Morphological And Immunocytochemical Analysis Of Polyhydroxybuterate Distribution In Transgenic Plants

1999 ◽  
Vol 5 (S2) ◽  
pp. 1254-1255
Author(s):  
S.M. Colburn ◽  
N. Biest ◽  
M. Hao ◽  
K. Houmiel ◽  
T. Mitsky ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Credit Cards ◽  
Genetically Engineered ◽  
Crop Plants ◽  
Attractive Alternative ◽  
Immunocytochemical Analysis ◽  
Trade Name ◽  
Phb Production ◽  
Plastic Products

Poly-β-hydroxybuterate (PHB), a biodegradable polyester, is accumulated as a storage compound in many species of bacteria. It belongs to a class of polymers called polyhydroxyalkanoates (PHAs). PHA's were originally identified in 1923.However these polymers first came to the attention of industry in the early 1980's when they were recognized as having thermoplastic properties.Monsanto currently markets a form of PHA produced via fermentation under the trade name Biopol™. It is used to make biodegradable plastic products such as credit cards, bottles and disposable drinking cups. However fermentation is an expensive route to production. The use of crop plants genetically engineered to produce PHA's could provide a less expensive source of the plastic and therefore a more attractive alternative to traditional non-biodegradable petrochemical derived plastics. PHB production in plants was first demonstrated in 1992 in transgenic Arabidopsis thaliana by Chris Somerville and coworkers. They showed that PHB production was tolerated by the plant if it was targeted to the chloroplasts.

scholarly journals Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus

1999 ◽  
Vol 22 (2) ◽  
pp. 159-167 ◽  
Author(s):  
E. S. JENKINS ◽  
W. PAUL ◽  
M. CRAZE ◽  
C. A. WHITELAW ◽  
A. WEIGAND ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Transgenic Plants ◽  
Gene Encoding

scholarly journals Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

2004 ◽  
Vol 109 (7) ◽  
pp. 1512-1518 ◽  
Author(s):  
M. Labra ◽  
C. Vannini ◽  
F. Grassi ◽  
M. Bracale ◽  
M. Balsemin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Genomic Stability ◽  
Floral Dip

Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

2020 ◽  
Vol 47 (5) ◽  
pp. 454
Author(s):  
Jian Li ◽  
Tian Chen ◽  
Fengzhen Huang ◽  
Penghui Dai ◽  
Fuxiang Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Seed Abortion ◽  
Transgenic Seeds ◽  
Genes Encoding ◽  
Colour Changes ◽  
R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

scholarly journals Industrial Production of Poly-β-hydroxybutyrate from CO2: Can Cyanobacteria Meet this Challenge?

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 323 ◽  
Author(s):  
Roberta Carpine ◽  
Giuseppe Olivieri ◽  
Klaas J. Hellingwerf ◽  
Antonino Pollio ◽  
Antonio Marzocchella
Keyword(s):  
Low Cost ◽  
Operation Mode ◽  
Commercial Production ◽  
Continuous Operation ◽  
Attractive Alternative ◽  
Bacterial Fermentation ◽  
Plastic Materials ◽  
Phb Production ◽  
Global Concern ◽  
Environmentally Friendly Process

The increasing impact of plastic materials on the environment is a growing global concern. In regards to this circumstance, it is a major challenge to find new sources for the production of bioplastics. Poly-β-hydroxybutyrate (PHB) is characterized by interesting features that draw attention for research and commercial ventures. Indeed, PHB is eco-friendly, biodegradable, and biocompatible. Bacterial fermentation processes are a known route to produce PHB. However, the production of PHB through the chemoheterotrophic bacterial system is very expensive due to the high costs of the carbon source for the growth of the organism. On the contrary, the production of PHB through the photoautotrophic cyanobacterium system is considered an attractive alternative for a low-cost PHB production because of the inexpensive feedstock (CO2 and light). This paper regards the evaluation of four independent strategies to improve the PHB production by cyanobacteria: (i) the design of the medium; (ii) the genetic engineering to improve the PHB accumulation; (iii) the development of robust models as a tool to identify the bottleneck(s) of the PHB production to maximize the production; and (iv) the continuous operation mode in a photobioreactor for PHB production. The synergic effect of these strategies could address the design of the optimal PHB production process by cyanobacteria. A further limitation for the commercial production of PHB via the biotechnological route are the high costs related to the recovery of PHB granules. Therefore, a further challenge is to select a low-cost and environmentally friendly process to recover PHB from cyanobacteria.

scholarly journals Molecular Cloning and Functional Characterization of CpMYC2 and CpBHLH13 Transcription Factors from Wintersweet (Chimonanthus praecox L.)

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 785
Author(s):  
Muhammad Zeshan Aslam ◽  
Xiang Lin ◽  
Xiang Li ◽  
Nan Yang ◽  
Longqing Chen
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Transgenic Plants ◽  
Jasmonic Acid ◽  
Volatile Compounds ◽  
Floral Scent ◽  
Functional Characterization ◽  
High Expression ◽  
Floral Volatile ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox L.) is an ornamental and economically significant shrub known for its unique flowering characteristics, especially the emission of abundant floral volatile organic compounds. Thus, an understanding of the molecular mechanism of the production of these compounds is necessary to create new breeds with high volatile production. In this study, two bHLH transcription factors (CpMYC2 and CpbHLH13) of Wintersweet H29 were functionally characterized to illustrate their possible role in the production of volatile compounds. The qRT-PCR results showed that the expression of CpMYC2 and CpbHLH13 increased from the flower budding to full bloom stage, indicating that these two genes may play an essential role in blooming and aroma production in wintersweet. Gas chromatography-mass spectroscopy (GC-MS) analysis revealed that the overexpression of CpMYC2 in arabidopsis (Arabidopsis thaliana) AtMYC2-2 mutant (Salk_083483) and tobacco (Nicotiana tabaccum) genotype Petit Havana SR1 significantly increased floral volatile monoterpene, especially linalool, while the overexpression of CpbHLH13 in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and tobacco genotype SR1 increased floral sesquiterpene β-caryophyllene production in both types of transgenic plants respectively. High expression of terpene synthase (TPS) genes in transgenic A. thaliana along with high expression of CpMYC2 and CpbHLH13 in transgenic plants was also observed. The application of a combination of methyl jasmonic acid (MeJA) and gibberellic acid (GA3) showed an increment in linalool production in CpMYC2-overexpressing arabidopsis plants, and the high transcript level of TPS genes also suggested the involvement of CpMYC2 in the jasmonic acid (JA) signaling pathway. These results indicate that both the CpMYC2 and CpbHLH13 transcription factors of wintersweet are possibly involved in the positive regulation and biosynthesis of monoterpene (linalool) and sesquiterpene (β-caryophyllene) in transgenic plants. This study also indicates the potential application of wintersweet as a valuable genomic material for the genetic modification of floral scent in other flowering plants that produce less volatile compounds.

scholarly journals Risk assessment of conventional crop plants in analogy to transgenic plants

1998 ◽  
Vol 5 (2) ◽  
pp. 89-93 ◽  
Author(s):  
Helge Torgersen ◽  
Gerhard Soja ◽  
Ines Janssen ◽  
Helmut Gaugitsch
Keyword(s):  
Risk Assessment ◽  
Transgenic Plants ◽  
Crop Plants

Construction of Two Vectors for a Bypass of Monocotyledon Plants Photorespiration

2011 ◽  
Vol 340 ◽  
pp. 351-356
Author(s):  
Xue Liang Bai ◽  
Dan Wang ◽  
Ning Ning Liu ◽  
Li Jing Wei ◽  
Ye Rong Zhu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Binary Vector ◽  
Transit Peptide ◽  
Expression Vectors ◽  
Chloroplast Transit Peptide ◽  
E Coli ◽  
Plant Expression ◽  
Glycolate Dehydrogenase ◽  
Save Energy

In order to modify the photorespiration of monocotyledonous crops, we aimed to construct vectors that will be used to introduce a bypass to the native photorespiration pathway. Firstly, we cloned the encoding sequences of glyoxylate carboligase (GCL) and tartronic semialdehyde reductase (TSR) fromE. coli, glycolate dehydrogenase (GDH) fromArabidopsis thalianaand chloroplast transit peptide (cTP) from rice. Then we constructed a universal vector pEXP harboring the encoding sequence of cTP for targeting a protein into chloroplast. By insertion of these three encoding sequences into the universal vector pEXP, we obtained the expression cassettes for GCL, TSR and GDH, respectively. Finally, we inserted the cassettes for GCL and TSR in tandem into the binary vector pCAMBIA 1301, and for GDH into another binary vector, pPGN, to obtain our plant expression vectors pCAMBIA 1301-TG and pPGN-GDH, respectively. These two expression vectors possess different selection resistance and can be used to transform monocots together, to introduce the bypass pathway of photorespiration. By this way, the transgenic plants can recycle glycolate, the by-product of photosynthesis in C3plants, within the chloroplast, simultaneously, save energy and avoid the loss of ammonia, which will contribute to improved growth.

scholarly journals Identification of reference genes for quantitative expression analysis using large-scale RNA-seq data of Arabidopsis thaliana and model crop plants

2016 ◽  
Vol 91 (2) ◽  
pp. 111-125 ◽  
Author(s):  
Toru Kudo ◽  
Yohei Sasaki ◽  
Shin Terashima ◽  
Noriko Matsuda-Imai ◽  
Tomoyuki Takano ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Expression Analysis ◽  
Reference Genes ◽  
Large Scale ◽  
Crop Plants ◽  
Rna Seq ◽  
Quantitative Expression ◽  
Model Crop

scholarly journals RNA interference-mediated tolerance to whitefly (Bemisia tabaci) in genetically engineered tomato

2021 ◽  
Author(s):  
Carolina Senhorinho Ramalho Pizetta ◽  
William Rafael Ribeiro ◽  
Amanda Lopes Ferreira ◽  
Matheus da Costa Moura ◽  
Kenny Bonfim ◽  
...  
Keyword(s):  
Rna Interference ◽  
Transgenic Plants ◽  
Bemisia Tabaci ◽  
Insect Pest ◽  
Genetically Engineered ◽  
Horticultural Crops ◽  
Transgenic Tomato Plants ◽  
Atpase Gene ◽  
Transgenic Lines ◽  
Polyphagous Insect

Abstract Whitefly (Bemisia tabaci) is a polyphagous insect that causes huge damage in several horticultural crops, including tomato, by sucking nutrients from the phloem and transmitting viruses. Whiteflies are particularly difficult to manage and the use of chemicals remains the common practice, which causes the development of insecticidal resistance. Thus, there is considerable interest in the introduction of whitefly resistance by classical and molecular breeding. Here, we explored the concept of using an RNA interference construct to silence a v-ATPase gene in whiteflies interacting with transgenic tomato plants that express siRNA molecules corresponding to a fragment from the B. tabaci vATPase. PCR analyses revealed the presence of both ΔATPase and nptII transgenes in all transgenic lines. siRNA expressing lines were challenged against whitefly and revealed a mortality rate of 57.1% in transgenic line 4.4.1, while in the control the mortality was 7.6%. Mortality of 2nd instar nymphs was higher on the transgenic plants and the development of 3rd instar nymphs was slightly longer than on the control plants. Although the attraction of insects was not significantly different between treatments, the number of eggs laid by the insects on the transgenic plants was significantly lower, compared to the controls. RT-qPCR revealed a decreased expression level of endogenous v-ATPase gene in whiteflies feeding on transgenic plants. No unexpected effect was observed on the non-target insects Myzus persicae or Tuta absoluta. Results presented here may form the foundation for the generation of elite tomato varieties resistant to whitefly, a devastating insect pest.

Sign in / Sign up

Export Citation Format

Share Document