Pathogenicity induced by indigenous Beauveria bassiana isolate in different life stages of the cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) under laboratory conditions

Abstract Background The invincible effects of the entomopathogenic fungi (EPF) under appropriate circumstances compensate for the flaws of chemical insecticides in the control programs. Beauveria bassiana is one of the most elaborated EPF of pest control all over the world. The potential of using the B. bassiana isolate (Y-F_ITS1) was examined against different larval instars (L2, L3, L4, and L5), pupae, and eggs of the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), using different concentrations under laboratory conditions. Results In bioassay, 2nd instar larvae of S. littoralis were significantly the most susceptible stage to B. bassiana isolate at P ≤ 0.05. It is apparent from the results that the cumulative mortality percentage increased with elapsing time with typical symptoms of infection and sporulation. High efficacies ranging from 85.0 to 99.0% corrected mortality rates were recorded for 2nd instar larvae at the spore concentration (1 × 109 spores ml−1). The mortality percentage reached 90.0% for the 3rd instar larvae treated with the same concentration after 5 days. The least mortality rates results were recorded at the 5th instar larvae. B. bassiana isolate showed an ovicidal effect to the eggs of S. littoralis that reached 100% mortality when treated with 1.0 × 108 and 1.0 × 109 spores ml−1, while the concentration 1 × 107 and 1.0 × 108 caused 65.0 and 87.0%, respectively. Reduction of adult emergence reached (0%) in case of treatment with the highest concentrations (1 × 108 and 1 × 109 spores ml−1). Morphogenetic abnormalities were also recorded. Conclusion Results of mortality rates and lethal concentration values resulted from the experiments indicated that the tested Y-F_ITS1 isolate was efficient and can be recommended as a potential biocontrol agent against S. littoralis. Further field evaluations are still needed.

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Selection of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. for the control of the mite Mononychellus tanajoa (Bondar)

Scientia Agricola ◽

10.1590/s0103-90162004000600015 ◽

2004 ◽

Vol 61 (6) ◽

pp. 659-664 ◽

Cited By ~ 8

Author(s):

Rodrigo Soares Barreto ◽

Edmilson Jacinto Marques ◽

Manoel Guedes Corrêa Gondim Jr. ◽

José Vargas de Oliveira

Keyword(s):

Biological Control ◽

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Total Mortality ◽

Mortality Rates ◽

Mononychellus Tanajoa ◽

Control Programs ◽

Green Mite ◽

Mortality Percentage ◽

Selection Of

The green mite, Mononychellus tanajoa (Bondar) (Acari: Tetranychidae), is considered to be one of the key pests in cassava, Manihot esculenta Crants, leading to considerable field losses. In this study, ten Beauveria bassiana (Bals.) Vuill. and ten Metarhizium anisopliae (Metsch.) Sorok. isolates were evaluated with regard to their potential as biological control agents against adult M. tanajoa females. The total mortality percentage of M. tanajoa caused by B. bassiana ranged from 13.0 to 97.0%, with confirmed mortality rates extending from 9.0 to 91.0% and LT50 varying from 4.2 to 17.0 days. The M. anisopliae isolates showed total mortality percentages ranging from 12.0 to 45.0% with confirmed mortality rates extending from 8.0 to 45.0%, and LT50 varying from 8.6 to 19.8 days. Lethal Concentrations (LC50) of 3.93 × 10(6) conidia mL-1 and 7.44 × 10(8) conidia mL-1 were determined for B. bassiana and M. anisopliae, respectively. B. bassiana isolate 645 was the most efficient, being an alternative for use in biological control programs against the cassava green mite.

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Efficacy of the entomopathogenic fungus, Metarhizium anisopliae (Metsch.), against larvae of the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), under laboratory conditions

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-019-0156-2 ◽

2019 ◽

Vol 29 (1) ◽

Cited By ~ 2

Author(s):

Monir M. M. El Husseini

Keyword(s):

Metarhizium Anisopliae ◽

Entomopathogenic Fungus ◽

Spodoptera Littoralis ◽

Cotton Leafworm ◽

Laboratory Conditions ◽

Lepidoptera Noctuidae

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Susceptibility of field and laboratory strains of Cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) to spinosad pesticide under laboratory conditions

Plant Protection Science ◽

10.17221/5/2015-pps ◽

2016 ◽

Vol 52 (No. 2) ◽

pp. 128-133 ◽

Cited By ~ 3

Author(s):

M.A.I. Ahmed ◽

S.A.H. Temerak ◽

F.A.-K. Abdel-Galil ◽

S.H.M. Manna

Keyword(s):

Spodoptera Littoralis ◽

Cotton Leafworm ◽

Laboratory Conditions ◽

Lepidoptera Noctuidae

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Efficacy of the parasitoid, Trichogrammatoidea bactrae Nagaraja (Hymenoptera: Trichogrammatidae) on the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) egg masses

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00363-z ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Hend O. Mohamed

Keyword(s):

Spodoptera Littoralis ◽

Insect Pests ◽

Single Layer ◽

Control Agent ◽

Physical Characteristics ◽

Great Ability ◽

Egg Masses ◽

Cotton Leafworm ◽

Lepidoptera Noctuidae ◽

Trichogrammatoidea Bactrae

Abstract Background The Egyptian cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the major insect pests, causing a significant damage on different cultivated agricultural crops. Developing an alternative non-chemical tool, an effective and environmentally friendly method to suppress pest's infestation is essentially needed. Therefore, biological control by releasing the egg parasitoids could be the most promising tool for integrated pest management. Results This study was designed to evaluate the efficacy of the egg parasitoid, Trichogrammatoidea bactrae Nagaraja (Hymenoptera: Trichogrammatidae) as a bio-control agent against S. littoralis egg masses with different physical characteristics (number of egg layer and degree of scale density) in a no-choice and choice tests, under laboratory conditions. Also, the parasitoids’ fitness in terms of parasitism percentage, developmental period, adults’ emergence percentage, female offspring percentage, and longevity were investigated. The results revealed that T. bactrae wasps had a great ability to parasitize S. littoralis egg masses, but with different rates, related to their layers and scales’ thickness in both tests. The highest parasitism percentage was observed on one-layer eggs, followed by two layers. However, 3-layer eggs were the least preferable one. High numbers of adult emergencies (> 80%) were observed in all tested egg masses, except in the case of 3 layers with high scales. Furthermore, female-biased sex ratios were noticed at all examined eggs, with only the exception of high-scaly eggs with a single layer that recorded the lowest rate (≤ 45%). Besides, the survival of adult female parasitoids was not significantly affected in both tests. Conclusions T. bactrae could be used as a bio-control agent against S. littoralis egg masses with different physical characteristics based on the achieved results.

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