Germ Cell-Specific Expression and Biochemical Characterization of Mouse PAD6.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 297-297
Author(s):  
Keiichiro Yogo ◽  
Grisnarong Wongbandue ◽  
Akihito Ishigami ◽  
Hidenari Takahara ◽  
Tetsuya Kohsaka
Zygote ◽  
2006 ◽  
Vol 14 (3) ◽  
pp. 201-208 ◽  
Author(s):  
Hong Jin ◽  
Hiroshi Yoshitake ◽  
Hiroki Tsukamoto ◽  
Mai Takahashi ◽  
Miki Mori ◽  
...  

SummaryTEX101, a glycoprotein we recently identified, is primarily characterized as a unique germ-cell-specific marker protein that shows sexually dimorphic expression during mouse gonad development. Based on data obtained from molecular biological as well as immuno-morphological studies, we believe this molecule may play a role in the process underlying germ cell formation. However, many points remain unclear as the molecular characteristics and its physiological functions are far from being completely understood. To clarify the molecular basis of TEX101, we herein report a further biochemical characterization of the molecule using testicular Triton X-100 extracts from mice. Deglycosylation studies using endoglycohydrolases that delete N-linked oligosaccharides (OS) from the molecule show that TEX101 is highly (approximately 47%) N-glycosylated. All potential N-glycosylation sites within TEX101 are glycosylated and most of these sites are occupied by endoglycosidase F2-sensitive biantennary complex type OS units. In addition, an extremely low population among TEX101 possesses only endoglycosidase H-sensitive hybrid type OS units. In studies using phosphatidylinositol-specific phospholipase C against native testicular cells or TEX101 transfectant, the enzyme treatment caused major reduction of the TEX101 expression on the cell, suggesting that TEX101, at least in part, is expressed as a glycosylphosphatidylinositol-anchored protein. Taken together, these findings will help elucidate the molecular nature of TEX101, a marker molecule that appeared on germ cells during gametogenesis.


2005 ◽  
Vol 72 (3) ◽  
pp. 320-328 ◽  
Author(s):  
Gab Sang Lee ◽  
Hye Soo Kim ◽  
So Hyun Lee ◽  
Min Soo Kang ◽  
Dae Yong Kim ◽  
...  

2008 ◽  
Vol 6 (1) ◽  
pp. 32 ◽  
Author(s):  
Namhoe Baek ◽  
Jong-Min Woo ◽  
Cecil Han ◽  
Eunyoung Choi ◽  
Inju Park ◽  
...  

Development ◽  
1986 ◽  
Vol 93 (1) ◽  
pp. 197-211
Author(s):  
Kathleen B. Bechtol ◽  
Wai Chang HO ◽  
Steven Vaupel

The XT-1-molecule, an adhesion-related differentiation antigen of male mouse germ cells, is a 34000 Mr glycoprotein with major charge isomer at pI 5·1 and is an integral component of the cell membrane. On large late pachytene spermatocyte, the molecule is present at a concentration of 2·5×103 molecules µm−2, which approximates HLA/ABC concentration on lymphocytes. By comparing the reactivity of four anti-XT-1 monoclonal antibodies, three of which elicit germ cell-germ cell adhesion, we have defined two distinct surface regions of the XT-1-molecule. The relationship of the XT-1-molecule with other known adhesion-related molecules and testicular antigens is discussed.


Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 89-95 ◽  
Author(s):  
U.K. Ewulonu ◽  
T.J. Buratynski ◽  
J.C. Schimenti

Mouse t haplotypes contain several mutant alleles that disrupt spermatogenesis. Their phenotypes include sterility, reduced fertility and transmission ratio distortion (TRD). The substantial genetic analyses of these mutant alleles, coupled with intensive physical characterization of the t complex, provides a fertile ground for identifying and understanding genes essential to male gametogenesis. The t complex responder (Tcr) locus plays a central role in this process, interacting with other t haplotype-encoded genes to mediate TRD. A candidate responder gene, Tcp-10bt, has been cloned and subjected to molecular characterization. Here, we define the transcriptional regulatory regions of this gene in transgenic mice. A 1.6 kb (but not 0.6 kb) DNA fragment upstream of the transcription start site contains all the regulatory signals for appropriate temporal and germ cell-specific expression of this gene. Two smaller fragments within this region bound specifically to nuclear factor(s) from germ cell protein extracts in gel shift assays. This work is a step towards understanding the mechanism of Tcp-10bt regulated expression and may ultimately help reveal a common regulatory pathway shared by other similarly expressed spermatogenic genes.


2021 ◽  
Author(s):  
Tianlin Pei ◽  
Tian Li ◽  
Xiaoqiang Li ◽  
Yijia Yin ◽  
Mengying Cui ◽  
...  

Flavonoid glycosides extracted from roots of Scutellaria baicalensis exhibit strong pharmaceutical effect in antitumor, antioxidative, anti-inflammatory, and antiviral activity. UDP glycosyltransferase family members are responsible for the transfer of a glycosyl moiety from UDP sugars to a wide range of acceptor flavonoids. Here, we report the phylogenetic analysis, tissue-specific expression and biochemical characterization of 10 glucosyltrasferases (SbUGTs) and 6 glucuronosyltransferases (SbUGATs) based on the recently released genome of S. baicalensis. These results reveal that the high expression level and affinity to substrate of SbUGAT4 make baicalin become the richest flavonoid glycoside in the root of S. baicalensis.


Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.


2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.


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