scholarly journals Comparative evaluation of BMI-1 proto-oncogene expression in normal tissue, adenoma and papillary carcinoma of human thyroid in pathology samples

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Mohadeseh Hajian ◽  
Abolghasem Esmaeili ◽  
Ardeshir Talebi
Keyword(s):  
Gene Expression ◽  
Papillary Carcinoma ◽  
Integration Site ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
Human Thyroid ◽  
Mrna Levels ◽  
Correct Treatment ◽  
Relative Expression Level ◽  
Thyroid Malignancies

Abstract Objective Papillary Thyroid carcinoma accounts for more than 60% of adult thyroid carcinomas. Finding a helpful marker is vital to determine the correct treatment approach. The present study was aimed to evaluate the expression of the B cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) gene in papillary carcinoma, adenoma, and adjacent healthy thyroid tissues. Pathology blocks of thyroid tissues at the pathology department of patients who have undergone thyroid surgery between 2015 and 2019 were examined; papillary carcinoma, adenoma, and healthy tissues were selected and sectioned. Total RNA was extracted, and the relative expression level of the BMI-1 gene was examined using the Real-Time qPCR method. Results In the papillary and adenoma tissues, BMI-1 was overexpressed (1.047-fold and 1.042-fold) in comparison to healthy tissues (p < 0.05 for both comparisons). However, no statistically significant differences were observed between adenoma and papillary carcinoma tissues regarding BMI-1 gene expression. This study demonstrated a new biomarker for thyroid malignancies and found that the mRNA levels of the BMI-1 gene were higher in tumor tissues compared with healthy tissues. Further studies are needed to evaluate the BMI1 gene expression in other thyroid cancers.

scholarly journals Integration Site Choice of a Feline Immunodeficiency Virus Vector

2006 ◽  
Vol 80 (17) ◽  
pp. 8820-8823 ◽  
Author(s):  
Yubin Kang ◽  
Christopher J. Moressi ◽  
Todd E. Scheetz ◽  
Litao Xie ◽  
Diane Thi Tran ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Integration Site ◽  
Leukemia Virus ◽  
Foamy Virus ◽  
Moloney Murine Leukemia Virus ◽  
Human Hepatoma Cells ◽  
Immunodeficiency Virus ◽  
Integration Sites ◽  
Primate Lentiviruses ◽  
Transcriptional Units

ABSTRACT We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a “bendable” structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

scholarly journals Proviral Integration Site for Moloney Murine Leukemia Virus (PIM) Kinases Promote Human T Helper 1 Cell Differentiation

2012 ◽  
Vol 288 (5) ◽  
pp. 3048-3058 ◽  
Author(s):  
Johanna Tahvanainen ◽  
Minna K. Kyläniemi ◽  
Kartiek Kanduri ◽  
Bhawna Gupta ◽  
Hanna Lähteenmäki ◽  
...  
Keyword(s):  
Murine Leukemia Virus ◽  
Integration Site ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
T Helper ◽  
T Helper 1 ◽  
Proviral Integration ◽  
Proviral Integration Site ◽  
Pim Kinases ◽  
Murine Leukemia

Semi-quantitative analysis of interleukin-1α, interleukin-6 and interleukin-8 mRNA expression by human thyrocytes

1995 ◽  
Vol 15 (1) ◽  
pp. 11-21 ◽  
Author(s):  
P F Watson ◽  
A P Pickerill ◽  
R Davies ◽  
A P Weetman
Keyword(s):  
Gene Expression ◽  
Interleukin 6 ◽  
Interleukin 8 ◽  
Cytokine Gene Expression ◽  
Human Thyroid ◽  
Thyroid Cell ◽  
Mrna Levels ◽  
Cytokine Gene ◽  
Interleukin 1Α ◽  
Ifn Γ

ABSTRACT It has been suggested that the thyroid itself may contribute to the inflammatory process observed in autoimmune thyroiditis by releasing the cytokines interleukin-1α (IL-1α), interleukin-6 (IL-6) and interleukin-8 (IL-8), but studies of cytokine gene expression in thyrocytes have been limited and conflicting. A semi-quantitative reverse transcription-PCR technique has been used to investigate the expression of IL-1α, IL-6 and IL-8 mRNA in the human thyroid cell line HTori3 and in cultures of primary human thyroid follicular cells (TFCs). Cytokine mRNA levels were examined over a 24-h period, and the modulatory effects of exogenous IL-1α, interferon-γ (IFN-γ) and TSH investigated. Basal expression of IL-1α, IL-6 and IL-8 mRNA was detected in HTori3 and primary TFC cultures. Stimulation with IL-1 (10 U/ml) for 12 h produced an increase in the level of IL-1α mRNA in both primary TFC and HTori3 cultures. IL-6 and IL-8 mRNA levels were increased by the addition of IL-1 in both cell types, and this effect was detected throughout the 24-h time-course. IFN-γ (100 U/ml) had no significant effect on cytokine gene expression. A higher concentration of IFN-γ (500 U/ml) had no significant effect on the expression of IL-1α or IL-8 but produced an increase in the level of IL-6 mRNA in primary cultures and in HTori3 cells. Addition of TSH (1 mU/ml) produced an increase in the level of IL-1α mRNA in primary TFC and HTori3 cells, at 12 and 24 h. TSH had no significant effect on the expression of IL-6 or IL-8 mRNA. These results demonstrate that human TFCs constitutively express IL-1α, IL-6 and IL-8 mRNA and that this expression can be modulated by IL-1, IFN-γ and TSH.

scholarly journals Dibenzofuran Derivatives Inspired from Cercosporamide as Dual Inhibitors of Pim and CLK1 Kinases

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6572
Author(s):  
Viet Hung Dao ◽  
Isabelle Ourliac-Garnier ◽  
Cédric Logé ◽  
Florence O. McCarthy ◽  
Stéphane Bach ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Integration Site ◽  
Leukemia Virus ◽  
Docking Studies ◽  
Moloney Murine Leukemia Virus ◽  
Design Synthesis ◽  
Promote Cell Proliferation ◽  
Cellular Assays ◽  
Proviral Integration Site ◽  
Pim Kinases

Pim kinases (proviral integration site for Moloney murine leukemia virus kinases) are overexpressed in various types of hematological malignancies and solid carcinomas, and promote cell proliferation and survival. Thus, Pim kinases are validated as targets for antitumor therapy. In this context, our combined efforts in natural product-inspired library generation and screening furnished very promising dibenzo[b,d]furan derivatives derived from cercosporamide. Among them, lead compound 44 was highlighted as a potent Pim-1/2 kinases inhibitor with an additional nanomolar IC50 value against CLK1 (cdc2-like kinases 1) and displayed a low micromolar anticancer potency towards the MV4-11 (AML) cell line, expressing high endogenous levels of Pim-1/2 kinases. The design, synthesis, structure–activity relationship, and docking studies are reported herein and supported by enzyme, cellular assays, and Galleria mellonella larvae testing for acute toxicity.

scholarly journals A Chimeric Ty3/Moloney Murine Leukemia Virus Integrase Protein Is Active In Vivo

1998 ◽  
Vol 72 (5) ◽  
pp. 4297-4307 ◽  
Author(s):  
Sandra L. Dildine ◽  
James Respess ◽  
Doug Jolly ◽  
Suzanne B. Sandmeyer
Keyword(s):  
Cell Line ◽  
Murine Leukemia Virus ◽  
Genomic Dna ◽  
Integration Site ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
Packaging Cell Line ◽  
Terminal Domain ◽  
Murine Leukemia

ABSTRACT This report describes the results of experiments to determine whether chimeras between a retrovirus and portions of Ty3 are active in vivo. A chimera between Ty3 and a Neor-marked Moloney murine leukemia virus (M-MuLV) was constructed. The C-terminal domain of M-MuLV integrase (IN) was replaced with the C-terminal domain of Ty3 IN. The chimeric retroviruses were expressed from an amphotrophic envelope packaging cell line. The virus generated was used to infect the human fibrosarcoma cell line HT1080, and cells in which integration had occurred were selected by G418 resistance. Three independently integrated viruses were rescued. In each case, the C-terminal Ty3 IN sequences were maintained and short direct repeats of the genomic DNA flanked the integration site. Sequence analysis of the genomic DNA flanking the insertion did not identify a tRNA gene; therefore, these integration events did not have Ty3 position specificity. This study showed that IN sequences from the yeast retrovirus-like element Ty3 can substitute for M-MuLV IN sequences in the C-terminal domain and contribute to IN function in vivo. It is also one of the first in vivo demonstrations of activity of a retrovirus encoding an integrase chimera. Studies of chimeras between IN species with distinctive integration patterns should complement previous work by expanding our understanding of the roles of nonconserved domains.

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