Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering

Abstract Objective We aimed to detect the effect of a couple of parameters including Alg, H2O2, and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure. Results NMR, UV–Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone. Data showed gelation time was delayed with the increase and reduction of H2O2 and HRP, respectively. We noted that hydrogel consisted of 1.2% (w/v) Alg, 5 U HRP, and 100 mM H2O2 yielded an appropriate gelation time with appropriate mechanical properties. The addition of 0.5% (v/v) Col developed hydrogel increased the gelation time. The data showed that Alg, HRP, and H2O2 with the ratio of 1:0.54:0.54 had proper physicochemical features for cartilage engineering.

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Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

Biomolecules ◽

10.3390/biom10101456 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1456

Author(s):

Isabel Matos Oliveira ◽

Cristiana Gonçalves ◽

Myeong Eun Shin ◽

Sumi Lee ◽

Rui Luis Reis ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Mechanical Properties ◽

Silk Fibroin ◽

Therapeutic Efficacy ◽

Composite Structures ◽

Gelation Time ◽

Polymeric Materials ◽

Gellan Gum ◽

Traditional Use

Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty–GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Ty–GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2–5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Ty–GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Ty–GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.

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Generation of Photopolymerized Microparticles Based on PEGDA Using Microfluidic Devices. Part 1. Initial Gelation Time and Mechanical Properties of the Material

Micromachines ◽

10.3390/mi12030293 ◽

2021 ◽

Vol 12 (3) ◽

pp. 293

Author(s):

José M. Acosta-Cuevas ◽

José González-García ◽

Mario García-Ramírez ◽

Víctor H. Pérez-Luna ◽

Erick Omar Cisneros-López ◽

...

Keyword(s):

Mechanical Properties ◽

Gelation Time ◽

Mesh Size ◽

Microfluidic Devices ◽

Future Generation ◽

Mechanical Tests ◽

Continuous Systems ◽

Polyethylene Glycol Diacrylate ◽

Glycol Diacrylate ◽

Close Relationship

Photopolymerized microparticles are made of biocompatible hydrogels like Polyethylene Glycol Diacrylate (PEGDA) by using microfluidic devices are a good option for encapsulation, transport and retention of biological or toxic agents. Due to the different applications of these microparticles, it is important to investigate the formulation and the mechanical properties of the material of which they are made of. Therefore, in the present study, mechanical tests were carried out to determine the swelling, drying, soluble fraction, compression, cross-linking density (Mc) and mesh size (ξ) properties of different hydrogel formulations. Tests provided sufficient data to select the best formulation for the future generation of microparticles using microfluidic devices. The initial gelation times of the hydrogels formulations were estimated for their use in the photopolymerization process inside a microfluidic device. Obtained results showed a close relationship between the amount of PEGDA used in the hydrogel and its mechanical properties as well as its initial gelation time. Consequently, it is of considerable importance to know the mechanical properties of the hydrogels made in this research for their proper manipulation and application. On the other hand, the initial gelation time is crucial in photopolymerizable hydrogels and their use in continuous systems such as microfluidic devices.

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The influence of mechanical properties of subchondral plate, femoral head and neck on dynamic stress distribution of the articular cartilage

Medical Engineering & Physics ◽

10.1016/j.medengphy.2004.12.008 ◽

2005 ◽

Vol 27 (4) ◽

pp. 295-304 ◽

Cited By ~ 41

Author(s):

Hung-Wen Wei ◽

Shih-Sheng Sun ◽

Shyh-Hua Eric Jao ◽

Chiuan-Ren Yeh ◽

Cheng-Kung Cheng

Keyword(s):

Mechanical Properties ◽

Articular Cartilage ◽

Femoral Head ◽

Stress Distribution ◽

Head And Neck ◽

Dynamic Stress ◽

Subchondral Plate ◽

Dynamic Stress Distribution

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The generalized triphasic correspondence principle for simultaneous determination of the mechanical properties and proteoglycan content of articular cartilage by indentation

Journal of Biomechanics ◽

10.1016/j.jbiomech.2006.11.015 ◽

2007 ◽

Vol 40 (11) ◽

pp. 2434-2441 ◽

Cited By ~ 23

Author(s):

X. Lux Lu ◽

Chester Miller ◽

Faye H. Chen ◽

X. Edward Guo ◽

Van C. Mow

Keyword(s):

Mechanical Properties ◽

Articular Cartilage ◽

Simultaneous Determination ◽

Correspondence Principle ◽

Proteoglycan Content

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Sequential changes in the mechanical properties of viable articular cartilage storedin vitro

Journal of Orthopaedic Research® ◽

10.1002/jor.1100020109 ◽

1984 ◽

Vol 2 (1) ◽

pp. 55-60 ◽

Cited By ~ 13

Author(s):

Victor J. Thomas ◽

Sergio A. Jimenez ◽

Carl T. Brighton ◽

Norman Brown

Keyword(s):

Mechanical Properties ◽

Articular Cartilage

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Transversely isotropic micromechanics model to determine effect of collagen fibre angle in mechanical properties of articular cartilage

Materials Technology ◽

10.1179/1753555714y.0000000178 ◽

2014 ◽

Vol 29 (6) ◽

pp. 377-383 ◽

Cited By ~ 1

Author(s):

S. Mohammad Mehdi Elhamian ◽

M. Alizadeh ◽

M. Mehrdad Shokrieh ◽

A. Karimi ◽

S. Pejman Madani

Keyword(s):

Mechanical Properties ◽

Articular Cartilage ◽

Collagen Fibre ◽

Transversely Isotropic ◽

Micromechanics Model ◽

Fibre Angle ◽

Determine Effect

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Permeability of Human Glenohumeral Joint Cartilage

10.1115/imece1999-0462 ◽

1999 ◽

Author(s):

Anna Stankiewicz ◽

Gerard A. Ateshian ◽

Louis U. Bigliani ◽

Van C. Mow

Keyword(s):

Mechanical Properties ◽

Articular Cartilage ◽

Interstitial Fluid ◽

Glenohumeral Joint ◽

Solid Matrix ◽

Joint Cartilage ◽

Frictional Drag ◽

Diarthrodial Joints ◽

Flow Through ◽

Fluid Pressurization

Abstract The nearly frictionless lubrication in diarthrodial joints and load support within articular cartilage depends on its mechanical properties. It has been shown that the majority of applied loads on cartilage are supported by interstitial fluid pressurization (Ateshian et al., 1994) which results from the frictional drag of flow through the porous permeable solid matrix. The duration and magnitude of this pressurization are a function of the permeability of cartilage (Lai et al., 1981).

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Redox-Sensitive Ultrashort Peptide Hydrogel with Tunable Mechanical Properties for Anti-Tumor Drug Delivery

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2020.2974 ◽

2020 ◽

Vol 16 (11) ◽

pp. 1588-1599

Author(s):

Yiping Li ◽

Ying Zhu ◽

Shiyao Luo ◽

Yue He ◽

Zhewei Huang ◽

...

Keyword(s):

Mechanical Properties ◽

Targeted Delivery ◽

Subcutaneous Tissue ◽

Drug Loading ◽

Gelation Time ◽

In Vivo Studies ◽

Dependent Manner ◽

The Cross ◽

Hydrogel System

In this study, we report a new ultrashort peptide (LOC), which forms a redox-sensitive hydrogel after cross-linking with the mild oxidant H2 O2 and used it for tumor-targeted delivery of doxorubicin hydrochloride (DOX). LOC gelled within a few minutes in low-concentration H2 O2 solution. The concentration of H2 O2 significantly altered the gelation time and mechanical properties of the hydrogel. The in vitro micromorphology, secondary structure and rheology characterization of cross-linked hydrogels confirmed the sensitivity and injectability to reducing agent. The cross-linked hydrogel had a strong drug loading capacity, and the drug was released in a GSH concentration-dependent manner, following the Fick diffusion model. In addition, the cross-linked hydrogel showed no cytotoxicity to normal fibroblasts, and no damage to the subcutaneous tissue of mice was observed. In vitro cytotoxicity experiments showed that the DOX-hydrogel system exhibited good anti-cancer efficacy. In vivo studies using 4T1 tumor-bearing mice showed that the DOX-hydrogel system had a significant inhibitory effect on tumors. Therefore, the newly designed redox-sensitive hydrogel can effectively enhance the therapeutic efficacy of DOX and reduce toxicity, making it an attractive biological material.

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