Cytoplasmic expression of HuR and cyclooxygenase-2 expression in colon cancer

e22161 Background: HuR, human family embryonic-lethal abnormal vision-like protein, can bind to mRNAs and stabilizes them in the cytoplasm, resulting in more efficient translation. HuR is predominantly present in the nucleus and shuttles between the nucleus and cytoplasm. The mRNA of cyclooxygenase-2 (Cox-2) is stabilized by HuR in several cancers, including breast, stomach, lung and brain. Methods: We investigated the expression and its cellular location of HuR, and Cox-2 expression in 79 colorectal cancer patients with immunohistochemistry, and evaluated the biological implications in colorectal carcinoma. Results: Nuclear HuR expression was observed in 59 (74.7%) and cytoplasmic HuR expression was seen in 25 (31.6%). Cox-2 immunoreactivity was noted in 42 (53%). The expression of cytoplasmic HuR was significantly associated with Cox-2 expression (p=0.004). And cytoplasmic expression of HuR showed correlation with lymphatic invasion (p=0.025) and lymph node metastasis (p=0.027). Nuclear HuR showed no correlation with Cox-2 expression or other clinicopathological parameters examined. Conclusions: These results suggest that cytoplasmic translocation of HuR is associated with Cox-2 expression in some colorectal carcinoma. No significant financial relationships to disclose.

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Expression of Embryonic Lethal Abnormal Vision (ELAV)-Like Protein HuR and Cyclooxygenase-2 (COX-2) in Ewing Sarcoma

Tumori Journal ◽

10.1177/030089160809400310 ◽

2008 ◽

Vol 94 (3) ◽

pp. 347-350 ◽

Cited By ~ 8

Author(s):

Sung-Im Do ◽

Eduard Santini Araujo ◽

Ricardo K Kalil ◽

Patrizia Bacchini ◽

Franco Bertoni ◽

...

Keyword(s):

Ewing Sarcoma ◽

Cyclooxygenase 2 ◽

Embryonic Lethal ◽

Cox 2

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Overexpression of cyclooxygenase-2 (COX-2) is an independent predictive factor of poor prognosis in patients with stage II sporadic colorectal carcinoma

Gastroenterology ◽

10.1016/s0016-5085(03)80322-0 ◽

2003 ◽

Vol 124 (4) ◽

pp. A65

Author(s):

Buecher Bruno ◽

Lievre Astrid ◽

Heymann Marie-Francoise ◽

Bezieau Stephane ◽

Mosnier Jean-Francois ◽

...

Keyword(s):

Colorectal Carcinoma ◽

Predictive Factor ◽

Poor Prognosis ◽

Cyclooxygenase 2 ◽

Stage Ii ◽

Independent Predictive Factor ◽

Sporadic Colorectal Carcinoma ◽

Cox 2

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Immunohistochemical Expression of “HCG-β” in Colorectal Carcinoma

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6854 ◽

2021 ◽

Vol 9 (A) ◽

pp. 789-797

Author(s):

Amira Mohamed Bassam ◽

Yousra Raafat ◽

Ahmed Mahmoud Abd Al-Aziz ◽

Rasha Ramadan Mostafa

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Colorectal Carcinoma ◽

Tumor Invasion ◽

Lymphovascular Invasion ◽

Histological Grade ◽

Signet Ring Cell ◽

Tumor Budding ◽

Clinicopathological Parameters ◽

Node Metastasis

BACKGROUND: Tumor budding is associated with adverse histology and is a predictor of lymph node metastasis. Human chorionic gonadotropin-beta (hCG-β) expression in non-trophoblastic tumors has been associated with aggressive behavior. AIM: Evaluation of tumor budding and hCG-β _immunohistochemical expression in colorectal carcinoma (CRC), and correlation of their expression with various clinicopathological parameters. MATERIALS AND METHODS: Immunohistochemical staining for hCG-β _was performed on paraffin-embedded sections of 60 cases of CRC. Tumors with cytoplasmic or membranous staining of more than five epithelial cell clusters were designated hCG-β _positive; otherwise, they were designated hCG-β _negative. Tumor budding was assessed in hematoxylin and eosin stained slides and was classified as; low: 0–4 buds, intermediate: 5–9 buds and high: ≥10 buds; with exclusion of pure mucoid or signet ring cell morphology cases from analysis. RESULTS: Tumor budding was low in (58.8%) of the cases, intermediate in (15.7%), and high in (25.5%). There was a statistically significant correlation between tumor budding and tumor histological grade (p = 0.011), lymph node metastasis (N) (p = 0.009), overall pathologic stage group (p = 0.009), modified Dukes’ stage (p = 0.009), lymphovascular invasion (p = 0.000), and desmoplastic reaction (p = 0.004). Positive hCG-β _alpha expression was detected in 12 (20%) of cases. There were statistically significant correlations between hCG-β _expression and each of lymphovascular invasion (p = 0.042) and tumor budding (p = 0.000). CONCLUSION: hCG-β _is a marker of aggressiveness that may have essential role in tumor invasion. Tumor budding is crucial event in tumor invasion and metastasis. Tumor budding with hCG-β _expression is a novel prognostic parameter and may represent a potential therapeutic target.

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A cyclooxygenase-2 (COX-2) selective non-steroidal anti-inflammatory drug enhances the growth inhibitory effect of butyrate in colorectal carcinoma cells expressing COX-2 protein: regulation of COX-2 by butyrate

Carcinogenesis ◽

10.1093/carcin/21.1.69 ◽

2000 ◽

Vol 21 (1) ◽

pp. 69-77 ◽

Cited By ~ 44

Author(s):

T. E. Crew

Keyword(s):

Colorectal Carcinoma ◽

Inhibitory Effect ◽

Cyclooxygenase 2 ◽

Carcinoma Cells ◽

Protein Regulation ◽

Growth Inhibitory Effect ◽

Inflammatory Drug ◽

Growth Inhibitory ◽

Cox 2 ◽

Anti Inflammatory

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Expression of Beta-Catenin in Colorectal Carcinoma and its Association with Various Clinicopathological Parameters

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/48129.15151 ◽

2021 ◽

Author(s):

Monika Panda ◽

Ranjita Panigrahi ◽

Goutami Das Nayak ◽

Urmila Senapati ◽

Saroj Ranjan Sahoo ◽

...

Keyword(s):

Colorectal Carcinoma ◽

Malignant Lesion ◽

Clinicopathological Parameters ◽

Gradual Transition ◽

Histopathological Study ◽

Malignant Neoplasms ◽

Beta Catenin ◽

Benign Lesions ◽

Cytoplasmic Expression ◽

Malignant Lesions

Introduction: Colorectal Carcinoma (CRC) is the third most commonly diagnosed cancer and the second leading cause of cancer related mortality globally. It is a source of concern for researchers worldwide and hence, a lot of emphasis is being given towards early detection and targeted drug therapy to improve the survival rate. Aim: To study the expression of beta-catenin in colonic polyps, adenomas and CRC and to associate beta-catenin expression with various clinicopathological features. Materials and Methods: This was a prospective cross-sectional study conducted in the Department of Pathology, Kalinga Institute of Medical Sciences, Bhubaneswar, Odisha, India from September 2018 to August 2020. Colonoscopic biopsies, mucinous carcinoma and poorly preserved tissue were excluded. Histopathological study and Immunohistochemistry evaluation of beta-catenin was done. Statistical analysis was done by using appropriate tests. A p-value of less than 0.05 was taken as statistically significant. Results: Out of 80 cases, 40 cases were benign lesions Non neoplastic polyp and adenoma) and 40 cases were adenocarcinoma. It was observed that benign lesions had maximum cases with preserved membranous expression (36/40) and very few cases (4/40) showed cytoplasmic expression of beta-catenin. But in carcinoma, high cytoplasmic expression was seen in 20/40 (50%) whereas 8/40 (20%) cases had nuclear positivity. Membranous beta-catenin expression was significantly higher in benign lesions than in the malignant lesions (IS:8.75±3.09 versus 4.30±2.70) respectively; (p<0.0001). But cytoplasmic beta-catenin expression was low in benign lesion as compared to malignant lesion (IS: 2.07±3.46 versus 5.35±3.14), respectively; (p<0.0001). However, nuclear beta-catenin expression was extremely low in benign lesions than in malignant lesions (0.08±0.47 versus 1.90±3.49), respectively; (p=0.0016), this difference was statistically significant. Conclusion: The present study demonstrates the change in beta-catenin expression with gradual transition from predominantly membranous pattern to cytoplasmic or nuclear as we progress from normal colorectal tissues to polyps, benign premalignant lesions and malignant neoplasms. This property of beta-catenin helps in determining malignant potential of various premalignant neoplasms of large intestine which in turn helps in initiating early prophylactic treatment.

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