Optimization of Protein Array Fabrication for Establishing High-Throughput Ultra-Sensitive Microarray Assays for Cancer Research

2009 ◽  
pp. 289-299
Author(s):  
Gary Hardiman ◽  
C Bhasker ◽  
Richard Rouse
Keyword(s):  
Cancer Research ◽  
High Throughput ◽  
Protein Array ◽  
Array Fabrication

Utility of High-Throughput Imaging Mass Cytometry for Cancer Research: A feasibility study

2021 ◽  
Author(s):  
Sindhura Thirumal ◽  
Amoon Jamzad ◽  
Tiziana Cotechini ◽  
Charles T. Hindmarch ◽  
Celine Hardy ◽  
...  
Keyword(s):  
Cancer Research ◽  
Feasibility Study ◽  
High Throughput ◽  
Mass Cytometry ◽  
High Throughput Imaging

scholarly journals Organoid Models for Cancer Research

2019 ◽  
Vol 3 (1) ◽  
pp. 223-234 ◽  
Author(s):  
Hans Clevers ◽  
David A. Tuveson
Keyword(s):  
Cancer Research ◽  
Tumor Microenvironment ◽  
Personalized Medicine ◽  
High Throughput ◽  
Stromal Cells ◽  
Transcriptome Sequencing ◽  
Cancer Biology ◽  
Three Dimensional ◽  
Model Systems ◽  
Liver Cancers

Organoid cultures have emerged as powerful model systems accelerating discoveries in cellular and cancer biology. These three-dimensional cultures are amenable to diverse techniques, including high-throughput genome and transcriptome sequencing, as well as genetic and biochemical perturbation, making these models well suited to answer a variety of questions. Recently, organoids have been generated from diverse human cancers, including breast, colon, pancreas, prostate, bladder, and liver cancers, and studies involving these models are expanding our knowledge of the etiology and characteristics of these malignancies. Co-cultures of cancer organoids with non-neoplastic stromal cells enable investigation of the tumor microenvironment. In addition, recent studies have established that organoids have a place in personalized medicine approaches. Here, we describe the application of organoid technology to cancer discovery and treatment.

Detection of breast cancer-related antigens through cDNA phage-displayed protein microarray

2010 ◽  
Vol 391 (7) ◽  
Author(s):  
Qun Bi ◽  
Taochao Tan ◽  
Xi Xiang ◽  
Aiping Lu ◽  
Shenggeng Zhu
Keyword(s):  
Breast Cancer ◽  
Cancer Research ◽  
High Throughput ◽  
Breast Tumor ◽  
Protein Microarray ◽  
Molecular Profiling ◽  
Multifactorial Disease ◽  
Cdna Sequences ◽  
Profiling Techniques ◽  
Related Proteins

AbstractHigh-throughput molecular profiling techniques are helpful in the diagnosis of multifactorial disease. In this study, a cDNA-phage-displayed protein microarray using phage particles spotted directly onto it as sensors was used to detect related antigens in breast tumor sera. cDNA sequences from 17 positive clones were determined, which included some sequences encoding known breast cancer-related antigens and proteins related to other diseases, as well as proteins with unknown functions. Our results not only provide some useful information for breast cancer research, but also suggest that the strategy used here would be efficient to search for disease-related proteins and other functional target proteins.

scholarly journals The motivations and methodology for high-throughput PET imaging of small animals in cancer research

2012 ◽  
Vol 39 (9) ◽  
pp. 1497-1509 ◽  
Author(s):  
Nicolas Aide ◽  
Eric P. Visser ◽  
Stéphanie Lheureux ◽  
Natacha Heutte ◽  
Istvan Szanda ◽  
...  
Keyword(s):  
Cancer Research ◽  
High Throughput ◽  
Pet Imaging ◽  
Small Animals

DNA-LINKED PROTEIN ARRAY FOR HIGH-THROUGHPUT PROTEOMICS: FROM SPATIALLY UNKNOWN DNA ARRAYS TO IDENTIFIABLE PROTEIN ARRAYS

Nano LIFE ◽  
2010 ◽  
Vol 01 (01n02) ◽  
pp. 33-43 ◽  
Author(s):  
MANISH BIYANI ◽  
NAOTO NEMOTO ◽  
TAKANORI ICHIKI
Keyword(s):  
High Throughput ◽  
Protein Array ◽  
Functional Proteomics ◽  
Functional Assay ◽  
Dna Arrays ◽  
Protein Fusion ◽  
Protein Arrays ◽  
Pou Domain ◽  
Domain Protein ◽  
One To One

Protein array technology has a tremendous potential in large-scale and high-throughput functional proteomics. The conventional protein array approach, which is based on one-to-one indexing between a known protein and an array position, is however, not applicable for decoding genetic information from unknown expressed protein arrays and thus less practicable for global proteome analysis. To address this issue, we have introduced a novel concept of DNA-linked protein array by adopting a strategy of one-to-one indexing between spatially unknown individual DNA arrays and their encoded protein array products. A set of test DNA arrays of DNA-binding (POU) domain protein, c-Myc-tagged POU domain protein, and green fluorescent protein were randomly patterned on a glass surface and subsequently converted into identifiable protein arrays by simultaneously producing and linking the encoded proteins to their encoding genes using a puromycin-assisted mRNA–protein fusion approach in situ. For concept validation, functional assay was performed by the screening of mRNA–protein fusion arrays against a mixture of anti-cMyc and anti-GFP antibodies. Moreover, this approach also provides an additional advantage to incorporate post-translational modifications into protein arrays. The concept developed here is expected to markedly enhance the application of protein microarrays to the study of functional proteomics.

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