Upregulation of CKIP-1 inhibits high-glucose induced inflammation and oxidative stress in HRECs and attenuates diabetic retinopathy by modulating Nrf2/ARE signaling pathway: an in vitro study

Protein glycation and oxidative stress lead to severe health complications in various diseases including diabetes mellitus. The intake of flavonoid-rich foods has been confirmed previously to have a positive effect on human health. Ginger is an important source of flavonoids and is one of the most widely used traditional medicines in many Asian countries. The aim of this study was to verify the therapeutic potential of methanolic extract from ginger against glycation and other oxidative stress-induced complications using in vitro study. In this study, quantitative estimations of antioxidant components such as total phenolic and flavonoids were determined by UV–visible spectrophotometry. The anti-inflammatory action of the ginger extract was checked by determining its protective action against the denaturation of proteins, anti-proteinase activity and its membrane stabilization effect. The anti-inflammatory action of ginger extract was found to be comparable with reference standard drugs. The antiglycating effect of ginger extract was investigated by placing bovine serum albumin (BSA) with glucose in the presence and absence of ginger extract for two weeks at 37 °C. The incubated samples were analyzed for the number of glycation products, secondary structural changes, aggregation and advanced glycation end products (AGEs) formation by checking browning intensity, determination of aggregation index and Congo red assays. Our findings demonstrated that ginger extract (600 µg/mL) significantly reduced the browning, secondary structural changes, aggregation and AGEs formation. Thus, it can be concluded from these results that ginger extract is a wealthy source of antioxidants and can be used to prevent the glycation and oxidative stress-induced damage of biomolecules in various health complications including inflammation.

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Thiram-induced cytotoxicity and oxidative stress in human erythrocytes: an in vitro study

Pesticide Biochemistry and Physiology ◽

10.1016/j.pestbp.2019.12.003 ◽

2020 ◽

Vol 164 ◽

pp. 14-25

Author(s):

Samreen Salam ◽

Amin Arif ◽

Riaz Mahmood

Keyword(s):

Oxidative Stress ◽

In Vitro Study ◽

Human Erythrocytes ◽

Vitro Study ◽

And Oxidative Stress

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Cardio-toxicity and oxidative stress induced by Egyptian Cyanobacteria Oscillatoria brevis, toxicity and mechanism of action, an in vitro study

IOSR Journal of Pharmacy and Biological Sciences ◽

10.9790/3008-09429099 ◽

2014 ◽

Vol 9 (4) ◽

pp. 90-99

Author(s):

Mentalla M. Mohammed ◽

◽

Sherifa H. Ahmed ◽

Abeer S. Amin ◽

Zohour N. Ibrahim ◽

...

Keyword(s):

Oxidative Stress ◽

Mechanism Of Action ◽

In Vitro Study ◽

Vitro Study ◽

And Oxidative Stress

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Interactions Between Dental Composite Resins and Saliva A comparative biochemical in vitro study

Materiale Plastice ◽

10.37358/mp.19.3.5223 ◽

2019 ◽

Vol 56 (3) ◽

pp. 529-533

Author(s):

Mihaela Pantea ◽

Diana Andreea Ighigeanu ◽

Alexandra Totan ◽

Maria Greabu ◽

Daniela Miricescu ◽

...

Keyword(s):

Oxidative Stress ◽

In Vitro Study ◽

Composite Resins ◽

Vitro Study ◽

Dental Composite ◽

Gamma Glutamyl Transferase ◽

Specific Protocol ◽

Dental Composite Resins ◽

Glutamyl Transferase

This in vitro study analyses the biochemical interaction between saliva and three types of dental composite resins (a direct resin, an indirect resin and a dual-cure resin used for cementation of indirect dental restorations). The resin samples were obtained following a specific protocol and in line with the producers� recommendations; the resin samples were incubated with saliva samples collected from 19 healthy volunteers. The obtained results showed that the tested composite resins did not produce significant changes in oxidative stress parameters that were analysed (albumin, uric acid, GGT / gamma glutamyl transferase, OXSR-1 / oxidative stress responsive kinase 1) and do not influence the inflammatory salivary status reflected by the levels of IL-6 - an inflammatory marker.

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Oxi-Redox Selective Breast Cancer Treatment: An In Vitro Study of Theranostic In-Based Oxide Nanoparticles for Controlled Generation or Prevention of Oxidative Stress

ACS Applied Materials & Interfaces ◽

10.1021/acsami.0c17326 ◽

2021 ◽

Author(s):

Nai-Sheng Hsu ◽

Moeava Tehei ◽

Md Shahriar Hossain ◽

Anatoly Rosenfeld ◽

Muhammad J. A. Shiddiky ◽

...

Keyword(s):

Breast Cancer ◽

Oxidative Stress ◽

Cancer Treatment ◽

In Vitro Study ◽

Breast Cancer Treatment ◽

Vitro Study ◽

Oxide Nanoparticles

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Tetrahydrocurcumin Ameliorates Diabetic Cardiomyopathy by Attenuating High Glucose-Induced Oxidative Stress and Fibrosis via Activating the SIRT1 Pathway

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/6746907 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15 ◽

Cited By ~ 12

Author(s):

Kaifeng Li ◽

Mengen Zhai ◽

Liqing Jiang ◽

Fan Song ◽

Bin Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Signaling Pathway ◽

Diabetic Cardiomyopathy ◽

High Glucose ◽

Therapeutic Potential ◽

Ros Generation ◽

Protective Effects ◽

Collagen Iii

Hyperglycemia-induced oxidative stress and fibrosis play a crucial role in the development of diabetic cardiomyopathy (DCM). Tetrahydrocurcumin (THC), a major bioactive metabolite of natural antioxidant curcumin, is reported to exert even more effective antioxidative and superior antifibrotic properties as well as anti-inflammatory and antidiabetic abilities. This study was designed to investigate the potential protective effects of THC on experimental DCM and its underlying mechanisms, pointing to the role of high glucose-induced oxidative stress and interrelated fibrosis. In STZ-induced diabetic mice, oral administration of THC (120 mg/kg/d) for 12 weeks significantly improved the cardiac function and ameliorated myocardial fibrosis and cardiac hypertrophy, accompanied by reduced reactive oxygen species (ROS) generation. Mechanically, THC administration remarkably increased the expression of the SIRT1 signaling pathway both in vitro and in vivo, further evidenced by decreased downstream molecule Ac-SOD2 and enhanced deacetylated production SOD2, which finally strengthened antioxidative stress capacity proven by repaired activities of SOD and GSH-Px and reduced MDA production. Additionally, THC treatment accomplished its antifibrotic effect by depressing the ROS-induced TGFβ1/Smad3 signaling pathway followed by reduced expression of cardiac fibrotic markers α-SMA, collagen I, and collagen III. Collectively, these finds demonstrated the therapeutic potential of THC treatment to alleviate DCM mainly by attenuating hyperglycemia-induced oxidative stress and fibrosis via activating the SIRT1 pathway.

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