scholarly journals In Vitro Antibacterial Effect of Fifth Generation Dentin Bonding Agent Incorporated With Nisin on Streptococcus Mutans

2021 ◽  
Author(s):  
Gopal Keerthipriya ◽  
Nesamani Ravikumar ◽  
Sekar Mahalaxmi
Keyword(s):  
Streptococcus Mutans ◽  
Optical Density ◽  
Bacterial Growth ◽  
Membrane Integrity ◽  
Bonding Agent ◽  
Control Group ◽  
Integrity Test ◽  
Secondary Caries ◽  
Fifth Generation ◽  
Live Bacteria

Abstract Introduction Secondary caries is the most common cause of failure of composite restorations. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with Nisin, a polypeptide bacteriocin against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the three experimental bonding agents, namely control BA only, bonding agent with 1 wt% Nisin (NBA 1) and 5 wt% Nisin (NBA 5) were obtained using 250 μl Brain Heart Infusion (BHI) broth. To this, 10 μl S. mutans culture was added and incubated at 37°C. Bacterial growth was estimated by changes in optical density using spectrophotometry every 20 min for 2 hours. The results were statistically analysed using one way ANOVA followed by Post Hoc test. For adherence and membrane integrity test, 10μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 hours. After rinsing, 1ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under CLSM for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals, with experimental groups showing concentration dependant reduction in bacterial growth. Correspondingly, the experimental groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent Nisin in bonding agent may render the resin dentin interface more resistant to bacterial penetration, thus reducing the incidence of secondary caries.

scholarly journals In vitro antibacterial effect of fifth generation dentin bonding agent incorporated with nisin on Streptococcus mutans

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Gopal Keerthipriya ◽  
Nesamani Ravikumar ◽  
Sekar Mahalaxmi
Keyword(s):  
Streptococcus Mutans ◽  
Optical Density ◽  
Bacterial Growth ◽  
Membrane Integrity ◽  
Bonding Agent ◽  
Control Group ◽  
Dentin Bonding ◽  
Fifth Generation ◽  
Live Bacteria ◽  
Dentin Bonding Agent

Abstract Introduction Bacterial adherence to restorative materials such as composite resin is one of the aetiology of secondary caries. This study evaluated the antibacterial efficacy of fifth generation bonding agent (BA) modified with nisin, against Streptococcus mutans based on its growth, adherence and membrane integrity. Methods Adhesive eluents of the experimental bonding agents were obtained using 250 μl Brain Heart Infusion (BHI) broth and the groups were control (BA with 0% Nisin), bonding agent with 1 wt% (NBA 1) and 5 wt% nisin (NBA 5). To this, 10 μl S. mutans culture was added and incubated at 37 °C. Bacterial growth was estimated by changes in optical density using spectrophotometer every 20 min for 2 h. The results were statistically analysed using one way ANOVA followed by Tukey Post Hoc test. For adherence and membrane integrity test, 10 μl of BHI supplemented with 1% sucrose and 50 μl of bacterial suspension were inoculated onto the cured specimens, and incubated for 4 h. After rinsing, 1 ml of Live/Dead BacLight bacterial viability stain was added and incubated in the dark for 15 min and observed under confocal laser scanning microscope (CLSM) for intact (green/live) and damaged (red/dead) bacterial membranes. Results Mean optical density was significantly higher in control group at all time intervals with maximum value at 2 h (0.83 ± 0.008), while there was a concentration dependant reduction in bacterial growth with the NBA groups (0.50 ± 0.007). Correspondingly, the NBA groups showed higher amount of dead than live bacteria, while live bacteria were predominant in the control group. Significance Addition of an antibacterial agent nisin in dentin bonding agent may render the resin dentin interface more resistant to bacterial penetration, and adherence of cariogenic bacteria like S. mutans.

scholarly journals Effect of Precuring and Postcuring of Total-etch and Self-etch Bonding Agents on the Microleakage of Fissure Sealants

2020 ◽  
Author(s):  
Bahman Seraj ◽  
Ghasem Meighani ◽  
Shabnam Milani ◽  
Mostafa Fatemi
Keyword(s):  
Pediatric Dentistry ◽  
Bonding Agent ◽  
Control Group ◽  
Single Bond ◽  
Bonding Agents ◽  
Fissure Sealant ◽  
The Third ◽  
Fifth Generation ◽  
Significant Difference ◽  
Self Etch

Objectives: Considering the importance of timesaving in pediatric dentistry, if the efficacy is achieved along with shorter working time and less technical sensitivity, the behavior management of young patients can be anticipated. This study aimed to compare the effect of precuring and postcuring of total-etch and self-etch bonding agents on the microleakage of sealants. Materials and Methods: This study was conducted on forty impacted third molars, which were surgically extracted. The samples were divided into five groups: 1. Control (etching and sealant), 2. Precured fifth-generation bonding agent (Adper Single Bond 2) and fissure sealant, 3. Postcured fifth-generation bonding agent (Adper Single Bond 2) and fissure sealant, 4. Precured seventh-generation bonding agent (Single Bond Universal) and fissure sealant, and 5. Postcured seventh-generation bonding agent (Single Bond Universal) and fissure sealant. All specimens were thermocycled (×1000), immersed in 0.5% basic fuchsine solution for 24 hours, sectioned, digitally photographed, and measured using the LAS EZ program. The data were analyzed using Kruskal-Wallis and Dunn’s tests. Results: Leakage in the control group and the third group was significantly lower than that in other groups. In pairwise comparisons, a significant difference was found between the control group and the fifth group and between the third group and the fifth group. Conclusion: The conventional method of sealant placement showed superior results in comparison with the use of an intermediate layer of the bonding agent.

Antibacterial Effect of Experimental Orthodontic Adhesives Containing Gold Nanoparticles against Streptococcus mutans and Streptococcus sobrinus

2021 ◽  
Vol 904 ◽  
pp. 301-308
Author(s):  
Pinyada Akarajarasrod ◽  
Surachai Dechkunakorn ◽  
Pornpen Tantivitayakul ◽  
Primana Punnakitikashem ◽  
Wassana Wichai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Bacterial Growth ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Control Group ◽  
White Spot Lesion ◽  
Streptococcus Sobrinus ◽  
Orthodontic Adhesives

Enamel decalcification usually can be found around orthodontic bracket after debonding. Orthodontic adhesives resistant to bacterial colonization were used to prevent white spot lesion and dental caries in orthodontic treatment with fixed appliance. The objectives of this study were to evaluate the antibacterial properties of expeimental orthodontic adhesive containing gold nanoparticles (AuNPs). 108 orthodontic adhesive discs containing 1.0 wt%, 0.5 wt%, 0.25 wt% AuNPs and without AuNPs were prepared from in-house orthodontic adhesive (27 discs per group). The antibcterial properties of adhesive discs were evaluated by direct contact test. Streptococcus mutans and Streptococcus sobrinus suspensions were placed on the discs and incubated at 37 °C for 1 hour. The adhesive discs were transferred to BHI broth and were incubated at 37°C for 16, 20 and 24 h. Bacterial growth was evaluated by optical density (OD) measurement at 590nm. The results showed that experimental orthodontic adhesives with 1.0 wt% and 0.5 wt% AuNPs exhibited higher antibacterial activity compared to the control group (p < 0.05). At 24 h of incubation, the median OD of 1.0 wt%, 0.5 wt%, 0.25 wt% and control in S. mutans were 0.109, 0.006, 0.007 and 0.372, rescpectively. In S. sobrinus, the median OD were 0.173, 0.012 , 0.007 and 0.328. The incorporation of gold nanoparticles into orthodontic adhesives exhibited antibacterial activity against cariogenic microorganism. At a concentration of 0.5 wt% and 1.0 wt% AuNPs, experimental orthodontic adhesive could reduce bacterial growth of both S. mutans and S. sobrinus.

Effects of High Pressure Processing on the Quality of Vacuum Packed Fish Patties

2014 ◽  
Vol 644-650 ◽  
pp. 4671-4676
Author(s):  
Ying Chun Zhu ◽  
Li Zhen Ma ◽  
Yu Jing Tian ◽  
Hua Yang ◽  
Yao Hua Guo ◽  
...  
Keyword(s):  
High Pressure ◽  
Bacterial Growth ◽  
Reduction Potential ◽  
Meat Products ◽  
High Pressure Processing ◽  
Control Group ◽  
Oxidation Reduction ◽  
Preservation Method ◽  
Oxidation Reduction Potential

The objective of this study was to evaluate the use of high pressure processing (HPP) as a preservation method of meat products. Vacuum-packaged fish patties were subjected to HPP (300 MPa for 30 min 15°C or 500 Mpa for 10 min at 15°C). Untreated samples represented the control group. The three groups were stored at 4°C for 0–5 weeks. Color parameters, pH, thiobarbituric (TBARS), bacterial growth, and Oxidation-Reduction Potential (ORP) were determined. The results revealed that the 500-MPa treatment inhibited bacterial growth and extended the shelf-life of fish patties to four weeks with insignificant effects on the physicochemical attributes.

scholarly journals Antibiofilm Activity of Mangosteen (Garcinia mangostana L.) Flavonoids against Streptococcus mutans Bacteria

2020 ◽  
Vol 10 (2) ◽  
pp. 48
Author(s):  
Sri Kunarti ◽  
Aulia Ramadhani ◽  
Laskmiari Setyowati
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Tooth Enamel ◽  
Tooth Surface ◽  
Control Group ◽  
Antibiofilm Activity ◽  
Garcinia Mangostana ◽  
Control Group Design ◽  
Biofilm Inhibition ◽  
Significant Difference

Background: Dental caries is one of the most common infectious diseases and often occurs in the community caused by bacteria. Attached bacteria in the tooth surface for a long time will form a biofilm and will lead to demineralization characterized by damage in the structure of the tooth enamel. The bacteria that cause dental caries and can form biofilms is Streptococcus mutans. The bacteria inside biofilms are more resistant to antibacterial agents. Flavonoids in mangosteen pericarp extract can be a cleaner alternative for the anti-biofilm cavity that has properties against Streptococcus mutans. Purpose: To determine the activity of flavonoids in mangosteen pericarp extract at a certain concentration against Streptococcus mutans bacteria. Methods: This study was a laboratory experimental study with a post-test only control group design. Streptococcus mutans were diluted according to the Mc Farland dilution standard 106 in Tryptic Soy Broth (TSB) medium and put in a flexible U-bottom microtiter plate. Then it was incubated for 5x24 hours and checked using crystal violet simple staining to see the formation of biofilms. Flavonoid extract of mangosteen pericarp performed serial dilution in a concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78% was added, and the incubation process were conducted for 1x24 hours. OD (Optical Density) readings were done with a wavelength of 595 nm. Results: There was a significant difference between the test groups and the positive control group. The concentration of 100% had the anti-biofilm activity and showed the value of the highest percentage of inhibition, whilst the concentration of 0.78% showed a minimum biofilm inhibition concentration. The results were demonstrated by a statistical analysis test. Conclusion: Flavonoid extract of mangosteen pericarp at a certain concentration has anti-biofilm activity against Streptococcus mutans biofilm.

scholarly journals Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

2020 ◽  
Vol 8 (3) ◽  
pp. 801
Author(s):  
Damajanty H. C. Pangemanan ◽  
Christy Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Streptococcus Mutans ◽  
Soft Tissues ◽  
Inhibitory Concentration ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Alternative Medicines ◽  
Pharmaceutical Properties

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

scholarly journals THE IMPACT OF ASTHMAAND ITS MEDICATIONS ON DENTAL CARIES STATUS AND STREPTOCOCCUS MUTANS COUNT IN CHILDREN: A CROSS-SECTIONAL STUDY

2020 ◽  
pp. 20-23
Author(s):  
Sneha Upadhyay ◽  
Jyoti Bhavthankar ◽  
Mandakini Mandale ◽  
Nivedita Kaorey
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Cross Sectional Study ◽  
Control Group ◽  
Healthy Children ◽  
Sectional Study ◽  
Cross Sectional ◽  
Asthmatic Children ◽  
Significant Difference ◽  
The Impact

Background: Asthma and its medications have been linked to oral diseases in asthmatic children. Aim: Assessment of the dental caries status, salivary Streptococcus mutans count and S. mutans colony score in children receiving inhaled anti asthmatic medications and their comparison in healthy children Material and Method: A cross-sectional study was performed on 40 asthmatic children and 40 healthy children in the age group of 6-14 years. DMFT/deft indices were calculated and saliva samples were collected. Diluted saliva was inoculated on MSB agar plates. S. mutans count and colony score were analysed after 24-48 hours of inoculation. Results: Statistically significant difference was observed in the mean DMFT/deft index, salivary S. mutans load and S. mutans colony score in children of the asthmatic group and control group. Conclusion: Prevalence of dental caries and cariogenic bacteria is higher in asthmatic children.

scholarly journals Phaeodactylum Trıcornutum Kültürlerinde Biyomas Tahmini

2017 ◽  
Vol 5 (2) ◽  
pp. 182
Author(s):  
Burcu Ak ◽  
Gökhan Tamer Kayaalp ◽  
Oya Işık ◽  
Melis Çelik Güney
Keyword(s):  
Light Intensity ◽  
Chlorophyll A ◽  
Optical Density ◽  
Phaeodactylum Tricornutum ◽  
Dry Matter ◽  
Nitrogen Deficiency ◽  
Regression Equation ◽  
Control Group ◽  
Analysis Method ◽  
The Mathematical Model

Phaeodactylum tricornutum is the microalgae that is known to produce lipid. In this study, it was aimed to estimate the microalgae Phaeodactylum tricornutum dry matter cultured in the conditions of nitrogen deficiency as a stress factor, in outdoor, in photo bioreactors, by using multiple regression analysis method. In this study, diatom Phaeodactylum tricornutum (Bohlin) was cultured medium of which N was reduced by 50% and with 20% inoculation ratio. The light intensity, temperature, optical density and chlorophyll a, were measured daily. The mathematical model was formed for control group and 50 % of the N applied to the group using optical density, temperature, light intensity, chlorophyll a and without chlorophyll a. The regression equation of control group was estimated. The R2 value of control group was found 95.1% and statistically significant. Then, the regression equation was estimated for control group without chlorophyll a. The R2 value of this equation was found 94.0% and statistically significant. And then, the regression equation was estimated in 50% nitrogen. The R2 value of control group was found 92.4% and statistically significant. Finally, the regression equation was estimated in 50% nitrogen deficiency without chlorophyll a. The R2 value of this equation was found 91.7% and statistically significant.

scholarly journals Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by1H NMR

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Halyna Hrynash ◽  
Vinay Kumar Pilly ◽  
Alexandra Mankovskaia ◽  
Yaoyang Xiong ◽  
Getulio Nogueira Filho ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Inhibition ◽  
Bacterial Growth ◽  
Testing Machine ◽  
Control Group ◽  
Uv Spectrophotometry ◽  
Release Rates ◽  
H Nmr ◽  
Colony Forming Units ◽  
Bacterial Growth Inhibition

Objective.To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY;Vaccinium macrocarpon).Methods.Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture (Streptococcus mutans) for 24 h. Cell viability (n=3) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus (E) were tested on a universal testing machine (n=8). Compound release and chemical stability were evaluated by UV spectrophotometry and1H NMR (n=3). Data were analyzed by one-way ANOVA and Tukey’s test (α= 0.05).Results.Both compounds inhibited S. mutans growth, with CHX being most effective (P<0.05). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P<0.05). The 24 h compound release rates were ACY: 1.33 μg/mL and CHX: 1.92 μg/mL.1H NMR spectra suggests that both compounds remained stable after being released in water.Conclusion.The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.

Diagnosis of the Syndrome of Excessive Bacterial Growth of the Small Intestine in Patients with Bauhinia Insufficiency and After its Surgical Correction (First Results)

2020 ◽  
pp. 1-6
Author(s):  
Vladimir Leonidovich Martynov ◽  
◽  
Natalia Vladimirovna Kazarina ◽  
Keyword(s):  
Small Intestine ◽  
Bacterial Growth ◽  
Ileocecal Valve ◽  
Normal Function ◽  
Main Group ◽  
Surgical Correction ◽  
Control Group ◽  
Distal Small Intestine ◽  
First Results ◽  
Respiratory Test

Objective: To determine the syndrome of excessive bacterial growth in the small intestine (SIBO) in patients with insufficiency of the bauhinia valve (NBZ) and after its surgical correction. Methods of examination: Patients of the studied groups were examined using direct and indirect methods of diagnosis of SIBO. Bacteriological examination of ileal aspirate and other surgical material was performed. All patients underwent a hydrogen respiratory test with a load of lactulose and performed a qualitative reaction of urine to indican. Characteristics of the material: 50 patients were examined, of which 30 were included in the main group, who underwent surgical correction of NBZ - Bauginoplasty; 20 patients are included in the control group in which the ileocecal valve is consistent. Patients of the main group were examined before surgery and on the 7th and 45th day after Bauhinoplasty. Main results: All patients of the main group had SIBS of varying severity, in 80% of patients SIBS was localized in the distal small intestine. Patients with normal function of ileocecal valve syndrome of excessive bacterial growth did not suffer. Intraoperatively, 76% of patients showed signs of mesenteric mesentery of the small intestine, and the fact of bacterial translocation in SIBO was also confirmed. After 7 days after surgical correction of the Bauhinia valve, the normalization of peak and background excretion of hydrogen was observed in 37% of patients. For 45 days in all patients the hydrogen curve corresponded to the norm. Conclusions: the deficiency of the bauhinia valve is obligately accompanied by the syndrome of excessive bacterial growth in the small intestine, surgical correction is an effective method for correcting the syndrome of excessive bacterial growth in patients with NBD.

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