Nothapodytes nimmoniana: A Review on Phytochemical and Pharmacological Aspects

2021 ◽  
pp. 313-322
Author(s):  
V. Sornalakshmi ◽  
P. S. Tresina ◽  
K. Paulpriya ◽  
V. R. Mohan
Keyword(s):  
Nothapodytes Nimmoniana

scholarly journals Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
I. A. H. Khwajah Mohinudeen ◽  
Saumya Pandey ◽  
Hemalatha Kanniyappan ◽  
Vignesh Muthuvijayan ◽  
Smita Srivastava
Keyword(s):  
Natural Habitat ◽  
Product Yield ◽  
Study Strategies ◽  
Selective Agent ◽  
Nothapodytes Nimmoniana ◽  
Time Consumption ◽  
Potential Alternative ◽  
Plant Metabolite ◽  
Produce Plant ◽  
Selection Of

AbstractEndophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.

scholarly journals Seasonal variation in content of camptothecin from the bark of Nothapodytes nimmoniana (Grah.) Mabb., using HPLC analysis

2013 ◽  
Vol 5 (3) ◽  
pp. 219 ◽  
Author(s):  
SandeepR Pai ◽  
NileshV Pawar ◽  
MansingrajS Nimbalkar ◽  
ParthrajR Kshirsagar ◽  
FirdoseK Kolar ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Hplc Analysis ◽  
Nothapodytes Nimmoniana

scholarly journals HYDROALCOHOLIC EXTRACTION OF SHOOT CULTURES FROM NOTHAPODYTE NIMMONIANA AND ITS ANTI-PROLIFERATIVE ANALYSIS

2018 ◽  
Vol 10 (6) ◽  
pp. 32
Author(s):  
Arun Sharma ◽  
Ankita Rajata ◽  
Udayabanu Malairaman ◽  
Hemant Sood
Keyword(s):  
Hplc Analysis ◽  
Soxhlet Extraction ◽  
Multiple Shoots ◽  
Alcoholic Extract ◽  
Shoot Cultures ◽  
Old Field ◽  
Nothapodytes Nimmoniana ◽  
In Vitro Shoots ◽  
Ethidium Bromide Staining

Objective: The important restraints in plant-derived medicine are the convenience of active composites which depends on diverse topographical situations, active compound build-up pattern, environmental circumstances and genetic makeup of the plant. The presented work was aimed to describe an efficient technique for fast propagation of Nothapodytes nimmoniana which is an imperative source of Camptothecin.Methods: Multiple shoots were successfully regenerated from the explants N. nimmoniana using Murashige and Skoog (MS) medium accompanied with IBA+KN+GA3 (1:3:2) mg/l. the hydro-alcoholic extract was obtained by soxhlet extraction for field grown roots (fgRE), shoots (fgSE) and in vitro plantlets (ivPL) and further subjected to phytochemicals analysis. HPLC analysis was performed to determine the percentage of camptothecin (CAM). Anti-proliferative studies were carried out followed by Acridine orange/Ethidium bromide staining. Extracts were also estimated for reactive nitrogen species (RNS) and reactive oxygen species (ROS).Results: Preliminary qualitative chemical analysis of the extracts displayed the presence of alkaloids, carbohydrates, steroids, saponins, terpenoids and phenolics compounds. HPLC analysis revealed the presence of CAM in all the extracts. ivPL exhibited lowest anti-proliferative activity in contrast to other extract and percentage of ROS and RNS was also less. Although ivPL did not exhibited prominent anti-tumour effect, but in vitro accumulation of CAM in 2-months old shoots provides an alternative resource to 5 y-old field grown plants.Conclusion: This work delivers a potential to lead to further increase the potency of CAM in in vitro shoots of two months old as an alternative to the field grown plants for treating cancers.

Molecular characterization and overexpression analyses of secologanin synthase to understand the regulation of camptothecin biosynthesis in Nothapodytes nimmoniana (Graham.) Mabb.

PROTOPLASMA ◽  
2019 ◽  
Vol 257 (2) ◽  
pp. 391-405 ◽  
Author(s):  
Gulzar A. Rather ◽  
Arti Sharma ◽  
Prashant Misra ◽  
Amit Kumar ◽  
Veenu Kaul ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Nothapodytes Nimmoniana

Phytochemical and pharmacological aspects of Nothapodytes nimmoniana. An overview

2013 ◽  
Vol 59 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Nazeerullah Khan ◽  
Ennus Tajuddin Tamboli ◽  
V.K. Sharma ◽  
Sunil Kumar
Keyword(s):  
Wide Spectrum ◽  
Pharmacological Activities ◽  
Nothapodytes Nimmoniana ◽  
Jammu And Kashmir ◽  
Medicinal Uses ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Fungal ◽  
Anti Hiv ◽  
Important Medicinal Plant

Summary Nothapodytes nimmoniana (J. Graham) (Icacinaceae), commonly known as Amruta is found in India particularly in Maharashtra, Goa, Kerala, Assam, Jammu and Kashmir as well as Tamilnadu areas. It is an important medicinal plant, the major source of a potent alkaloid, namely camptothecin, of a wide spectrum of pharmacological activities like anti-cancer, anti-HIV, antimalarial, antibacterial, anti-oxidant, anti-inflammatory, anti-fungal and also applied in the treatment of anaemia. Camptothecin is still not synthesized, therefore, its production entirely depends on natural sources. N. nimmoniana is one such plant which yields contain camptothecin in significantly high amount. The plant is gaining international recognition due to its diversified medicinal uses. It is subjected to excessive harvest. It has been categorized as a vulnerable and endangered plant. The present review encompasses the phytochemical, analytical, pharmacological, biotechnological, and other specific aspects of N. nimmoniana.

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