scholarly journals Detection of Streptococcus pneumoniae from culture-negative dried blood spots by real-time PCR in Nigerian children with acute febrile illness

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Pui-Ying Iroh Tam ◽  
Nelmary Hernandez-Alvarado ◽  
Mark R. Schleiss ◽  
Amy J. Yi ◽  
Fatimah Hassan-Hanga ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Febrile Illness ◽  
Dried Blood Spots ◽  
Acute Febrile Illness ◽  
Blood Spots ◽  
Nigerian Children ◽  
Culture Negative

scholarly journals Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus

2015 ◽  
Vol 54 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Jie Liu ◽  
Caroline Ochieng ◽  
Steve Wiersma ◽  
Ute Ströher ◽  
Jonathan S. Towner ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Ebola Virus ◽  
Febrile Illness ◽  
Fever Virus ◽  
Outbreak Investigation ◽  
Acute Febrile Illness ◽  
Content Type ◽  
Taqman Array

Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonellaspp.,Brucellaspp.,Coxiella burnetii,Leptospiraspp.,Rickettsiaspp.,Salmonella entericaandSalmonella entericaserovar Typhi, andYersinia pestis), and 3 protozoa (Leishmaniaspp.,Plasmodiumspp., andTrypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.

The use of real-time PCR to detect hepatitis C virus RNA in dried blood spots from Brazilian patients infected chronically

2012 ◽  
Vol 179 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Carlos Santos ◽  
Alexanda Reis ◽  
Cintia Vilhena dos Santos ◽  
Cristine Damas ◽  
Mariliza Henrique Silva ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Real Time ◽  
Real Time Pcr ◽  
Dried Blood Spots ◽  
Hepatitis C Virus Rna ◽  
Blood Spots ◽  
Virus Rna

Dried blood spots versus plasma for the quantitation of HIV-1 RNA using a real-Time PCR, m2000rt assay

2012 ◽  
Vol 181 (2) ◽  
pp. 177-181 ◽  
Author(s):  
Madhavan Vidya ◽  
Shanmugam Saravanan ◽  
Samara Rifkin ◽  
Sunil S. Solomon ◽  
Greer Waldrop ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Hiv 1

scholarly journals Challenges in laboratory diagnosis of Malaria in a low resource country among cases of acute febrile illness at tertiary care hospital in eastern Nepal: Comparative study on Conventional Vs Molecular approach

2020 ◽  
Author(s):  
Pragyan Dahal ◽  
Basudha Khanal ◽  
Keshav Rai ◽  
Vivek Kattel ◽  
Satish Yadav ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Malaria Elimination ◽  
Real Time Pcr ◽  
Gold Standard ◽  
Febrile Illness ◽  
Acute Febrile Illness ◽  
Low Resource ◽  
Elimination Programme ◽  
Low Resource Country

Abstract Background For ongoing malaria elimination programme, available methods like microscopy and rapid diagnostic test (RDT) are not able to detect all the cases of malaria in acute febrile illness. These methods are entirely dependent on the course of infection, parasite load and skilled technical resources thus the study was carried out to compare performance of microscopy and RDT which are commonly used in a low resource country along with reference to real-time PCR. Methods Altogether 52 blood samples collected from patients with acute febrile illness were tested by microscopy, RDT and real-time PCR. The results were compared in terms of sensitivity and specificity. Results The test results were as follows: 5.8% positivity by Microscopy, 13.5% positivity by RDT and 27% by real time PCR. Taking into consideration of PCR as a gold standard method microscopy showed 21.4% sensitivity and 100% specificity. Likewise, RDT results revealed 28.6% sensitivity and 92.1% specificity. Conclusion Despite of various diagnostic tools available, microscopy stills remains the gold standard for the diagnosis and RDT is the user friendly to execute the test under the tree, but our preliminary results emphasized the need to implement the test with the higher sensitivity and specificity in context of malaria elimination programme which could be another important opportunity to understand the parasite circulation in case of low endemic region. However, these results should be further verified with the large study cohort in order to document the submicroscopic infection.

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