Prolactin Releasing Peptide (PrRP) Stimulates Luteinizing Hormone (LH) and Follicle Stimulating Hormone (FSH) via a Hypothalamic Mechanism in Male Rats

SUMMARY The effects of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on plasma testosterone levels were examined in hypophysectomized and in intact immature and adult male rats. The animals were injected with saline, LH, FSH, or both gonadotrophins twice daily for 3·5 days and were killed 3 h after the last injection. Plasma testosterone levels were measured by radioimmunoassay. In immature hypophysectomized rats, plasma testosterone levels were not changed by treatment with LH, FSH or LH plus FSH. The weight of the testes and of the seminal vesicles was increased only in animals injected with both LH and FSH. In adult hypophysectomized rats, LH caused the expected increase in plasma testosterone levels, while FSH injected alone had no effect. Plasma testosterone levels in rats treated with 5 μg LH and 20 μg FSH were significantly greater than those in animals given 5 μg LH alone. However, the same dose of FSH did not potentiate the action of 25 μg LH on plasma testosterone levels. In adult hypophysectomized rats the weight of testes was not affected by any of the treatments. The weight of the seminal vesicles was increased by the higher dose of LH and addition of FSH caused no further increase. In intact immature and adult rats plasma testosterone levels and the weight of testes were not changed by any of the treatments. Seminal vesicle weight was increased only in adult rats treated with the higher dose of LH together with FSH. The results demonstrate that FSH potentiates the action of low doses of LH on plasma testosterone levels in adult hypophysectomized rats and suggest that FSH may be involved in the regulation of androgen secretion by the rat testis.

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Effect of Active and Passive Immunization with Luteinizing Hormone-Releasing Hormone on Serum LuteinizingHormone and Follicle-Stimulating Hormone Levels nd the Ultrastructure of the Pituitary Gonadotrophs in Castrated Male Rats

Endocrinology ◽

10.1210/endo-99-1-291 ◽

1976 ◽

Vol 99 (1) ◽

pp. 291-303 ◽

Cited By ~ 27

Author(s):

A. ARIMURA ◽

M. SHINO ◽

K. G. DE LA CRUZ ◽

E. G. RENNELS ◽

A. V. SCHALLY

Keyword(s):

Luteinizing Hormone ◽

Follicle Stimulating Hormone ◽

Passive Immunization ◽

Male Rats ◽

Luteinizing Hormone Releasing Hormone ◽

Releasing Hormone ◽

Hormone Levels ◽

Stimulating Hormone

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A Quantitative Immunocytochemical Study of the Luteinizing Hormone and Follicle-Stimulating Hormone Cells in the Adenohypophysis of Adult Male Rats and Adult Female Rats throughout the Estrous Cycle*

Endocrinology ◽

10.1210/endo-113-3-970 ◽

1983 ◽

Vol 113 (3) ◽

pp. 970-984 ◽

Cited By ~ 37

Author(s):

M. O. DADA ◽

G. T. CAMPBELL ◽

C. A. BLAKE

Keyword(s):

Luteinizing Hormone ◽

Estrous Cycle ◽

Adult Female ◽

Adult Male ◽

Follicle Stimulating Hormone ◽

Female Rats ◽

Male Rats ◽

Immunocytochemical Study ◽

Stimulating Hormone

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Increase in Pituitary Levels of Luteinizing Hormone and Follicle-Stimulating Hormone after Pinealectomy in Both Intact and Castrated Male Rats

Endocrinology ◽

10.1210/endo-102-5-1534 ◽

1978 ◽

Vol 102 (5) ◽

pp. 1534-1538 ◽

Cited By ~ 4

Author(s):

RAFAEL ALONSO ◽

LUIS PRIETO ◽

MANUEL MAS

Keyword(s):

Luteinizing Hormone ◽

Follicle Stimulating Hormone ◽

Male Rats ◽

Stimulating Hormone

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24-Hour Changes in Circulating Prolactin, Follicle-Stimulating Hormone, Luteinizing Hormone, and Testosterone in Young Male Rats Subjected to Calorie Restriction

Chronobiology International ◽

10.1081/cbi-120038607 ◽

2004 ◽

Vol 21 (3) ◽

pp. 393-404 ◽

Cited By ~ 19

Author(s):

Fernando Chacon ◽

Pilar Cano ◽

Vanessa Jimenez ◽

Daniel P. Cardinali ◽

Ascensión Marcos ◽

...

Keyword(s):

Luteinizing Hormone ◽

Calorie Restriction ◽

Follicle Stimulating Hormone ◽

Young Male ◽

Male Rats ◽

Stimulating Hormone

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Effects of Satureja Edmondi Briq on Ameliorate Reproductive Parameters in Pentylenetetrazol-Induced Epilepsy Models in Rats

10.21203/rs.3.rs-93397/v1 ◽

2020 ◽

Author(s):

somayeh bohlouli ◽

gelavij mahmoodi

Keyword(s):

Luteinizing Hormone ◽

Essential Oil ◽

Sperm Count ◽

Follicle Stimulating Hormone ◽

Reproductive Potential ◽

Sperm Parameters ◽

Male Rats ◽

World Health ◽

Health Organization ◽

Stimulating Hormone

Abstract BackgroundEpilepsy is one of the common neurological brain defects that causes unpredictable, recurrent seizures. It has adverse effects on the reproductive functions.Oxidative stress contributes to the evolution of epilepsy, including reduction of sperm count and motility,increase of abnormal sperm morphology, and low testosterone.Antioxidants are beneficial to spermatogenesis and sperm parameters. Satureja edmondiBriq is known as a powerful antioxidant that can reduce the effects of oxidative stress. The aim of this study was to evaluate the effects of Satureja edmondion reproductive potential in pentylenetetrazol (PTZ)-induced epileptic male rats.MethodsIn this experimental study, 48 Wistar male rats with an average age of 10 weeks and weightof 240- 260g were used. Animals were randomized into normal and kindled groups that were treated with different doses of S.edmondi essential oil. Treatment of animals lasted 4 weeks. In the end, testosterone, follicle-stimulating hormone, luteinizing hormone, and sperm parameters were measured according to the World Health Organization (WHO) standards.ResultsThis study showed that all sperm parameters,including sperm count, sperm viability, and progressive sperm motility were increased and testosterone, follicle-stimulating hormone and luteinizing hormone (FSH,LH) changes in the epileptic and normal groups were changed with an increase in the dose of S.edmondi essential oil. ConclutionsThe changes were significant in some parameters. S.edmondi as a good source of antioxidants can improve sperm parameters and reproductive potential in PTZ-induced epileptic male rats.

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Recombinant forms of rat and human luteinizing hormone and follicle-stimulating hormone; comparison of functions in vitro and in vivo

Journal of Endocrinology ◽

10.1677/joe.0.1580441 ◽

1998 ◽

Vol 158 (3) ◽

pp. 441-448 ◽

Cited By ~ 14

Author(s):

K Hakola ◽

AM Haavisto ◽

DD Pierroz ◽

A Aebi ◽

A Rannikko ◽

...

Keyword(s):

Luteinizing Hormone ◽

Cell Line ◽

Follicle Stimulating Hormone ◽

Tumor Cell Line ◽

Male Rats ◽

Rat Pituitary ◽

Functional Features ◽

Stimulating Hormone

We have previously described the preparation, purification and partial characterization of recombinant (rec) forms of rat luteinizing hormone (LH) and follicle-stimulating hormone (FSH). In the present study, the special functional features of these hormones were studied further, in vitro and in vivo, and compared with human recLH and recFSH, as well as with human urinary choriongonadotropin (hCG) and rat pituitary LH (NIDDK-RP3). In radioreceptor assay, the affinity of hCG binding to rat testis membranes was 5-fold higher than that of human recLH and 100-fold higher than that of rat recLH. In in vitro bioassay, using dispersed adult mouse interstitial cells or a mouse Leydig tumor cell line (BLT-1), hCG and human recLH were 10- to 20-fold more potent than rat recLH. Correspondingly, rat pituitary LH was about 10-fold less potent than rat recLH, and evoked a maximum testosterone response that was about half of that elicited by the other LH/CG preparations. Rat recFSH was about 10-fold less potent than human recFSH in stimulating cAMP production of a mouse Sertoli cell line (MSC-1) expressing the recombinant rat FSH receptor. The circulating half-times (T1/2) of rat and human rec hormones were assessed after i.v. injections into adult male rats rendered gonadotropin-deficient by treatment with a gonadotropin-releasing hormone antagonist. A novel immunometric assay was used for the rat FSH measurements. In the one-component model the T1/2 values of rat and human recLH were 18.2 +/- 1.9 min (n = 7) and 44.6 +/- 3.1 min (n = 7) respectively and those of rat and human recFSH were 88.4 +/- 10.7 min (n = 6) and 55.0 +/- 4.2 min (n = 6) respectively; the two-component models revealed similar differences between the rec hormone preparations. Collectively, rat recLH was eliminated significantly faster from the circulation than human recLH (P < 0.0001). In contrast, the elimination of rat recFSH was significantly slower than that of human recFSH (P = 0.02). In conclusion, rat recFSH and rat recLH display lower biopotencies per unit mass than the respective human hormones in vitro, and also in vivo for LH. This is paralleled by shorter T1/2 of rat recLH than the respective human hormone in the circulation, whereas human recFSH has a shorter T1/2 than human FSH. The special functional features of the rat rec gonadotropins emphasize the use of these preparations on studies of gonadotropin function in the rat, an important animal model for reproductive physiology.

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