scholarly journals Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiaomeng Pei ◽  
Junchi Liu ◽  
Mingxing Liu ◽  
Hong Zhou ◽  
Xiaomin Wang ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Bioinformatics Analysis ◽  
Isotope Labeling ◽  
Streptococcus Suis ◽  
Streptococcal Toxic Shock Syndrome ◽  
Liquid Chromatography Mass Spectrometry ◽  
Toxic Shock ◽  
Polymerase Chain ◽  
Suis Serotype ◽  
Shed Light

Abstract Streptococcus suis serotype 2 (SS2) is a serious zoonotic pathogen; it can lead to symptoms of streptococcal toxic shock syndrome (STSS) in humans and sepsis in pigs, and poses a great threat to public health. The SS2 MetQ gene deletion strain has attenuated antiphagocytosis, although the mechanism of antiphagocytosis and pathogenesis of MetQ in SS2 has remained unclear. In this study, stable isotope labeling by amino acids in cell culture (SILAC) based liquid chromatography–mass spectrometry (LC–MS) and subsequent bioinformatics analysis was used to determine differentially expressed proteins of RAW264.7 cells infected with △MetQ and ZY05719. Proteomic results were verified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting for selected proteins. Further research was focused mainly on immune system processes related to downregulated proteins, such as Src and Ccl9, and actin cytoskeleton and endocytosis related upregulated proteins, like Pstpip1 and Ppp1r9b. The proteomic results in this study shed light on the mechanism of antiphagocytosis and innate immunity of macrophages infected with △MetQ and ZY05719, which might provide novel targets to prevent or control the infection of SS2.

scholarly journals Streptococcal Toxic Shock Syndrome Caused by Streptococcus suis Serotype 2

PLoS Medicine ◽  
2006 ◽  
Vol 3 (5) ◽  
pp. e151 ◽  
Author(s):  
Jiaqi Tang ◽  
Changjun Wang ◽  
Youjun Feng ◽  
Weizhong Yang ◽  
Huaidong Song ◽  
...  
Keyword(s):  
Toxic Shock Syndrome ◽  
Streptococcus Suis ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

scholarly journals Correction: Streptococcal Toxic Shock Syndrome Caused by Streptococcus suis Serotype 2

PLoS Medicine ◽  
2006 ◽  
Vol 3 (8) ◽  
pp. e377 ◽  
Author(s):  
Jiaqi Tang ◽  
Changjun Wang ◽  
Youjun Feng ◽  
Weizhong Yang ◽  
Huaidong Song ◽  
...  
Keyword(s):  
Toxic Shock Syndrome ◽  
Streptococcus Suis ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

scholarly journals The Orphan Response Regulator CovR: a Globally Negative Modulator of Virulence in Streptococcus suis Serotype 2

2009 ◽  
Vol 191 (8) ◽  
pp. 2601-2612 ◽  
Author(s):  
Xiuzhen Pan ◽  
Junchao Ge ◽  
Ming Li ◽  
Bo Wu ◽  
Changjun Wang ◽  
...  
Keyword(s):  
Profile Analysis ◽  
Response Regulator ◽  
Streptococcus Suis ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Full Agreement ◽  
Virulence Regulation ◽  
Wide Range ◽  
Life Threatening ◽  
Suis Serotype

ABSTRACT Streptococcus suis serotype 2 is an emerging zoonotic pathogen responsible for a wide range of life-threatening diseases in pigs and humans. However, the pathogenesis of S. suis serotype 2 infection is not well understood. In this study, we report that an orphan response regulator, CovR, globally regulates gene expression and negatively controls the virulence of S. suis 05ZYH33, a streptococcal toxic shock syndrome (STSS)-causing strain. A covR-defective (ΔcovR) mutant of 05ZYH33 displayed dramatic phenotypic changes, such as formation of longer chains, production of thicker capsules, and increased hemolytic activity. Adherence of the ΔcovR mutant to epithelial cells was greatly increased, and its resistance to phagocytosis and killing by neutrophils and monocytes was also significantly enhanced. More importantly, inactivation of covR increased the lethality of S. suis serotype 2 in experimental infection of piglets, and this phenotype was restored by covR complementation. Colonization experiments also showed that the ΔcovR mutant exhibited an increased ability to colonize susceptible tissues of piglets. The pleiotropic phenotype of the ΔcovR mutant is in full agreement with the large number of genes controlled by CovR as revealed by transcription profile analysis: 2 genes are positively regulated, and 193 are repressed, including many that encode known or putative virulence factors. These findings suggested that CovR is a global repressor in virulence regulation of STSS-causing S. suis serotype 2.

Streptococcus suis serotype 2 caused streptococcal toxic shock syndrome(STSS) in a patient

2008 ◽  
Vol 22 (5) ◽  
pp. 313-316 ◽  
Author(s):  
Yefei Zhu ◽  
Zhongmin Tan ◽  
Lingyang Zhu ◽  
Hongxing Pan ◽  
Xuejun Zhang ◽  
...  
Keyword(s):  
Toxic Shock Syndrome ◽  
Streptococcus Suis ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

scholarly journals Functional Definition and Global Regulation of Zur, a Zinc Uptake Regulator in a Streptococcus suis Serotype 2 Strain Causing Streptococcal Toxic Shock Syndrome

2008 ◽  
Vol 190 (22) ◽  
pp. 7567-7578 ◽  
Author(s):  
Youjun Feng ◽  
Ming Li ◽  
Huimin Zhang ◽  
Beiwen Zheng ◽  
Huiming Han ◽  
...  
Keyword(s):  
Toxic Shock Syndrome ◽  
Streptococcus Suis ◽  
Gene Replacement ◽  
Zinc Uptake ◽  
Zinc Homeostasis ◽  
Differentially Expressed ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock ◽  
Physiological Tests ◽  
Zinc Uptake Regulator

ABSTRACT Zinc is an essential trace element for all living organisms and plays pivotal roles in various cellular processes. However, an excess of zinc is extremely deleterious to cells. Bacteria have evolved complex machineries (such as efflux/influx systems) to control the concentration at levels appropriate for the maintenance of zinc homeostasis in cells and adaptation to the environment. The Zur (zinc uptake regulator) protein is one of these functional members involved in the precise control of zinc homeostasis. Here we identified a zur homologue designated 310 from Streptococcus suis serotype 2, strain 05ZYH33, a highly invasive isolate causing streptococcal toxic shock syndrome. Biochemical analysis revealed that the protein product of gene 310 exists as a dimer form and carries zinc ions. An isogenic gene replacement mutant of gene 310, the Δ310 mutant, was obtained by homologous recombination. Physiological tests demonstrated that the Δ310 mutant is specifically sensitive to Zn2+, while functional complementation of the Δ310 mutant can restore its duration capability, suggesting that 310 is a functional member of the Zur family. Two-dimensional electrophoresis indicated that nine proteins in the Δ310 mutant are overexpressed in comparison with those in the wild type. DNA microarray analyses suggested that 121 genes in the Δ310 mutant are affected, of which 72 genes are upregulated and 49 are downregulated. The transcriptome of S. suis serotype 2 with high Zn2+ concentrations also showed 117 differentially expressed genes, with 71 upregulated and 46 downregulated. Surprisingly, more than 70% of the genes differentially expressed in the Δ310 mutant were the same as those in S. suis serotype 2 that were differentially expressed in response to high Zn2+ concentration, consistent with the notion that 310 is involved in zinc homeostasis. We thus report for the first time a novel zinc-responsive regulator, Zur, from Streptococcus suis serotype 2.

DETECTION OF STREPTOCOCCUS SUIS SEROTYPE 2 IN CEREBROSPINAL FLUID (CFS) SAMPLES BY A REALTIME POLYMERASE CHAIN REACTION

2016 ◽  
pp. 94-98
Author(s):  
Van An Le ◽  
Hoang Bach Nguyen ◽  
Nu Dieu Hong Phan
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Culture ◽  
Streptococcus Suis ◽  
Bacterial Isolation ◽  
Chain Reaction ◽  
Realtime Pcr ◽  
Purulent Meningitis ◽  
Polymerase Chain ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

Objective: To apply a realtime PCR for detection of Streptococcus suis serotype 2 in CSF samples from patients with purulent meningitis. Methodology: Fifty five CSF samples from patients with suspected meningitis were collected for testing by the realtime PCR amplified for cps2J gene and bacterial isolation. Results: The results showed that S.suis was positve in 21 samples (38.2%) by realtime PCR, in which 18 samples (32.7%) were positive by both realtime PCR and bacterial culture, 3 samples (5.5%) were positive by only realtime PCR. Conclusion: The realtime PCR detected S.suis with higher rate than bacterial isolation, it should be used routinely for detection of meningitis caused by S.suis in laboratories equipped with PCR system. Key words: Liên cầu lợn (Streptococcus suis), meningitis, CSF, realtime PCR

scholarly journals Identification of Three Type II Toxin-Antitoxin Systems in Streptococcus suis Serotype 2

Toxins ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 467 ◽  
Author(s):  
Jiali Xu ◽  
Nian Zhang ◽  
Manman Cao ◽  
Sujing Ren ◽  
Ting Zeng ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bioinformatics Analysis ◽  
Streptococcus Suis ◽  
Type Ii ◽  
Bacterial Genomes ◽  
Functional Studies ◽  
Pathogen Virulence ◽  
Typical Type ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

Type II toxin-antitoxin (TA) systems are highly prevalent in bacterial genomes and have been extensively studied. These modules involve in the formation of persistence cells, the biofilm formation, and stress resistance, which might play key roles in pathogen virulence. SezAT and yefM-yoeB TA modules in Streptococcus suis serotype 2 (S. suis 2) have been studied, although the other TA systems have not been identified. In this study, we investigated nine putative type II TA systems in the genome of S. suis 2 strain SC84 by bioinformatics analysis and identified three of them (two relBE loci and one parDE locus) that function as typical type II TA systems. Interestingly, we found that the introduction of the two RelBE TA systems into Escherichia coli or the induction of the ParE toxin led to cell filamentation. Promoter activity assays indicated that RelB1, RelB2, ParD, and ParDE negatively autoregulated the transcriptions of their respective TA operons, while RelBE2 positively autoregulated its TA operon transcription. Collectively, we identified three TA systems in S. suis 2, and our findings have laid an important foundation for further functional studies on these TA systems.

A fatal case of streptococcal toxic shock syndrome caused by Streptococcus suis carrying tet (40) and tet (O/W/32/O), Italy

2016 ◽  
Vol 22 (11) ◽  
pp. 774-776 ◽  
Author(s):  
Fabiola Mancini ◽  
Francesco Adamo ◽  
Roberta Creti ◽  
Monica Monaco ◽  
Giovanna Alfarone ◽  
...  
Keyword(s):  
Toxic Shock Syndrome ◽  
Fatal Case ◽  
Streptococcus Suis ◽  
Streptococcal Toxic Shock Syndrome ◽  
Toxic Shock
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