In vitro antibacterial effect of fifth generation dentin bonding agent incorporated with nisin on Streptococcus mutans

Abstract Introduction Bacterial adherence to restorative materials such as composite resin is one of the aetiology of secondary caries. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with nisin, against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the experimental bonding agents were obtained using 250 μl Brain Heart Infusion (BHI) broth and the groups were control (BA with 0% Nisin), bonding agent with 1 wt% (NBA 1) and 5 wt% nisin (NBA 5). To this, 10 μl S. mutans culture was added and incubated at 37 °C. Bacterial growth was estimated by changes in optical density using spectrophotometer every 20 min for 2 h. The results were statistically analysed using one way ANOVA followed by Tukey Post Hoc test. For adherence and membrane integrity test, 10 μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 h. After rinsing, 1 ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under confocal laser scanning microscope (CLSM) for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals with maximum value at 2 h (0.83 ± 0.008), while there was a concentration dependant reduction in bacterial growth with the NBA groups (0.50 ± 0.007). Correspondingly, the NBA groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent nisin in dentin bonding agent may render the resin dentin interface more resistant to bacterial penetration, and adherence of cariogenic bacteria like S. mutans.

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In Vitro Antibacterial Effect of Fifth Generation Dentin Bonding Agent Incorporated With Nisin on Streptococcus Mutans

10.21203/rs.3.rs-495372/v1 ◽

2021 ◽

Author(s):

Gopal Keerthipriya ◽

Nesamani Ravikumar ◽

Sekar Mahalaxmi

Keyword(s):

Streptococcus Mutans ◽

Optical Density ◽

Bacterial Growth ◽

Membrane Integrity ◽

Bonding Agent ◽

Control Group ◽

Integrity Test ◽

Secondary Caries ◽

Fifth Generation ◽

Live Bacteria

Abstract Introduction Secondary caries is the most common cause of failure of composite restorations. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with Nisin, a polypeptide bacteriocin against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the three experimental bonding agents, namely control BA only, bonding agent with 1 wt% Nisin (NBA 1) and 5 wt% Nisin (NBA 5) were obtained using 250 μl Brain Heart Infusion (BHI) broth. To this, 10 μl S. mutans culture was added and incubated at 37°C. Bacterial growth was estimated by changes in optical density using spectrophotometry every 20 min for 2 hours. The results were statistically analysed using one way ANOVA followed by Post Hoc test. For adherence and membrane integrity test, 10μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 hours. After rinsing, 1ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under CLSM for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals, with experimental groups showing concentration dependant reduction in bacterial growth. Correspondingly, the experimental groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent Nisin in bonding agent may render the resin dentin interface more resistant to bacterial penetration, thus reducing the incidence of secondary caries.

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Pre-application of dentin bonding agent prevents discoloration caused by mineral trioxide aggregate

10.21203/rs.2.20732/v3 ◽

2020 ◽

Author(s):

Yoo-Lim Choi ◽

Young-Eun Jang ◽

Bom Sahn Kim ◽

Jin-Woo Kim ◽

Yemi Kim

Keyword(s):

Statistical Significance ◽

Mineral Trioxide Aggregate ◽

Bonding Agent ◽

Control Group ◽

P Value ◽

Dentin Bonding ◽

Color Changes ◽

Tooth Discoloration ◽

Significant Difference ◽

Dentin Bonding Agent

Abstract Background: To evaluate tooth discoloration by newly developed calcium silicate-based materials, and to examine the pre-application of dentin bonding agent (DBA) for preventing discoloration caused by mineral trioxide aggregate (MTA). Methods: The roots of 50 premolars were randomly divided into five groups (n=10) and cavities were prepared from resected root surfaces. MTA was placed in the cavities of teeth belonging to the ProRoot MTA (MTA) and RetroMTA (RMTA) groups. For teeth belonging to the ProRoot + DBA (MTA-B) and RetroMTA + DBA (RMTA-B) groups, DBA was first applied to the cavities prior to the addition of MTA. Teeth in the control group were restored with composite resin only (i.e., without MTA). After 12 weeks, MTA was removed from the MTA and RMTA groups and bleaching agents were applied for 3 additional weeks. Color assessments were recorded at baseline, and 1, 4, and 12 weeks, as well as after bleaching. A one-way ANOVA was performed to assess the differences between the two types of MTAs and color changes following DBA pre-application in each MTA group. A p-value of < 0.05 was considered indicative of statistical significance. Results: Following 12 weeks of MTA treatment, there was a significant difference between the discoloration in the MTA and RMTA groups ( p < 0.05). However, no significant difference was observed between the RMTA and RMTA-B groups ( p > 0.05). Following bleaching, the color changes (ΔE values) of the MTA group were not significantly different from those of the MTA-B group ( p > 0.05). The difference of ΔE between the RMTA group after internal bleaching and the RMTA-B group was also not significant ( p > 0.05). Conclusions: RetroMTA caused significantly less discoloration than ProRoot MTA. Pre-application of DBA reduced discoloration caused by ProRoot MTA. MTA discoloration was improved equally well between DBA pre-application and post-bleaching. Clinical Relevance: Tooth discoloration by mineral trioxide aggregate (MTA) limits its use in esthetic regions. The pre-application of dentin bonding agent (DBA) and the use of newly developed RetroMTA could help to prevent the discoloration caused by MTA.

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Pre-application of dentin bonding agent prevents discoloration caused by mineral trioxide aggregate

10.21203/rs.2.20732/v4 ◽

2020 ◽

Author(s):

Yoo-Lim Choi ◽

Young-Eun Jang ◽

Bom Sahn Kim ◽

Jin-Woo Kim ◽

Yemi Kim

Keyword(s):

Statistical Significance ◽

Mineral Trioxide Aggregate ◽

Bonding Agent ◽

Control Group ◽

P Value ◽

Dentin Bonding ◽

Color Changes ◽

Tooth Discoloration ◽

Significant Difference ◽

Dentin Bonding Agent

Abstract Background: To evaluate tooth discoloration by newly developed calcium silicate-based materials, and to examine the pre-application of dentin bonding agent (DBA) for preventing discoloration caused by mineral trioxide aggregate (MTA).Methods: The roots of 50 premolars were randomly divided into five groups (n=10) and cavities were prepared from resected root surfaces. MTA was placed in the cavities of teeth belonging to the ProRoot MTA (MTA) and RetroMTA (RMTA) groups. For teeth belonging to the ProRoot + DBA (MTA-B) and RetroMTA + DBA (RMTA-B) groups, DBA was first applied to the cavities prior to the addition of MTA. Teeth in the control group were restored with composite resin only (i.e., without MTA). After 12 weeks, MTA was removed from the MTA and RMTA groups and bleaching agents were applied for 3 additional weeks. Color assessments were recorded at baseline, and 1, 4, and 12 weeks, as well as after bleaching. A one-way ANOVA was performed to assess the differences between the two types of MTAs and color changes following DBA pre-application in each MTA group. A p-value of < 0.05 was considered indicative of statistical significance.Results: Following 12 weeks of MTA treatment, there was a significant difference between the discoloration in the MTA and RMTA groups (p < 0.05). However, no significant difference was observed between the RMTA and RMTA-B groups (p > 0.05). Following bleaching, the color changes (ΔE values) of the MTA group were not significantly different from those of the MTA-B group (p > 0.05). The difference of ΔE between the RMTA group after internal bleaching and the RMTA-B group was also not significant (p > 0.05).Conclusions: RetroMTA caused significantly less discoloration than ProRoot MTA. Pre-application of DBA reduced discoloration caused by ProRoot MTA. MTA discoloration was improved equally well between DBA pre-application and post-bleaching.

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Pre-application of dentin bonding agent prevents discoloration caused by mineral trioxide aggregate

10.21203/rs.2.20732/v2 ◽

2020 ◽

Author(s):

Yoo-Lim Choi ◽

Young-Eun Jang ◽

Bom Sahn Kim ◽

Jin-Woo Kim ◽

Yemi Kim

Keyword(s):

Statistical Significance ◽

Mineral Trioxide Aggregate ◽

Bonding Agent ◽

Control Group ◽

P Value ◽

Dentin Bonding ◽

Color Changes ◽

Tooth Discoloration ◽

Significant Difference ◽

Dentin Bonding Agent

Abstract Background: To evaluate tooth discoloration by newly developed calcium silicate-based materials, and to examine the pre-application of dentin bonding agent (DBA) for preventing discoloration caused by mineral trioxide aggregate (MTA).Methods: The roots of 50 premolars were randomly divided into five groups (n=10) and cavities were prepared from resected root surfaces. MTA was placed in the cavities of teeth belonging to the ProRoot MTA (MTA) and RetroMTA (RMTA) groups. For teeth belonging to the ProRoot + DBA (MTA-B) and RetroMTA + DBA (RMTA-B) groups, DBA was first applied to the cavities prior to the addition of MTA. Teeth in the control group were restored with composite resin only (i.e., without MTA). After 12 weeks, MTA was removed from the MTA and RMTA groups and bleaching agents were applied for 3 additional weeks. Color assessments were recorded at baseline, and 1, 4, and 12 weeks, as well as after bleaching. A one-way ANOVA was performed to assess the differences between the two types of MTAs and color changes following DBA pre-application in each MTA group. A p-value of < 0.05 was considered indicative of statistical significance.Results: Following 12 weeks of MTA treatment, there was a significant difference between the discoloration in the MTA and RMTA groups (p < 0.05). However, no significant difference was observed between the RMTA and RMTA-B groups (p > 0.05). Following bleaching, the color changes (ΔE values) of the MTA group were not significantly different from those of the MTA-B group (p > 0.05). The difference of ΔE between the RMTA group after internal bleaching and the RMTA-B group was also not significant (p > 0.05).Conclusions: RetroMTA caused significantly less discoloration than ProRoot MTA. Pre-application of DBA reduced discoloration caused by ProRoot MTA. MTA discoloration was improved equally well between DBA pre-application and post-bleaching.Clinical Relevance: Tooth discoloration by mineral trioxide aggregate (MTA) limits its use in esthetic regions. The pre-application of dentin bonding agent (DBA) and the use of newly developed RetroMTA could help to prevent the discoloration caused by MTA.

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Effect of Dentin Bonding Agent on the Prevention of Tooth Discoloration Produced by Mineral Trioxide Aggregate

International Journal of Dentistry ◽

10.1155/2012/563203 ◽

2012 ◽

Vol 2012 ◽

pp. 1-3 ◽

Cited By ~ 29

Author(s):

Majid Akbari ◽

Armita Rouhani ◽

Sadeq Samiee ◽

Hamid Jafarzadeh

Keyword(s):

Mineral Trioxide Aggregate ◽

Bonding Agent ◽

Color Measurement ◽

Control Group ◽

Dentin Bonding ◽

Tooth Discoloration ◽

Significant Difference ◽

Dentin Bonding Agent ◽

And Control

Objective. Determination of the effect of dentin bonding agent (DBA) on the prevention of tooth discoloration produced by mineral trioxide aggregate (MTA).Methods. 50 teeth were endodontically treated and after removal of 3 mm of obturating materials were divided into five groups. In white MTA (WMTA) and grey MTA (GMTA) groups, these materials were placed in root canal below the orifice. In DBA + WMTA and DBA + GMTA groups, DBAs were applied in the access cavity. Then, 3 mm of WMTA and GMTA was placed. The last 10 teeth served as control. All of teeth were restored and color measurement was recorded for each specimen at this time and 6 months later.Results. The mean tooth discoloration in WMTA and GMTA groups was significantly more than DBA + WMTA and DBA + GMTA groups, respectively. There was no significant difference between DBA + WMTA and DBA + GMTA groups and control group.Conclusion. Application of DBA before MTA may prevent tooth discoloration.

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Pre-application of dentin bonding agent prevents discoloration by mineral trioxide aggregate

10.21203/rs.2.20732/v1 ◽

2020 ◽

Author(s):

Yoo-Lim Choi ◽

Young-Eun Jang ◽

Bom Sahn Kim ◽

Jin-Woo Kim ◽

Yemi Kim

Keyword(s):

Mineral Trioxide Aggregate ◽

Bonding Agent ◽

Control Group ◽

Dentin Bonding ◽

Color Changes ◽

Tooth Discoloration ◽

Significant Difference ◽

Dentin Bonding Agent ◽

The Difference ◽

Root Surfaces

Abstract Background: To evaluate tooth discoloration by newly developed calcium silicate-based materials, and to examine the pre-application of dentin bonding agent (DBA) for preventing discoloration caused by mineral trioxide aggregate (MTA). Methods: The roots of 50 premolars were randomly divided into five groups (n=10) and cavities were prepared from resected root surfaces. MTA was placed in the cavities of teeth belonging to the ProRoot MTA (Pr) and RetroMTA (Rt) groups. For teeth belonging to the DBA + ProRoot MTA (B-Pr) and DBA + RetroMTA (B-Rt) groups, DBA was first applied to the cavities prior to the addition of MTA. Teeth in the control group were restored with composite resin only (i.e., without MTA). After 12 weeks, MTA was removed from the Pr and Rt teeth and bleaching agents were applied for 3 additional weeks. Color assessments were recorded at baseline, and 1, 4, and 12 weeks, as well as after bleaching. Results: Following 12 weeks of MTA treatment, there was a significant difference between the discoloration in the Pr and B-Pr groups. However, no significant difference was observed between the Rt and B-Rt groups. Following bleaching, the color changes (ΔE values) of the Pr group were not significantly different from those of the B-Pr group. The difference of ΔE between the Rt group after internal bleaching and the B-Rt group also was not significant. Conclusions: RetroMTA caused significantly less discoloration than ProRoot MTA. Pre-application of DBA prevented discoloration by ProRoot MTA. MTA discoloration was improved equally well between DBA pre-application and post-bleaching. Clinical Relevance Tooth discoloration by mineral trioxide aggregate (MTA) limits its use in esthetic region. The pre-application of dentin bonding agent (DBA) and the newly developed RetroMTA prevented discoloration caused by MTA, thus expanding its use in esthetic applications.

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