Interactions between Clinicians and the Microbiology Laboratory

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly. Keywords: Green betel leaf extract , organic insecticide, houseflies

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Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v1i2.21506 ◽

2016 ◽

Vol 1 (2) ◽

pp. 38-42 ◽

Cited By ~ 4

Author(s):

Khairun Nessa ◽

Dilruba Ahmed ◽

Johirul Islam ◽

FM Lutful Kabir ◽

M Anowar Hossain

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Clinical Laboratory ◽

Proteinase K ◽

Microbiology Laboratory ◽

Pcr Assay ◽

E Coli ◽

Diarrheagenic Escherichia Coli ◽

Multiplex Pcr Assay ◽

Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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Prevalence of Panton-Valentine Leukocidin (PVL) Gene among Staphylococcus Aureus Isolated from Environmental Surface of working area in Microbiology Laboratory

10.33329/aus.2019.n26.4.28 ◽

2019 ◽

Vol 26 (4) ◽

pp. 201-205

Keyword(s):

Staphylococcus Aureus ◽

Microbiology Laboratory ◽

Environmental Surface ◽

Panton Valentine Leukocidin ◽

Valentine Leukocidin

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Implications of rDNA technology in the microbiology laboratory

Pathology ◽

10.3109/00313029209063175 ◽

1992 ◽

Vol 24 (3) ◽

pp. 205-210

Author(s):

Ronald J. Trent ◽

Colin MacLeod

Keyword(s):

Microbiology Laboratory

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Assessment of workload in the microbiology laboratory

Pathology ◽

10.3109/00313027709084811 ◽

1977 ◽

Vol 9 (3) ◽

pp. 207-212 ◽

Cited By ~ 1

Author(s):

V.P. Ackerman ◽

P.S. Berriman

Keyword(s):

Microbiology Laboratory

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Ohio water microbiology laboratory

Fact Sheet ◽

10.3133/fs20053072 ◽

2005 ◽

Cited By ~ 1

Author(s):

Keyword(s):

Microbiology Laboratory ◽

Water Microbiology

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Decrease in Enteroviral Meningitis - An Unexpected Benefit of COVID-19 Mitigation?

Clinical Infectious Diseases ◽

10.1093/cid/ciaa1881 ◽

2020 ◽

Author(s):

Kami D Kies ◽

Amber S Thomas ◽

Matthew J Binnicker ◽

Kelli L Bashynski ◽

Robin Patel

Keyword(s):

Cerebrospinal Fluid ◽

Laboratory Testing ◽

Clinical Microbiology ◽

Late Summer ◽

Mitigation Strategies ◽

Clinical Microbiology Laboratory ◽

Microbiology Laboratory ◽

Testing Data ◽

Early Fall ◽

Enteroviral Meningitis

Abstract Enteroviral meningitis is seasonal, typically exhibiting a rise in prevalence in late summer/early fall. Based on clinical microbiology laboratory testing data of cerebrospinal fluid, the expected August/September/October peak in enteroviral meningitis did not occur in 2020, possibly related to COVID-19 mitigation strategies.

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Implementation and evaluation of a novel real-time multiplex assay for SARS-CoV-2: in-field learnings from a clinical microbiology laboratory

Pathology ◽

10.1016/j.pathol.2020.08.004 ◽

2020 ◽

Vol 52 (7) ◽

pp. 754-759 ◽

Cited By ~ 2

Author(s):

Eloise Williams ◽

Katherine Bond ◽

Brian Chong ◽

Dawn Giltrap ◽

Malcolm Eaton ◽

...

Keyword(s):

Real Time ◽

Clinical Microbiology ◽

Multiplex Assay ◽

Clinical Microbiology Laboratory ◽

Microbiology Laboratory

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The Potential of Endophytic Fungi Isolated from Leaves, Stems, Mangrove Roots Avicennia marina as a Producer of Antibiotics

Metamorfosa Journal of Biological Sciences ◽

10.24843/metamorfosa.2019.v06.i01.p13 ◽

2019 ◽

Vol 6 (1) ◽

pp. 83

Author(s):

Kustiasih Lestari ◽

Anthoni Agustien ◽

Akmal Djamaan

Keyword(s):

Endophytic Fungi ◽

Inhibition Zone ◽

Avicennia Marina ◽

Antibiotic Activity ◽

Microbiology Laboratory ◽

Inhibitory Zone ◽

Fungal Isolation ◽

Activity Test ◽

Mangrove Roots ◽

Source Of Information

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.

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