII to assess the genetic divergence of closely related species within the Hyrcanus group. Results Average interspecific divergence for the rDNA–ITS2 sequence (0.376) was 125-fold higher than the average intraspecies divergence (0.003), and average interspecific divergence for the mtDNA–COII sequence (0.055) was eightfold higher than the average intraspecies divergence (0.007). The barcoding gap ranged from 0.015 to 0.073 for rDNA–ITS2, and from 0.017 to 0.025 for mtDNA–COII. Two sets of closely related species, namely, Anophels lesteri and An. paraliae, and An. sinensis, An. belenrae and An. kleini, were resolved by rDNA–ITS2. In contrast, the relationship of An. sinensis/An. belenrae/An. kleini was poorly defined in the COII tree. The neutrality test and mismatch distribution revealed that An. peditaeniatus, An. hyrcanus, An. sinensis and An. lesteri were likely to undergo hitchhiking or population expansion in accordance with both markers. In addition, the population of an important vivax malaria vector, An. sinensis, has experienced an expansion after a bottleneck in northern and southern Laos. Conclusions The topology of the Hyrcanus group rDNA–ITS2 and mtDNA–COII trees conformed to the morphology-based taxonomy for species classification rather than for that for subgroup division. rDNA–ITS2 is considered to be a more reliable diagnostic tool than mtDNA–COII in terms of investigating the phylogenetic correlation between closely related mosquito species in the Hyrcanus group. Moreover, the population expansion of an important vivax malaria vector, An. sinensis, has underlined a potential risk of malaria transmission in northern and southern Laos. This study contributes to the molecular identification of the Anopheles hyrcanus group in vector surveillance. Graphical abstract

scholarly journals Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Congzhao Fan ◽  
Xiaojin Li ◽  
Jun Zhu ◽  
Jingyuan Song ◽  
Hui Yao
Keyword(s):  
Medicinal Plant ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
Its2 Sequence ◽  
Sequence Length ◽  
Local Alignment ◽  
Accurate Identification ◽  
Distance Method ◽  
Closely Related Species

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

scholarly journals SPERGULARIA IRAQENSIS (CARYOPHYLLACEAE), A NEW SPECIES FROM IRAQ

2021 ◽  
Vol 16 (3) ◽  
pp. 291-299
Author(s):  
Ali Haloob ◽  
Ali H. E. Al-Musawi ◽  
Harb Adeel
Keyword(s):  
New Species ◽  
Related Species ◽  
Morphological Characteristics ◽  
Identification Key ◽  
Similar Species ◽  
Closely Related Species ◽  
A New Species

Spergularia iraqensis sp. nov. is described as a new species from Iraq. This species has been collected from Diyala Province in the central east of Iraq; it is closely related to Spergularia rubra (L.) J. Presl & C. Presl, 1819 and Spergularia bocconei (Scheele) Graebn., 1919. The distinguishing of the morphological characteristics of the new species alongside the two similar species are discussed with photographs, and an identification key is given for Spergularia iraqensis and other closely related species.

scholarly journals The larvae of Chinese Hydropsychidae (Insecta: Trichoptera), Part II: Potamyia chinensis and Cheumatopsyche trifascia

Zootaxa ◽  
2021 ◽  
Vol 4926 (4) ◽  
pp. 547-558
Author(s):  
AO ZHANG ◽  
XIN ZHOU
Keyword(s):  
Dna Markers ◽  
Related Species ◽  
Morphological Characteristics ◽  
Species Boundaries ◽  
Closely Related Species ◽  
Molecular Analyses ◽  
Mitochondrial Coi ◽  
Associated Species

The larvae of Chinese caddisflies Potamyia chinensis and Cheumatopsyche trifascia were successfully associated with identifiable adults using independent DNA markers, mitochondrial COI barcodes and nuclear ribosomal 28S D2 genes. A total of 49 specimens collected in China were employed in the molecular analyses. The two markers were congruent on species boundaries for 11 distinctive haplogroups, while D2 failed in differentiating two closely related species. A brief summary for larval studies of both genera is given, followed by an introduction to the generic morphological characteristics, and detailed morphological descriptions and illustrations for the two successfully associated species. The larva of P. chinensis is re-described here based on Chinese materials, following the previous larval description for P. echigoensis, which was recently synonymized with P. chinensis.

scholarly journals New records of two Lutjanus species (Teleostei: Perciformes: Lutjanidae) with re-description of six lutjanids from Saint Martin’s Island of the Bay of Bengal, Bangladesh

2018 ◽  
Vol 46 (2) ◽  
pp. 239-253 ◽  
Author(s):  
Shilpi Saha ◽  
Sanjay Chakraborty ◽  
Subrina Sehrin ◽  
Abdullah Al Masud ◽  
Kazi Ahsan Habib ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bay Of Bengal ◽  
Related Species ◽  
Genetic Divergence ◽  
New Records ◽  
Morphological Differentiation ◽  
Morphological Characters ◽  
Mitochondrial Cytochrome ◽  
Closely Related Species ◽  
Mitochondrial Cytochrome Oxidase

Investigations on Snapper fish of the genus Lutjanus were conducted from October 2015 to April 2016 in the coral ecosystem of St. Martin’s Island, Bangladesh. Twenty one individuals of 8 species of Lutjanus were collected from local fishermen. Both morphological characters and DNA barcoding approach by mitochondrial cytochrome oxidase I subunit (COI) were used to confirm the identification of all species. Present study recorded the first national occurrence of two reef associated Lutjanus species (L. xanthopinnis and L. indicus). In addition six species (L. rivulatus, L. lutjanus, L. vitta, L. lemniscatus, L. fulviflamma and L. johnii) were re-described. Distinct morphological differentiation were found between more closely related species L. xanthopinnis, L. vitta and L. lutjanus. Three different appearances of L. johnii, two different appearances of L. rivulatus and L. lemniscatus were confirmed as same species. Phylogenetic analysis showed monophylotic clade for conspecific individuals and paraphyletic clade for congeneric individuals. Average genetic divergence for intraspecific level was 1.5% and 12.5% for interspecific level. Current study increased the number of Lutjanus species in Bangladesh from 15 to 17 and extended the distribution range of those new recorded species in northeastern part of the Bay of Bengal. Bangladesh J. Zool. 46(2): 239-253, 2018

Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the “Crangonyx floridanus complex”

Zootaxa ◽  
2019 ◽  
Vol 4691 (4) ◽  
pp. 301-332 ◽  
Author(s):  
ANDREW G. CANNIZZARO ◽  
THOMAS R. SAWICKI
Keyword(s):  
New Species ◽  
Related Species ◽  
Species Delimitation ◽  
Species Complex ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Closely Related Species ◽  
Leon County ◽  
Two New Species ◽  
Morphological And Molecular Data

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses. 

Inosperma subsphaerosporum (Inocybaceae), a new species from Hainan, tropical China

Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 169-178
Author(s):  
LUN-SHA DENG ◽  
WEN-JIE YU ◽  
NIAN-KAI ZENG ◽  
LI-JIE LIU ◽  
LI-YUE LIU ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
South Asia ◽  
Related Species ◽  
Hainan Province ◽  
Closely Related Species ◽  
Old World ◽  
Thin Walled ◽  
Tropical China ◽  
A New Species

Inosperma subsphaerosporum sp. nov. is described from Hainan Province of China, based on morphological features and molecular phylogeny. The new species is characterized by a medium-sized basidioma, a scaly-fibrillose to rimulose pileus, subglobose to globose basidiospores, and thin-walled cheilocystidia. Moreover, I. subsphaerosporum is phylogenetically placed in an Old World tropical clade, in which all members described are from South Asia. Detailed descriptions, color photographs as well as comparisons between the new species and its closely related species are provided.

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