Detection of breast cancer metastasis in sentinel lymph nodes using intra-operative real time PCR assay

2008 ◽  
Vol 26 (15_suppl) ◽  
pp. 11050-11050
Author(s):  
R. E. Mansel Professor ◽  
A. Goyal ◽  
A. Douglas-Jones ◽  
V. Woods ◽  
B. Jasani
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Real Time ◽  
Real Time Pcr ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Sentinel Lymph Nodes ◽  
Pcr Assay

scholarly journals Mammaglobin RT-qPCR for breast cancer metastasis detection in sentinel lymph nodes

2010 ◽  
Vol 101 (11) ◽  
pp. 2501-2501 ◽  
Author(s):  
Paul N. Span ◽  
Hanneke W. Van Laarhoven ◽  
Fred C. G. J. Sweep
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Sentinel Lymph Nodes

Reply to Span et al.: Mammaglobin RT-qPCR for breast cancer metastasis detection in sentinel lymph nodes

2010 ◽  
Vol 101 (11) ◽  
pp. 2502-2502
Author(s):  
Yan-Hui Liu ◽  
Fang-Ping Xu ◽  
Ning Liao ◽  
Li Li ◽  
Guo-chun Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Sentinel Lymph Nodes

scholarly journals Genetic Heterogeneity of Breast Cancer Metastasis May Be Related to miR-21 Regulation of TIMP-3 in Translation

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jianyi Li ◽  
Yang Zhang ◽  
Wenhai Zhang ◽  
Shi Jia ◽  
Rui Tian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Noncoding Rna ◽  
Cancer Metastasis ◽  
Inverse Correlation ◽  
Breast Cancer Metastasis ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Cancer Proliferation ◽  
Rna Molecules ◽  
Polymerase Chain

Purpose.MicroRNAs are noncoding RNA molecules that posttranscriptionally regulated expression of target gene and implicate the progress of cancer proliferation, differentiation, and apoptosis. The aim of this study is to determine whether microRNA-21 (miR-21), a specific microRNA implicated in multiple aspects of carcinogenesis, promoted breast cancer metastasis by regulating the tissue inhibitor of metalloproteinase 3 (TIMP-3) gene.Methods.miR-21 of serum and tissue from 40 patients (30 patients with breast cancer) were detected by real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). TIMP-3 of tissue from the patient was tested by real-time RT-qPCR. Protein expression of TIMP-3 was evaluated by western blotting. Correlation analysis was performed between miR-21 and TIMP-3.Results.Of the 40 samples from tissue and serum analyzed, the miR-21 expression was significantly higher in high invasion metastasis group (HIMG) that in low invasion metastasis group (LIMG); the latter was higher than that in normal group (NG). Additionally, the TIMP-3 expression was significantly lower in HIMG than in LIMG; the latter was lower than that in NG. There was significantly inverse correlation between miR-21 and TIMP-3 extracted from tissue.Conclusion.Our data suggest that miR-21 could promote metastasis in breast cancer via the regulation of TIMP3 translation, and there was consistency between miR-21 of serum and miR-21 in tissue.

scholarly journals Detection of Circulating Tumor Cells in Early-Stage Breast Cancer Metastasis to Axillary Lymph Nodes

2007 ◽  
Vol 13 (14) ◽  
pp. 4105-4110 ◽  
Author(s):  
Taku Nakagawa ◽  
Steve R. Martinez ◽  
Yasufumi Goto ◽  
Kazuo Koyanagi ◽  
Minoru Kitago ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metastasis ◽  
Early Stage ◽  
Breast Cancer Metastasis ◽  
Early Stage Breast Cancer ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph
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