Novel presence of luteinizing hormone/human chorionic gonadotropin (hCG) receptors and the down-regulating action of hCG on gonadotropin- releasing hormone gene expression in immortalized hypothalamic GT1-7 neurons

Abstract. The aim of the present study was to examine the induction of ovulation during pregnancy, pseudopregnancy, and suckling-delayed pregnancy in mice using exogenous gonadotropins. The present results demonstrate that there are mature follicles in the ovary which can be induced to ovulate with administration of either exogenous human chorionic gonadotropin (hCG) or luteinizing hormone (LH) during pregnancy (Days 1–12) and pseudopregnancy (Days 4–8) in the mouse. hCG was relatively ineffective in initiating ovulation during suckling-delayed pregnancy, and hCG could not induce ovulation on Days 3–6 in any animals, suggesting that follicular growth is not continuous during suckling-delayed pregnancy in the mouse. Ovulation occurred in pregnant and pseudopregnant mice following injection of gonadotropin releasing hormone (GnRH) in a gelatin delay vehicle. Injection of GnRH in saline did not initiate ovulation in pregnant or pseudopregnant mice. A large release of LH was shown to occur following injection of GnRH in gelatin, but no release occurred after the same dose of GnRH in saline. In conclusion, the experiments demonstrate the existence of mature follicles during murine pregnancy and pseudopregnancy, and the lack of inductable follicles during suckling-delayed pregnancy.

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Gonadotropin-Releasing Hormone Agonist Versus Recombinant Human Chorionic Gonadotropin Triggering in Fertility Preservation Cycles

Reproductive Sciences ◽

10.1007/s43032-021-00622-2 ◽

2021 ◽

Author(s):

Einat Haikin Herzberger ◽

Sabaa Knaneh ◽

Hadar Amir ◽

Adi Reches ◽

Dalit Ben-Yosef ◽

...

Keyword(s):

Fertility Preservation ◽

Chorionic Gonadotropin ◽

Human Chorionic Gonadotropin ◽

Gonadotropin Releasing Hormone ◽

Releasing Hormone ◽

Gonadotropin Releasing Hormone Agonist

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Priming with a gonadotropin-releasing hormone agonist before immature oocyte retrieval may improve maturity of oocytes and outcome in in vitro maturation (IVM) cycle: a case report

Journal of Medical Case Reports ◽

10.1186/s13256-021-02706-8 ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

A. Smirnova ◽

M. Anshina ◽

E. Shalom Paz ◽

A. Ellenbogen

Keyword(s):

Fertility Preservation ◽

Chorionic Gonadotropin ◽

Human Chorionic Gonadotropin ◽

Oocyte Retrieval ◽

Fertilization Rate ◽

Gonadotropin Releasing Hormone ◽

Releasing Hormone ◽

Nuclear Maturation ◽

Gonadotropin Releasing Hormone Agonist

Abstract Background The concept of using a gonadotropin-releasing hormone agonist (GnRH-a) instead of human chorionic gonadotropin for triggering ovulation in patients treated with an antagonist protocol for in vitro fertilization (IVF) has become a routine clinical practice. It may promote oocyte nuclear maturation, resumption of meiosis and cumulus expansion. It seems that this attempt could be beneficial in an in vitro maturation (IVM) oocyte cycle performed for polycystic ovarian syndrome as well as for other indications such as urgent fertility preservation in patients with malignancies or unusual indications. Case presentation We present the case of a Caucasian patient who needed fertility preservation when routine natural IVF treatment did not yield oocyte retrieval, followed by three IVM cycles, priming ovulation with a GnRH-a. In total, 12 oocytes were obtained, all matured 4.5 hours after incubation in maturation media. The fertilization rate after intracytoplasmic sperm injection was 83%. Six good-quality embryos were vitrified. Conclusions It seems that triggering with a GnRH-a in selected cases may replace human chorionic gonadotropin in IVM of oocytes and could be highly beneficial in terms of obtaining high-grade embryos and possible pregnancy.

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