Evalution of effect of Vitamin D levels and tumor Vitamin D receptor status as a risk factor and marker for therapeutic response Indian breast cancer patients.

There is a mushrooming interest in the anti-carcinogenic property of vitamin D. However, many researches reported a conflicting result in the association of vitamin D levels to certain types of cancer. This study was designed to assess the association between vitamin D and vitamin D receptor (VDR) expression with breast cancer. This case-control study, carried out at Hospital Universiti Sains Malaysia, Kelantan, involved 69 newly diagnosed breast cancer patients and 73 healthy volunteers. Serum 25(OH)D was taken and compared between 2 groups. VDR expression in patients’ breast tissue samples was determined by immunohistochemical staining method using anti-VDR antibody. 85.5% of breast cancer patients and 97.3% of healthy control were vitamin D insufficient with a mean (SD) of 13.36 (6.96) ng/mL and 13.05 (3.71) ng/mL, respectively, and the difference was not statistically significant. VDR expression showed cytoplasmic positivity in 75.4% of breast cancer tissue, followed by both cytoplasmic and nuclear positivity in 21.5% and complete absence in 3%. There was no significant association between VDR expression and hormone receptor status. In conclusion, there was a high prevalence of vitamin D deficiency among breast cancer and healthy volunteers in our study. There was no significant association between breast cancer and vitamin D. The VDR expression in breast cancer cells showed high cytoplasmic localization.

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Circulating Vitamin D and Overall Survival in Breast Cancer Patients: A Dose-Response Meta-Analysis of Cohort Studies

Integrative Cancer Therapies ◽

10.1177/1534735417712007 ◽

2017 ◽

Vol 17 (2) ◽

pp. 217-225 ◽

Cited By ~ 20

Author(s):

Kejia Hu ◽

David Frederick Callen ◽

Jiayuan Li ◽

Hong Zheng

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Overall Survival ◽

Cancer Patients ◽

Dose Response ◽

Fixed Effects ◽

Meta Analysis ◽

Breast Cancer Patients ◽

Fixed Effects Model ◽

Vitamin D Levels

Studies have shown that vitamin D could have a role in breast cancer survival; however, the evidence of the relationship between patients’ vitamin D levels and their survival has been inconsistent. This meta-analysis explores possible dose-response relationships between vitamin D levels and overall survival by allowing for differences in vitamin D levels among populations of the various studies. Studies relating vitamin D (25-OH-D [25-hydroxyvitamin D]) levels in breast cancer patients with their survival were identified by searching PubMed and Embase. A pooled HR (hazard ratio) comparing the highest with the lowest category of circulating 25-OH-D levels were synthesized using the Mantel-Haenszel method under a fixed-effects model. A two-stage fixed-effects dose-response model including both linear (a log-linear dose-response regression) and nonlinear (a restricted cubic spline regression) models were used to further explore possible dose-response relationships. Six studies with a total number of 5984 patients were identified. A pooled HR comparing the highest with the lowest category of circulating 25-OH-D levels under a fixed-effects model was 0.67 (95% confidence interval = 0.56-0.79, P < .001). Utilizing a dose-response meta-analysis, the pooled HR for overall survival in breast cancer patients was 0.994 (per 1 nmol/L), Pfor linear trend < .001. At or above a 23.3 nmol/L threshold, for a 10 nmol/L, 20 nmol/L, or 25 nmol/L increment in circulating 25-OH-D levels, the risk of breast cancer overall mortality decreased by 6%, 12%, and 14%, respectively. There was no significant nonlinearity in the relationship between overall survival and circulating 25-OH-D levels. Our findings suggest that there is a highly significant linear dose-response relationship between circulating 25-OH-D levels and overall survival in patients with breast cancer. However, better designed prospective cohort studies and clinical trials are needed to further confirm these findings.

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Vitamin D receptor (VDR) gene polymorphism BsmI in Syrian breast cancer patients

Meta Gene ◽

10.1016/j.mgene.2020.100766 ◽

2020 ◽

Vol 26 ◽

pp. 100766

Author(s):

Shaden Haddad

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Gene Polymorphism ◽

Cancer Patients ◽

Vitamin D Receptor ◽

Breast Cancer Patients ◽

Vdr Gene ◽

Vdr Gene Polymorphism

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Vitamin D levels in Mediterranean breast cancer patients compared with those in healthy women

Maturitas ◽

10.1016/j.maturitas.2018.07.015 ◽

2018 ◽

Vol 116 ◽

pp. 83-88 ◽

Cited By ~ 1

Author(s):

Marta Pineda-Moncusí ◽

Miguel Angel Garcia-Perez ◽

Abora Rial ◽

Guillem Casamayor ◽

Maria Lourdes Cos ◽

...

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Cancer Patients ◽

Breast Cancer Patients ◽

Healthy Women ◽

Vitamin D Levels

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Prognostic characteristics of 492 newly diagnosed breast cancer patients by serum vitamin D levels.

Journal of Clinical Oncology ◽

10.1200/jco.2015.33.15_suppl.e12619 ◽

2015 ◽

Vol 33 (15_suppl) ◽

pp. e12619-e12619 ◽

Cited By ~ 1

Author(s):

Luke Joseph Peppone ◽

Mohamedtaki Abdulaziz Tejani ◽

Karen Michelle Mustian ◽

Michelle Christine Janelsins ◽

Charles Stewart Kamen ◽

...

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Cancer Patients ◽

Serum Vitamin ◽

Newly Diagnosed ◽

Breast Cancer Patients ◽

Serum Vitamin D ◽

Vitamin D Levels

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Haplotype Analysis of Vitamin D Receptor (VDR) Gene in Breast Cancer Patients

Clinical Laboratory ◽

10.7754/clin.lab.2020.200931 ◽

2021 ◽

Vol 67 (06/2021) ◽

Author(s):

Emel Ergul ◽

Turgay Simsek ◽

Nihal Uren ◽

Tugcan Korak ◽

Hayal Simsek ◽

...

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Cancer Patients ◽

Vitamin D Receptor ◽

Haplotype Analysis ◽

Breast Cancer Patients ◽

Vdr Gene

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Abstract 3286: Correlation between vitamin D levels and DNA repair capacity in breast cancer patients stratified by molecular subtypes

10.1158/1538-7445.am2017-3286 ◽

2017 ◽

Author(s):

Carmen Ortiz ◽

Jarline Encarnación ◽

Ralphdy Vergne ◽

Wanda Vargas ◽

Jaime Matta

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Dna Repair ◽

Cancer Patients ◽

Molecular Subtypes ◽

Breast Cancer Patients ◽

Repair Capacity ◽

Dna Repair Capacity ◽

Vitamin D Levels

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Inhibition of Antiestrogen-Promoted Pro-Survival Autophagy and Tamoxifen Resistance in Breast Cancer through Vitamin D Receptor

Nutrients ◽

10.3390/nu13051715 ◽

2021 ◽

Vol 13 (5) ◽

pp. 1715

Author(s):

Ye Li ◽

Katherine L Cook ◽

Wei Yu ◽

Lu Jin ◽

Kerrie B Bouker ◽

...

Keyword(s):

Breast Cancer ◽

Vitamin D ◽

Cancer Patients ◽

Vitamin D Receptor ◽

Tamoxifen Resistance ◽

Cancer Recurrence ◽

Synthetic Analog ◽

Breast Cancer Patients ◽

Jnk Pathway ◽

Estrogen Receptor Positive

We determined how vitamin D receptor (VDR) is linked to disease outcome in estrogen receptor-positive (ER+) breast cancer patients treated with tamoxifen (TAM). Breast cancer patients (n = 581) in four different datasets were divided into those expressing higher (above median) and lower levels of VDR in pretreatment ER+ tumors. Across all datasets, TAM-treated patients with higher pretreatment tumor VDR expression exhibited significantly longer recurrence-free survival. Ingenuity pathway analysis identified autophagy and unfolded protein response (UPR) as top differentially expressed pathways between high and low VDR-expressing ER+ cancers. Activation of VDR with vitamin D (VitD), either calcitriol or its synthetic analog EB1089, sensitized MCF-7-derived, antiestrogen-resistant LCC9 human breast cancer cells to TAM, and attenuated increased UPR and pro-survival autophagy. Silencing of VDR blocked these effects through the IRE1α-JNK pathway. Further, silencing of VDR impaired sensitivity to TAM in antiestrogen-responsive LCC1 cells, and prevented the effects of calcitriol and EB1089 on UPR and autophagy. In a preclinical mouse model, dietary VitD supplementation induced VDR activation and reduced carcinogen-induced ER+ mammary tumor incidence. In addition, IRE1α-JNK signaling was downregulated and survival autophagy was inhibited in mammary tumors of VitD-supplemented mice. Thus, activation of VDR is predictive of reduced risk of breast cancer recurrence in ER+ patients, possibly by inhibiting antiestrogen-promoted pro-survival autophagy.

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