scholarly journals Screening and selection of camptothecin producing endophytes from Nothapodytes nimmoniana

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
I. A. H. Khwajah Mohinudeen ◽  
Saumya Pandey ◽  
Hemalatha Kanniyappan ◽  
Vignesh Muthuvijayan ◽  
Smita Srivastava
Keyword(s):  
Natural Habitat ◽  
Product Yield ◽  
Study Strategies ◽  
Selective Agent ◽  
Nothapodytes Nimmoniana ◽  
Time Consumption ◽  
Potential Alternative ◽  
Plant Metabolite ◽  
Produce Plant ◽  
Selection Of

AbstractEndophytic fungi with the ability to produce plant based secondary metabolites are a potential alternative for producing the host plant metabolite and to prevent natural plants from extinction. To isolate a high metabolite yielding endophytic strain from plants, hundreds of endophytic strains are screened and tested for product yield separately under axenic state, before shortlisting the potential endophyte, which involves huge time consumption. In this study, strategies for screening and selection of high camptothecin yielding endophytes from their natural habitat were proposed. A correlation was built between the camptothecin yield in the explants and the endophytes isolated from them. In addition, camptothecin yield was compared between the endophytes isolated from young and matured plants. Further, camptothecin producers and non-producers strains were compared for their tolerance toward camptothecin. The study indicates that high camptothecin yielding endophytes were isolated from high yielding explants and younger plants and they were more tolerant to camptothecin in comparison to non-camptothecin yielding endophytes. Thus, choosing a young and high yielding explant for endophyte isolation, and use of camptothecin as a selective agent in the growth medium, can be instrumental in screening and selection of high camptothecin yielding endophytes from nature in relatively less time.

scholarly journals Selection of Best Test Cases using Support Vector Machine for ARPT

2019 ◽  
Vol 8 (3) ◽  
pp. 4265-4271
Keyword(s):  
Support Vector Machine ◽  
Software Testing ◽  
Support Vector ◽  
Test Cases ◽  
Testing Strategy ◽  
Random Partition ◽  
Code Coverage ◽  
Time Consumption ◽  
Branch Coverage ◽  
Selection Of

Software testing is an essential activity in software industries for quality assurance; subsequently, it can be effectively removing defects before software deployment. Mostly good software testing strategy is to accomplish the fundamental testing objective while solving the trade-offs between effectiveness and efficiency testing issues. Adaptive and Random Partition software Testing (ARPT) approach was a combination of Adaptive Testing (AT) and Random Partition Approach (RPT) used to test software effectively. It has two variants they are ARPT-1 and ARPT-2. In ARPT-1, AT was used to select a certain number of test cases and then RPT was used to select a number of test cases before returning to AT. In ARPT-2, AT was used to select the first m test cases and then switch to RPT for the remaining tests. The computational complexity for random partitioning in ARPT was solved by cluster the test cases using a different clustering algorithm. The parameters of ARPT-1 and ARPT-2 needs to be estimated for different software, it leads to high computation overhead and time consumption. It was solved by Improvised BAT optimization algorithms and this approach is named as Optimized ARPT1 (OARPT1) and OARPT2. By using all test cases in OARPT will leads to high time consumption and computational overhead. In order to avoid this problem, OARPT1 with Support Vector Machine (OARPT1-SVM) and OARPT2- SVM are introduced in this paper. The SVM is used for selection of best test cases for OARPT-1 and OARPT-2 testing strategy. The SVM constructs hyper plane in a multi-dimensional space which is used to separate test cases which have high code and branch coverage and test cases which have low code and branch coverage. Thus, the SVM selects the best test cases for OARPT-1 and OARPT-2. The selected test cases are used in OARPT-1 and OARPT-2 to test software. In the experiment, three different software is used to prove the effectiveness of proposed OARPT1- SVM and OARPT2-SVM testing strategies in terms of time consumption, defect detection efficiency, branch coverage and code coverage.

Bacteriophages: from isolation to application

2021 ◽  
Vol 22 ◽  
Author(s):  
Abdallah S. Abdelsattar ◽  
Alyaa Dawoud ◽  
Salsabil Makky ◽  
Rana Nofal ◽  
Ramy K. Aziz ◽  
...  
Keyword(s):  
Food Industry ◽  
Pathogenic Bacteria ◽  
Phage Genome ◽  
Morphological Structure ◽  
High Specificity ◽  
Potential Alternative ◽  
And Behavior ◽  
Phage Isolation ◽  
Different Levels ◽  
Selection Of

: Bacteriophages are considered as a potential alternative to fight pathogenic bacteria during the antibiotic resistance era. With their high specificity, they are being widely used in various applications: medicine, food industry, agriculture, animal farms, biotechnology, diagnosis, etc. Many techniques have been designed by different researchers for phage isolation, purification, and amplification, each of which has strengths and weaknesses. However, all aim at having a reasonably pure phage sample that can be further characterized. Phages can be characterized based on their physiological, morphological or inactivation tests. Microscopy, in particular, has opened a wide gate not only for visualizing phage morphological structure, but also for monitoring biochemistry and behavior. Meanwhile, computational analysis of phage genomes provides more details about phage history, lifestyle, and potential for toxigenic or lysogenic conversion, which translate to safety in biocontrol and phage therapy applications. This review summarizes phage application pipelines at different levels and addresses specific restrictions and knowledge gaps in the field. Recently developed computational approaches, which are used in phage genome analysis, are critically assessed. We hope that this assessment provides researchers with useful insights for selection of suitable approaches for Phage-related research aims and applications.

scholarly journals Batch pyrolysis of cotton stalks for evaluation of biochar energy potential

2019 ◽  
Vol 116 ◽  
pp. 00001 ◽  
Author(s):  
Rafat Al Afif ◽  
S. Sean Anayah ◽  
Christoph Pfeifer
Keyword(s):  
Pyrolysis Temperature ◽  
Product Yield ◽  
Energy Yield ◽  
Mass Energy ◽  
Energy Potential ◽  
Pyrolysis Products ◽  
Heating Value ◽  
Cotton Stalks ◽  
Bio Oil ◽  
Selection Of

The thermal cracking of cotton stalks (CS) via pyrolysis was performed using a laboratory scale batch pyrolysis reactor. The effects of the final pyrolysis temperature varying from 300 to 800°C on the pyrolysis products distribution has been investigated. The maximum biochar yield of 46.5% was obtained at 400°C. As the pyrolysis process temperature increased, the solid char product yield decreased. The lowest biochar yield of 28% was obtained at 800°C. The largest higher heating value (HHV, 25.845 MJ kg-1) was obtained at 600°C. All biochar samples produced between 500 and 700°C had an energy densification ratio of 1.41, indicating a higher mass-energy density than the initial feedstock. A larger share of syngas and bio-oil were produced at higher temperatures, as estimated. Preferential selection of a char based on the energy yield would lead to a selection of the 400°C product, while selection based on the energy densification ratio would be for a product obtained between 500 to 700°C.

scholarly journals Pomegranate as a Potential Alternative of Pain Management: A Review

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 419
Author(s):  
José Antonio Guerrero-Solano ◽  
Osmar Antonio Jaramillo-Morales ◽  
Claudia Velázquez-González ◽  
Minarda De la O-Arciniega ◽  
Araceli Castañeda-Ovando ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Scientific Evidence ◽  
Antinociceptive Effect ◽  
Punica Granatum ◽  
Mechanisms Of Action ◽  
Inclusion Criteria ◽  
Potential Alternative ◽  
Punica Granatum L ◽  
Different Parts ◽  
Selection Of

The use of complementary medicine has recently increased in an attempt to find effective alternative therapies that reduce the adverse effects of drugs. Punica granatum L. (pomegranate) has been used in traditional medicine for different kinds of pain. This review aims to explore the scientific evidence about the antinociceptive effect of pomegranate. A selection of original scientific articles that accomplished the inclusion criteria was carried out. It was found that different parts of pomegranate showed an antinociceptive effect; this effect can be due mainly by the presence of polyphenols, flavonoids, or fatty acids. It is suggested in the literature that the mechanisms of action may be related to the activation of the L-arginine / NO pathway, members of the TRP superfamily (TRPA1 or TRPV1) and the opioid system. The implications for the field are to know the mechanisms of action by which this effect is generated and thus be able to create alternative treatments for specific types of pain, which help alleviate it and reduce the adverse effects produced by drugs. The results propose that pomegranate and secondary metabolites could be considered in the treatment of inflammatory, nociceptive, and neuropathic pain.

scholarly journals Regio- and Stereoselective Fungal Oxyfunctionalisation of Limonenes

2005 ◽  
Vol 60 (5-6) ◽  
pp. 459-466 ◽  
Author(s):  
Rüdiger Kaspera ◽  
Ulrich Krings ◽  
Michael Pescheck ◽  
Dieter Sell ◽  
Jens Schrader ◽  
...  
Keyword(s):  
Optical Purity ◽  
Product Yield ◽  
Careful Selection ◽  
Fed Batch ◽  
Batch Mode ◽  
Theoretical Yield ◽  
Pleurotus Sapidus ◽  
Terminal Oxidation ◽  
By Products ◽  
Selection Of

Selective transformations of limonene by asco- and basidiomycetes were investigated. On the shake flask scale, Penicillium citrinum hydrated R-(+)-limonene to α-terpineol [83% regioselectivity (rs), more than 80 mg 1-1 product yield], and Gongronella butleri catalysed the terminal oxidation to yield perillyl alcohol (60% rs, 16 mg 1-1). On the laboratory bioreactor scale, Penicillium digitatum produced a peak concentration of 506 mg α-terpineol 1-1 in the fed-batch mode, equivalent to a theoretical yield of 67%, and no volatile by-products were found. Fusarium proliferatum transformed R-(+)-limonene enantiospecifically to cis-(+)- carveol (98.6% ee, more than 35 mg 1-1 product yield) and S-(-)-limonene predominantly to trans-(-)-carveol (96.3% ee). Pleurotus sapidus selectively dehydrogenised the accumulating trans-(-)-carveol to the corresponding enantiopure R-(-)-carvone. The results show that a careful selection of strain and bioprocess parameters may improve both the yield and the optical purity of a desired product.

scholarly journals Learning and Study Strategies: Academic Achievement and Gender Differences

2013 ◽  
Vol 12 (4) ◽  
pp. 49
Author(s):  
Beena Daliya R ◽  
Sudha Bhogle
Keyword(s):  
Academic Achievement ◽  
Gender Differences ◽  
High School Students ◽  
Academic Success ◽  
Research Work ◽  
Study Strategies ◽  
School Students ◽  
Main Ideas ◽  
Learning And Study Strategies ◽  
Selection Of

To do well in school and for enhanced academic success, effective Learning and Study strategies are important. The objectives of this research work were to determine gender differences, if any, in Learning and Study strategies in high school students and to find the relationship between these strategies and Academic achievement. To do this, Learning and Study Strategies Inventory (LASSI) - Weinstein & Palmer, (2002) was administered to a group of 684, 8th std., school students, of which 363 were boys and 321 were girls. These students were selected from private schools of Bangalore; all the 4 zones – north, south, east, and west - were represented. All the students chosen were studying the state syllabus. The results of the research indicate gender differences in the following strategies – processing of information, motivation and selection of main ideas. No gender differences emerged for Academic achievement. Further, academic achievement was found to be significantly related to levels of anxiety, attitude, concentration ability, selection of main ideas, effective time management and test taking strategies.Keywords: Learning strategies, Study strategies, Academic Achievement, Gender difference.

scholarly journals SELECTION OF FUNGAL ISOLATES WITH POTENTIAL FOR PHOSPHATE SOLUBILIZATION AND FORMULATION OF INOCULANT FOR COFFEE CROPS

2019 ◽  
Vol 14 (3) ◽  
pp. 315
Author(s):  
Sara Maria Chalfoun ◽  
Caroline Lima Angélico ◽  
Mário Lúcio Vilela De Resende ◽  
Graziella Evaristo De Moraes
Keyword(s):  
Water Contamination ◽  
Phosphate Solubilization ◽  
Low Cost ◽  
Soil Health ◽  
Phosphorus Sources ◽  
Potential Alternative ◽  
Synthetic Fertilizers ◽  
Low Solubility ◽  
Global Food ◽  
Selection Of

The dependence on chemical fertilizers for the future of agriculture will result in additional soil health losses, possibility of water contamination and continued currency avoidance due to the increasing dependence on imports. The indiscriminate use of synthetic fertilizers has polluted the soil and watersheds, destroying useful microorganisms and insects, what makes crops more susceptible to diseases. Considering this critical scenario, microorganisms have stood out as a potential alternative for maintaining the productivity, reliability and sustainability of the global food chain. In order to increase the productivity and/or reduce the amount of fertilizers, as well as to meet the requirements for the use of low-solubility phosphorus sources, microorganisms were isolated from the rhizosphere and artificially cultivated. Subsequently, they were selected regarding their potential for solubilizing phosphorus and incorporated into a low-cost matrix. A microbial inoculant was thus developed and tested in a coffee crop. It was possible to formulate an inoculant based on an isolate selected from <em>Aspergillus niger</em>, using an inactivated seed and sodium alginate as matrices. It was also observed that there was a greater correlation between the evaluated parameters and increasing doses of the inoculants when Araxá apatite was used as source of phosphorus, proving the importance of the inoculants as release factors and availability of phosphorus in the case of sources less soluble, giving Brazilian agriculture greater autonomy in the input market.

scholarly journals Abiraterone In Vitro Is Superior to Enzalutamide in Response to Ionizing Radiation

2021 ◽  
Vol 11 ◽  
Author(s):  
Timothy C. Wright ◽  
Victoria L. Dunne ◽  
Ali H. D. Alshehri ◽  
Kelly M. Redmond ◽  
Aidan J. Cole ◽  
...  
Keyword(s):  
Synergistic Effects ◽  
Progression Free Survival ◽  
Abiraterone Acetate ◽  
Cell Cycle Distribution ◽  
Castration Resistant Prostate Cancer ◽  
Castration Resistant ◽  
Potential Alternative ◽  
Underlying Mechanisms ◽  
Selection Of

Abiraterone acetate and Enzalutamide are novel anti-androgens that are key treatments to improve both progression-free survival and overall survival in patients with metastatic castration-resistant prostate cancer. In this study, we aimed to determine whether combinations of AR inhibitors with radiation are additive or synergistic, and investigated the underlying mechanisms governing this. This study also aimed to compare and investigate a biological rationale for the selection of Abiraterone versus Enzalutamide in combination with radiotherapy as currently selection is based on consideration of side effect profiles and clinical experience. We report that AR suppression with Enzalutamide produces a synergistic effect only in AR-sensitive prostate models. In contrast, Abiraterone displays synergistic effects in combination with radiation regardless of AR status, alluding to potential alternative mechanisms of action. The underlying mechanisms governing this AR-based synergy are based on the reduction of key AR linked DNA repair pathways such as NHEJ and HR, with changes in HR potentially the result of changes in cell cycle distribution, with these reductions ultimately resulting in increased cell death. These changes were also shown to be conserved in combination with radiation, with AR suppression 24 hours before radiation leading to the most significant differences. Comparison between Abiraterone and Enzalutamide highlighted Abiraterone from a mechanistic standpoint as being superior to Abiraterone for all endpoints measured. Therefore, this provides a potential rationale for the selection of Abiraterone over Enzalutamide.

Green Solvent: Green Shadow on Chemical Synthesis

2020 ◽  
Vol 17 (6) ◽  
pp. 426-439
Author(s):  
Tejaswini Sahoo ◽  
Jagannath Panda ◽  
Jnanaranjan Sahu ◽  
Dayananda Sarangi ◽  
Sunil K. Sahoo ◽  
...  
Keyword(s):  
Green Chemistry ◽  
Chemical Synthesis ◽  
Biological Effects ◽  
Natural Habitat ◽  
Waste Product ◽  
Chemical Properties ◽  
Reaction Medium ◽  
Green Solvents ◽  
Increased Risk ◽  
Selection Of

The natural beauty and purity of our planet has been contaminated deeply due to human selfish activities such as pollution, improper waste management, and various industrial and commercial discharges of untreated toxic by-products into the lap of nature. The collective impact of these hazardous suspensions into the natural habitat is very deadly. Challenges due to human activity on the environment have become ubiquitous. The chemical industry has a major role in human evolution and, predictably, opened gates of increased risk of pollution if the production is not done sustainably. In these circumstances, the notion of Green Chemistry has been identified as the efficient medium of synthesis of chemicals and procedures to eradicate the toxic production of harmful substances. Principles of Green Chemistry guide the scientist in their hunt towards chemical synthesis which requires the use of solvents. These solvents contaminate our air, water, land and surrounding due to its toxic properties. Even though sufficient precautions are taken for proper disposal of these solvents but it is difficult to be recycled. In order to preserve our future and coming generation from the adverse impacts associated with solvents it is very important to find alternative of this which will be easy to use, reusable and also eco-friendly. Solvents are used daily in various industrial processes as reaction medium, as diluters, and in separation procedures. As reaction medium, the role of solvent is to bring catalysts and reactants together and to release heat thus affecting activity and selectivity. The proper selection of the solvent considering its biological, physical and chemical properties is very necessary for product separation, environmental, safety handling and economic factors. Green solvents are the boon in this context. They are not only environmentally benign but also cost effective. The biggest challenge faced by the chemists is adaptation of methods and selection of solvents during chemical synthesis which will give negligible waste product and will remain human and nature friendly. During designing compounds for a particular reaction it is difficult to give assurance regarding the toxicity and biodegradability of the method. Chemists are still far away from predicting the various chemical and biological effects of the compounds on the back of the envelope. To achieve that point is formidable task but it will definitely act as inspiration for the coming generation of chemists. The green solvents are undoubtedly a far better approach to eliminate the negative impacts and aftermath of any chemical synthesis on the environment. Our study in this review covers an overview of green solvents, their role in safer chemical synthesis with reference to some of the important green solvents and their detail summarization.

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