Genetically Engineered Resistance: Transgenic Plants

Plant Viruses ◽  
2018 ◽  
pp. 347-363 ◽  
Author(s):  
C. Hemenway ◽  
L. Haley ◽  
W. K. Kaniewski ◽  
E. C. Lawson ◽  
K. M. O'Connell ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Genetically Engineered ◽  
Engineered Resistance

Morphological And Immunocytochemical Analysis Of Polyhydroxybuterate Distribution In Transgenic Plants

1999 ◽  
Vol 5 (S2) ◽  
pp. 1254-1255
Author(s):  
S.M. Colburn ◽  
N. Biest ◽  
M. Hao ◽  
K. Houmiel ◽  
T. Mitsky ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Credit Cards ◽  
Genetically Engineered ◽  
Crop Plants ◽  
Attractive Alternative ◽  
Immunocytochemical Analysis ◽  
Trade Name ◽  
Phb Production ◽  
Plastic Products

Poly-β-hydroxybuterate (PHB), a biodegradable polyester, is accumulated as a storage compound in many species of bacteria. It belongs to a class of polymers called polyhydroxyalkanoates (PHAs). PHA's were originally identified in 1923.However these polymers first came to the attention of industry in the early 1980's when they were recognized as having thermoplastic properties.Monsanto currently markets a form of PHA produced via fermentation under the trade name Biopol™. It is used to make biodegradable plastic products such as credit cards, bottles and disposable drinking cups. However fermentation is an expensive route to production. The use of crop plants genetically engineered to produce PHA's could provide a less expensive source of the plastic and therefore a more attractive alternative to traditional non-biodegradable petrochemical derived plastics. PHB production in plants was first demonstrated in 1992 in transgenic Arabidopsis thaliana by Chris Somerville and coworkers. They showed that PHB production was tolerated by the plant if it was targeted to the chloroplasts.

Genetically Engineered Resistance to Dengue-2 Virus Transmission in Mosquitoes

Science ◽  
1996 ◽  
Vol 272 (5263) ◽  
pp. 884-886 ◽  
Author(s):  
K. E. Olson ◽  
S. Higgs ◽  
P. J. Gaines ◽  
A. M. Powers ◽  
B. S. Davis ◽  
...  
Keyword(s):  
Virus Transmission ◽  
Genetically Engineered ◽  
Engineered Resistance

Genetically engineered resistance against grapevine chrome mosaic nepovirus

1993 ◽  
Vol 21 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Véronique Brault ◽  
Thierry Candresse ◽  
Olivier le Gall ◽  
René Pierre Delbos ◽  
Maryvonne Lanneau ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Engineered Resistance

scholarly journals RNA interference-mediated tolerance to whitefly (Bemisia tabaci) in genetically engineered tomato

2021 ◽  
Author(s):  
Carolina Senhorinho Ramalho Pizetta ◽  
William Rafael Ribeiro ◽  
Amanda Lopes Ferreira ◽  
Matheus da Costa Moura ◽  
Kenny Bonfim ◽  
...  
Keyword(s):  
Rna Interference ◽  
Transgenic Plants ◽  
Bemisia Tabaci ◽  
Insect Pest ◽  
Genetically Engineered ◽  
Horticultural Crops ◽  
Transgenic Tomato Plants ◽  
Atpase Gene ◽  
Transgenic Lines ◽  
Polyphagous Insect

Abstract Whitefly (Bemisia tabaci) is a polyphagous insect that causes huge damage in several horticultural crops, including tomato, by sucking nutrients from the phloem and transmitting viruses. Whiteflies are particularly difficult to manage and the use of chemicals remains the common practice, which causes the development of insecticidal resistance. Thus, there is considerable interest in the introduction of whitefly resistance by classical and molecular breeding. Here, we explored the concept of using an RNA interference construct to silence a v-ATPase gene in whiteflies interacting with transgenic tomato plants that express siRNA molecules corresponding to a fragment from the B. tabaci vATPase. PCR analyses revealed the presence of both ΔATPase and nptII transgenes in all transgenic lines. siRNA expressing lines were challenged against whitefly and revealed a mortality rate of 57.1% in transgenic line 4.4.1, while in the control the mortality was 7.6%. Mortality of 2nd instar nymphs was higher on the transgenic plants and the development of 3rd instar nymphs was slightly longer than on the control plants. Although the attraction of insects was not significantly different between treatments, the number of eggs laid by the insects on the transgenic plants was significantly lower, compared to the controls. RT-qPCR revealed a decreased expression level of endogenous v-ATPase gene in whiteflies feeding on transgenic plants. No unexpected effect was observed on the non-target insects Myzus persicae or Tuta absoluta. Results presented here may form the foundation for the generation of elite tomato varieties resistant to whitefly, a devastating insect pest.

scholarly journals The production of very-long-chain PUFA biosynthesis in transgenic plants: towards a sustainable source of fish oils

2005 ◽  
Vol 64 (3) ◽  
pp. 387-393 ◽  
Author(s):  
Johnathan A. Napier ◽  
Olga Sayanova
Keyword(s):  
Fatty Acids ◽  
Transgenic Plants ◽  
Genetically Engineered ◽  
Fish Oils ◽  
Alternative Source ◽  
Fish Stocks ◽  
Chain Pufa ◽  
Pufa Biosynthesis ◽  
Over Exploitation ◽  
Long Chain

There is now considerable evidence of the importance of n-3 long-chain PUFA in human health and development. At the same time, the marine fish stocks that serve as the primary sources of these fatty acids are threatened by continued over-exploitation. Thus, there is an urgent need to provide a sustainable alternative source of the n-3 long-chain PUFA normally found in fish oils. The possibility of using transgenic plants genetically engineered to synthesise these important fatty acids has recently been demonstrated. The approaches taken to realise this outcome will be discussed, as will their prospects for providing a sustainable resource for the future.

Bacterial nitrous oxide reductase expressed in transgenic plants: Evidence for sufficient anaerobicity to permit activity

2012 ◽  
Vol 92 (7) ◽  
pp. 1283-1294
Author(s):  
Shen Wan ◽  
Kagami Goto ◽  
Yaseen Mottiar ◽  
Julianne M. Staebler ◽  
Amanda M. Johnson ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Transgenic Plants ◽  
Crop Yields ◽  
Pseudomonas Stutzeri ◽  
Genetically Engineered ◽  
Nitrous Oxide Reductase ◽  
In Planta ◽  
Accessory Gene ◽  
Genetically Engineered Crops ◽  
Anaerobic Environment

Wan, S., Goto, K., Mottiar, Y., Staebler, J. M., Johnson, A. M., Voronova, A., Blais, D. R., Zaidi, M. A. and Altosaar, I. 2012. Bacterial nitrous oxide reductase expressed in transgenic plants: Evidence for sufficient anaerobicity to permit activity. Can. J. Plant Sci. 92: 1283–1294. Soil nitrogen enrichment practices enhance crop yields but greatly exacerbate the production and release of nitrous oxide (N2O), a potent greenhouse gas. The conversion of N2O to dinitrogen (N2) in soil denitrifiers is normally driven by the anaerobic enzyme nitrous oxide reductase (N2OR). Since this step is often limited in fertilised soils, we have transferred this unique microbial biocatalyst from the soil bacterium Pseudomonas stutzeri to transgenic Nicotiana tabacum plants. Our results confirm that engineered plants expressing the N2OR structural gene nosZ are capable of producing functional reductase enzyme without the involvement of the native accessory gene products. Since bacterial N2OR normally exists in the anaerobic environment of the soil bacterium's periplasmic space, this novel in planta activity suggests that plant cells can provide a similar hypoxic refuge for anaerobic enzyme activity. Genetically engineered crops containing N2OR could have considerable potential in the phytoremediation of atmospheric N2O.

Are the PR1 proteins of tobacco involved in genetically engineered resistance to TMV?

Virology ◽  
1989 ◽  
Vol 169 (2) ◽  
pp. 470-473 ◽  
Author(s):  
John P. Carr ◽  
Roger N. Beachy ◽  
Daniel F. Klessig
Keyword(s):  
Genetically Engineered ◽  
Engineered Resistance
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