The Potential of Endophytic Fungi Isolated from Leaves, Stems, Mangrove Roots  Avicennia marina as a Producer of Antibiotics

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.

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UJI AKTIVITAS ANTIBAKTERI JAMUR ENDOFIT AKAR BAKAU Avicennia marina TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli

Jurnal e-Biomedik ◽

10.35790/ebm.2.1.2014.3750 ◽

2014 ◽

Vol 2 (1) ◽

Author(s):

Firdy Liwang

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Endophytic Fungi ◽

Inhibition Zone ◽

Avicennia Marina ◽

Positive Control ◽

Potato Dextrose Agar ◽

Fungal Mycelium ◽

Tidal Zone ◽

E Coli

Abstract: In this his study we used endophytic fungi isolated from the roots of mangrove Avicennia marina growing on tidal zone around Tasik Ria Minahasa, North Sulawesi. The fungi were isolated and then tested the antibacterial effect against Staphylococcus aureus and Escherichia coli. Potato Dextrose agar was used in order to isolate the target fungi. The fungi began to grow on the second day after inoculation. Differentiation and purification processes to isolate the fungus obtained by observing fungi mycelia. While, the antibacterial activity test was following agar diffusion assay by planting 10 mm diameter of fungal mycelium together with particular bacteria onto the combination media. Ciprofloxacin was used as a positive control. The results shows a visible bacterial inhibition zone around the growing fungi with a diameter between 18 mm to 20.4 mm. Based on the results, it can be concluded that endophytic fungi isolated from the roots of mangrove Avicennia marina is able to inhibit the growth of S.aureus and E. coli. Keyword: Avicennia marina, Antibacterial, endophytic fungi Abstrak: Penelitian ini menggunakan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina yang tumbuh di perairan sekitar Tasik Ria Minahasa, Sulawesi Utara. Jamur endofit yang diisolasi kemudian diuji efek antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Isolasi jamur endofit menggunakan media PDA (Potato Dextrose Agar). Jamur mulai tumbuh pada hari kedua setelah inokulasi. Proses pemurnian jamur diperoleh dengan mengisolasi jamur dengan membedakan miselia. Pengujian efek antibakteri mengikuti prosedur agar difusi dengan menanam miselium jamur sebesar 10 mm pada media kombinasi yang telah diolesi bakteri uji. Sebagai kontrol positif digunakan Ciprofloxacin. Hasil penelitian terlihat diameter zona hambat pertumbuhan bakteri yang terbentuk sebesar 18 mm hingga 20,4 mm. Berdasarkan hasil penelitian maka dapat disimpulkan jamur endofit yang diisolasi dari akar tumbuhan bakau Avicennia marina mampu menghambat pertumbuhan bakteri S. aureus dan bakteri E. coli. Kata kunci: Avicennia marina, antibakteri, jamur endofit

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Endophytic Fungi Isolated from Rhizophora mucronata and Their Antibacterial Activity

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v8i2.88 ◽

2013 ◽

Vol 8 (2) ◽

pp. 69 ◽

Cited By ~ 1

Author(s):

Kustiariyah Tarman ◽

Dwi Safitri ◽

Iriani Setyaningsih

Keyword(s):

Antibacterial Activity ◽

Endophytic Fungi ◽

Inhibition Zone ◽

Culture Broth ◽

Rhizophora Mucronata ◽

Economic Potential ◽

Mangrove Plant ◽

Traditional Medicines ◽

Activity Test ◽

Fungal Extracts

Mangrove plants are known as source of traditional medicines. Endophytic fungi have an important economic potential for antibacterial, enzyme and secondary metabolites that are useful especially in pharmaceutical industry. The purpose of this research was to determine the antibacterial activity of the endophytic fungal extracts from mangrove plant Rhizophora mucronata against diarrhea causing bacteria. This research included isolation, characterization, cultivation, extraction, and antibacterial activity test. The study yielded 5 types of endophytic fungi with different morphology. Endophytic fungus DS1 was the selected isolate based on antagonism test. The growth pattern showed the stationary phase of isolate DS1 was on the 15th day. Culture broth extract showed considerable inhibition on Pseudomonas aeruginosa with diameter of inhibition zone was 18.5 mm ± 3.32. Extract of mycelium inhibited the growth of Staphylococcus aureus (2 mm). The culture broth extract showed more potent antibacterial activity in comparison with the mycelium extract.

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UJI EFEK ANTIBAKTERI JAMUR ENDOFIT DAUN MANGROVE AVICENNIA MARINA TERHADAP BAKTERI UJI STAPHYLOCOCCUS AUREUS DAN SHIGELLA DYSENTERIAE

Jurnal e-Biomedik ◽

10.35790/ebm.3.1.2015.6632 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Yolanda A. Kasi ◽

Jimmy Posangi ◽

O. Mona Wowor ◽

Robert Bara

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Endophytic Fungi ◽

Antibacterial Effect ◽

Avicennia Marina ◽

Shigella Dysenteriae ◽

Plant Tissues ◽

Mangrove Plant ◽

Activity Test ◽

White Mycelium

Abstract: Endophytic fungi is a species of fungi which lives within plant tissues including leaf’s tissues that also harmless for the host plant itself. Isolates of endophytic fungi have a potent antibacterial capability with broad spectrum. The aim of this research is to know whether there is an antibacterial effect of endophytic fungi which isolated from Avicennia marina leaf towards Staphylococcus aureus and Shigella dysenteriae. The method that is used for the antibacterial activity test, done by putting mycelia of endophytic fungi in combination media that has been smeared with Staphylococcus aureus and Shigella dysenteriae. Based on research that has been done, it obtained two types of endophytic fungi isolated from mangrove plant, Avicennia marina leaf. Both endophytic fungi have potential for inhibiting Staphylococcus aureus and Shigella dysenteriae, but endophytic fungi with white mycelium has a better antibacterial effect than endophytic fungi with black mycelium and positive control.Keywords: antibacterial, endophytic fungi, mangrove avicennia marinaAbstrak: Jamur endofit merupakan jamur yang hidup di dalam jaringan tanaman salah satunya yaitu dalam jaringan daun dan tidak membahayakan tanaman tersebut. Isolat-isolat jamur endofit memiliki daya antibakteri yang poten dengan spektrum luas. Penelitian ini bertujuan untuk mengetahui ada tidaknya efek antibakteri jamur endofit daun Avicennia marina terhadap bakteri Staphylococcus aureus dan Shigella dysenteriae. Metode yang digunakan untuk uji antibakteri yaitu dilakukan dengan cara menempelkan miselia jamur endofit pada media agar kombinasi yang telah dioleskan bakteri uji. Dari penelitian yang dilakukan, diperoleh dua jenis jamur endofit yang diisolasi dari daun Mangrove Avicennia marina. Kedua jamur endofit memiliki potensi dalam menghambat kedua bakteri uji tetapi jamur endofit miselium putih memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit miselium hitam dan kontrol positif.Kata kunci: antibakteri, jamur endofit, daun avicennia marina

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Skrining Fitokimia Dan Aktivitas Antimikroba Dari Tumbuhan Bangun-Bangun (Coleus Amboinicus Lour)

Talenta Conference Series Science and Technology (ST) ◽

10.32734/st.v2i1.322 ◽

2019 ◽

Vol 2 (1) ◽

pp. 92-96

Author(s):

Rosliana Lubis

Keyword(s):

Pseudomonas Aeruginosa ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Compounds ◽

Inhibition Zone ◽

Inhibitory Zone ◽

E Coli ◽

Activity Test ◽

In Vitro Measurements

Penelitian ini bertujuan untuk mengekstraksi, skrining fitokimia, dan analisa aktivitas mikroba dari daun bangun-bangun dengan menggunakan pelarut etil asetat 70% dan n-heksan. Metode ekstraksi menggunakan metode maserasi. Komposisi kimia senyawa aktif yang diuji terdiri dari alkaloid, flavonoid, terpenoid, tanin, dan saponin. Aktivtias antimikroba ekstrak etil asetat 70% dilakukan menggunakan metode pengukuran luas zona hambat. Bakteri uji yang digunakan terdiri dari E. Coli ATCC 25922 dan Pseudomonas aeruginos. Uji aktivitas bakteri dilakukan bersifat invitro. Pengukuran zona hambat menggunakan variasi konsentrasi ekstrak etil asetat 70% terdiri dari 0%, 15%, 30%, 45%, 60%, dan 75% (v/v). Hasil Penelitian :menunjukkan bahwa ekstrak etil asetat 70% daun Handeuleum secara kualitatif teridentifikasi memilki kandungan senyawa kimia alkaloid, Flavonoid, Terpenoid, Steroid, Tanin, dan Saponin. Aktivitas bakteri E. Coli ATCC 25922 dan Pseudomonas aeruginosa terhadap ekstrak didasarkan kepada pengukuran luas zona hambat yang terbentuk. Luas zona hambat yang terbentuk dari ekstrak etil asetat daun Handeuleum dengan range konsentrasi 0% s.d 75 % terdiri dari 9,50 mm – 21,2 mm dan 9,00 mm – 20,6 mm. This study aimed to extract, phytochemically screen, and analyze the microbial activity of bangun-bangun leaves using 70% ethyl acetate and n-hexane solvents. The extraction method used was maceration method. The chemical composition of the active compounds tested consisted of alkaloids, flavonoids, terpenoids, tannins, and saponins. The antimicrobial activity of 70% ethyl acetate extract was carried out using the method of measuring the inhibitory zone. The bacteria used consisted of E. coli ATCC 25922 and Pseudomonas aeruginos. The bacterial activity test was in vitro. Measurements of inhibitory zones using variations in the concentration of 70% ethyl acetate extract consisted of 0%, 15%, 30%, 45%, 60% and 75% (v / v). The results showed that 70% of the ethyl acetate extract of Handeuleum leaves were qualitatively identified as containing alkaloid chemical compounds, Flavonoids, Terpenoids, Steroids, Tanins, and Saponins. The activities of E. coli bacteria ATCC 25922 and Pseudomonas aeruginosa towards the extracts were based on measurements of the area of inhibition zone formed. The area of inhibition zone formed from the ethyl acetate extract of Handeuleum leaves were with a concentration range of 0% to 75% consisting of 9.50 mm - 21.2 mm and 9.00 mm - 20.6 mm.

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Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.38138 ◽

2018 ◽

Vol 3 (3) ◽

pp. 85

Author(s):

Novi Permata Sari ◽

Rafika Sari ◽

Eka Kartika Untari

Keyword(s):

Antibacterial Activity ◽

Lactobacillus Plantarum ◽

Lactobacillus Casei ◽

Pathogenic Bacteria ◽

Heating Temperature ◽

Lactobacillus Brevis ◽

Inhibition Zone ◽

Diffusion Method ◽

Activity Test ◽

Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum.

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Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

Natural Product Communications ◽

10.1177/1934578x1601100941 ◽

2016 ◽

Vol 11 (9) ◽

pp. 1934578X1601100

Author(s):

Sakon Monggoot ◽

Jariya Burawat ◽

Patcharee Pripdeevech

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Endophytic Fungi ◽

Ethyl Acetate Extract ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

High Concentration ◽

Phenyl Ethyl Alcohol ◽

First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

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Identification of Endophytic Fungi from Rhizome Galanga (Alpinia galanga L.) against Candida albicans

Metamorfosa: Journal of Biological Sciences ◽

10.24843/metamorfosa.2018.v05.i02.p10 ◽

2018 ◽

Vol 5 (2) ◽

pp. 205

Author(s):

Ni Made Raningsih ◽

Putu Wahyu Sri Juniantari Sandy

Keyword(s):

Candida Albicans ◽

Endophytic Fungi ◽

Trichoderma Harzianum ◽

Food Microbiology ◽

Microbiology Laboratory ◽

Isolation And Identification ◽

Alpinia Galanga

The main objectives of this research were to isolat and identify endophytic fungi antagonists of Candida albicnas from galanga rhizomes. Samples of galanga rhizome were collected from Gitgit plantation in Gitgit village, Buleleng regency. Isolation and identification were conducted in Food Microbiology Laboratory, Faculty of Agriculture, Udayana University. There were three isolates identified with inhibitory persentage againts Candida albicans i.e. Trichoderma viridae (26.07±3.76%), Trichoderma harzianum (45.11±5.84%) and Fusarium oxyforum (20.11±5.61%).

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THE ANTIBACTERIAL ACTIVITY OF FRACTIONS OF ETHYL ACETATE GARCINIA LATISSIMA MIQ. STEM BARK EXTRACTS AGAINST BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s5.23100 ◽

2017 ◽

Vol 10 (17) ◽

pp. 69

Author(s):

Neneng Sitisilfi Ambarwati ◽

Amarila Malik ◽

Elfira Amalia Deborah ◽

Cakra Hagai Arpatism ◽

Maulfi Hanif ◽

...

Keyword(s):

Bacillus Subtilis ◽

Ethyl Acetate ◽

Silica Gel ◽

Mobile Phase ◽

Agar Medium ◽

Inhibition Zone ◽

Stem Bark ◽

Tetrazolium Salt ◽

Inhibitory Zone ◽

Zone Diameter

Objective: This research focuses on the antibacterial activities of fractions of ethyl acetate Garcinia latissima Miq. extracts against Bacillus subtilis and Pseudomonas aeruginosa.Methods: For each bacteria, the values of the inhibitory zone diameter were determined using paper discs method, while the minimum inhibitory concentration (MIC) was determined by microdilution and antibacterial assay was determined by bioautography. Fractionation was carried out using gradient elution with silica gel column as stationary phase successively increasing the separation according to the polarity (using combination of eluents; n-hexane, ethyl acetate, and methanol).Results: To determine the diameter of the inhibitory zone for each fraction, the fractions were diluted with dimethyl sulfoxide until the concentration of each fraction was 20,000 ppm. The results showed that fraction B had no inhibitory zone against B. subtilis. The inhibitory zone diameters of fractions A, C, D, E, F, and G against B. subtilis were 7.600±0.000, 6.767±0.202, 7.950±0.477, 7.883±0.901, and 9.233±0.231 mm, respectively. Only Fraction G had an inhibition zone diameter of 7.200±0.173 mm against P. aeruginosa. The active fractions were tested by contact bioautography using silica gel (60 GF254) plate and hexane:chloroform (2:3) as mobile phase for Fractions A, C, D, E, and F and chloroform:ethyl acetate (4:1) as mobile phase for Fraction G. The thin layer chromatography plates were observed under the ultraviolet light at wavelengths of 254 and 366 nm, while the others plate (using in contact bioautography) placing on agar medium that had been inoculated with the bacteria, so the compounds could be diffuse to the medium. The active compound will appeared inhibitory zones in agar medium. The determination of the MIC values using microdilution with methylthiazol tetrazolium salt showed that fractions of the ethyl acetate extracts of G. latissima Miq. stem bark inhibited the growth of B. subtilis more actively than P. aeruginosa. The highest activity of the fractions against B. subtilis was shown by fraction G, which had a MIC value of 78.125 ppm, followed by fractions F (156.25 ppm), E (312.5 ppm), D (625 ppm), C (1250 ppm), B (2500 ppm), and A (5000 ppm). The highest activity of the fractions against P. aeruginosa was Fraction G (5000 ppm), while the MIC values of other fractions were >5000 ppm.Conclusion: Further investigations should be conducted to obtain the new antibacterial compounds in fractions of ethyl acetate extract of G. latissima Miq. stem bark that effective to inhibit the growth of B. subtilis and P. aeruginosa.

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Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.840.205 ◽

2020 ◽

Vol 840 ◽

pp. 205-213

Author(s):

Elfita Elfita ◽

Muharni Muharni ◽

Mardiyanto Mardiyanto ◽

Fitrya Fitrya ◽

Feti Fera ◽

...

Keyword(s):

Antibacterial Activity ◽

Endophytic Fungi ◽

Bacterial Infections ◽

Salmonella Typhi ◽

Spectroscopy Analysis ◽

Chromatography Method ◽

Antibacterial Compounds ◽

Activity Test ◽

Antibacterial Compound ◽

Promising Source

Scurrula atropurpurea known as benalu is a medicinal plant that has been used for the treatment of various diseases such as antibacterial. Plants with ethnomedicine history use to cure pathogenic bacterial infections and their endophytic fungi is a promising source of antibacterial compounds. This study aimed to compared the antibacterial activity of S. atropurpurea leaves and their endophytic fungi. The secondary metabolites were isolated from the leaves of S. atropurpurea and their endophytic fungi by the chromatography method. The antibacterial activity test was carried out by Kirby Bauer method against Salmonella typhi (IPCCCB.11.669) and Escherichia coli (ATCC 25922) as Gram (-) and Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) as Gram (+). The antibacterial compound from S. atropurpurea was determined by spectroscopy analysis as Quercetin-3-O-α-L-Rhamnopyranoside, while the antibacterial compound from endophytic fungi (strain BB1) as a lactone. Phylogenetic tree of strain BB1 has the highest homology with Neopestalotiopsis surinamensis strain CBS 450.74.

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Potensi Daun Teh (Camellia sinensis) dan Daun Anting-anting Acalypha indica L. dalam Menghambat Pertumbuhan Salmonella typhi

JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI ◽

10.36722/sst.v2i2.131 ◽

2013 ◽

Vol 2 (2) ◽

pp. 104

Author(s):

Nita Noriko

Keyword(s):

Camellia Sinensis ◽

Antibacterial Agent ◽

Leaf Extract ◽

Bacterial Resistance ◽

Inhibition Zone ◽

Salmonella Typhi ◽

The Body ◽

Microbiology Laboratory ◽

Clear Zone ◽

Acalypha Indica

Abstrak - Salmonella typhi adalah bakteri penyebab terjadinya penyakit typhus. Pengobatan menggunakan antibiotika menimbulkan resistensi jika dilakukan tidak tuntas terutama ketika sistem tubuh menurun. Pengobatan dengan menerapkan kearifan lokal merupakan salah satu alternatif dalam upaya penyembuhan. Daun teh (Camellia sinensis) dan daun anting-anting (Acalypha indica L) diduga mengandung tanin, suatu senyawa yang dapat berfungsi sebagai antibiotik. Tujuan dari penelitian ini adalah memperoleh informasi efektifitas ekstrak daun teh dan daun anting-anting sebagai antibakteri alami, khususnya S. typhi Penelitian dilakukan pada bulan Maret hingga Oktober 2012 di Laboratorium Mikrobiologi Universitas Al Azhar Indonesia. Tahapan penelitian terdiri dari pembuatan ekstrak daun teh dan anting-anting, pengujian adanya kandungan tanin, penumbuhan S. typhi dan uji antibakteri. Ekstrak daun diperoleh dengan cara perebusan dan pengeringan. Hasil penelitian menunjukkan bahwa ekstrak daun teh dan anting-anting berpotensi untuk dikembangkan sebagai antibiotik. Hal ini ditunjukkan dengan terbentuknya zona bening ketika dilakukan uji antibakteri. Kedua ekstrak daun tersebut positif mengandung tanin yang diduga berperan dalam penghambatan pertumbuhan S. typhi. Abstract - Salmonella typhi is a bacteria that caused typhus disease. The curation using chemical drugs may cause bacterial resistance if the treatment is not complete, especially when the system of the body decreases. Treatment with traditional medicine is one of alternative way for curing typhoid fever. Tea and Anting-anting leaves are suspected to contain tannin, a compound that can act as an antibiotic. The aim of the research is getting information about effectiveness of tea and anting-anting leaf extract as antibacterial agent specially S. thypi. Research were conducted from Maret until October 2012 in Microbiology Laboratory, Al Azhar Indonesia University. The steps incude collecting tea and anting-anting leaves, extracting tannin, testing for tannin, growing the bacteria, and testing the inhibition zone. Leaf extract obtained by boiling and drying. The research shows that leaf tea and anting-anting extraction have potential to be developed as an antibiotic. That are showed by clear zone as indicate inhibition Salmonella typhi when tested antibacterial. Both of the leaves plant are positive tannin containing, and it is indicated as inhibitor S. thypi growth.

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