Effect of Glucose Concentration on Formation of AGEs in Erythrocytesin Vitro

High glucose concentrations (55 mM) were found to suppress the isometric contraction tension of rat diaphragm. The effect appears to be due to the resulting transmembrane osmotic gradient in spite of the fact that glucose uptake by the diaphragm is fairly rapid. Insulin can reverse the effect of hypertonic glucose to a considerable extent. The problems of estimating intracellular glucose concentration in the isolated muscle are considered. Estimates of intracellular glucose taking into account extracellular space and extracellular diffusion gradients were made using a two-compartment model. Calculations based on the model show that in the presence of insulin, intracellular glucose increases from about 1 to 19 μmol/ml of muscle fibre. The mean transmembrane glucose concentration gradient decreases from 41 to 18 μmol/ml. With the use of the model it appears possible to relate most of the observed effect of glucose and insulin on isometric contraction to their influence on the osmotic gradient across the muscle fibre membrane. Insulin appears to have some additional effects on muscles suppressed by hypertonic solutions which are not accounted for by this mechanism.

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The effect of glucose concentration and sodium phenylbutyrate treatment on mitochondrial bioenergetics and ER stress in 3T3-L1 adipocytes

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/j.bbamcr.2014.10.012 ◽

2015 ◽

Vol 1853 (1) ◽

pp. 213-221 ◽

Cited By ~ 14

Author(s):

Ross M. Tanis ◽

Gerardo G. Piroli ◽

Stani D. Day ◽

Norma Frizzell

Keyword(s):

Er Stress ◽

Glucose Concentration ◽

Mitochondrial Bioenergetics ◽

Effect Of Glucose ◽

Sodium Phenylbutyrate

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Glucose Interference in the Pancreolauryl Test

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329803500212 ◽

1998 ◽

Vol 35 (2) ◽

pp. 274-278

Author(s):

A L Trewick

Keyword(s):

Glucose Concentration ◽

Before And After ◽

Effect Of Glucose ◽

Pancreolauryl Test

The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines ( n = 5) were assayed at 60° and 70°C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient urines ( n = 10). Aqueous glucose solutions and spiked (5% glucose) normal random urines ( n = 5) were assayed to assess the effect of glucose concentration alone. There was no difference in T/K ratios for glucose-free patient samples at 60°, 70° and 80°C. After spiking with glucose T/K ratios were significantly different when assayed at 70°C, but not when assayed at 60°C. Aqueous glucose solutions ≥ 0.6% produced a pigment at temperatures ≥ 65°C which absorbed at the λmax of fluorescein. Glucose was found to interfere in the pancreolauryl test. Caution should be exercised when interpreting results from glucosuric samples.

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