A novel, tissue-specific integrin subunit, beta nu, expressed in the midgut of Drosophila melanogaster

Development ◽  
1993 ◽  
Vol 118 (3) ◽  
pp. 845-858 ◽  
Author(s):  
G.H. Yee ◽  
R.O. Hynes
Keyword(s):  
Drosophila Melanogaster ◽  
Matrix Proteins ◽  
Beta Subunits ◽  
Integrin Subunit ◽  
Integrin Receptor ◽  
Tissue Specific ◽  
Surface Receptors ◽  
Polymerase Chain ◽  
Maximal Expression

The integrins are a family of cell surface receptors for extracellular matrix proteins and counter-receptors on other cells. We have used the polymerase chain reaction to identify a novel integrin receptor beta subunit in Drosophila melanogaster. The deduced amino acid sequence of this subunit, which we have termed beta v (beta-neu), indicates that it has several unusual properties. The beta v subunit is roughly 33% identical with each of the previously sequenced vertebrate and Drosophila beta subunits and is lacking four of the 56 cysteine residues characteristic of most members of this protein family. The expression of the beta v gene is strikingly restricted. It is temporally regulated, with maximal expression occurring at 12–15 hours of embryonic development. In situ hybridization analyses and antibody localization on whole-mount embryos reveal that beta v expression is tissue-specific and confined to the developing midgut endoderm and its precursors during embryogenesis. Tissue specificity of expression is maintained through later stages of development as beta v transcripts are found exclusively in the larval midgut. Within this structure, beta v transcripts are especially concentrated in the cells of the midgut imaginal islands which give rise to the adult midgut.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

In Situ Polymerase Chain Reaction: Overview of Procedures and Applications

1998 ◽  
Vol 18 (3) ◽  
pp. 231-253 ◽  
Author(s):  
Carlos Muro-Cacho
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals The Alcohol Dehydrogenase Gene Is Nested in the outspread Locus of Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 143 (2) ◽  
pp. 897-911 ◽  
Author(s):  
S McNabb ◽  
S Greig ◽  
T Davis
Keyword(s):  
Drosophila Melanogaster ◽  
Alcohol Dehydrogenase ◽  
Pcr Amplification ◽  
Transcription Unit ◽  
Adjacent Gene ◽  
Dehydrogenase Gene ◽  
Chromosomal Breakpoints ◽  
Splicing Patterns ◽  
Somatic Musculature

Abstract This report describes the structure and expression of the outspread (osp) gene of Drosophila melanogaster. Previous work showed that chromosomal breakpoints associated with mutations of the osp locus map to both sides of the alcohol dehydrogenase gene (Adh), suggesting that Adh and the adjacent gene Adh' are nested in osp. We extended a chromosomal walk and mapped additional osp mutations to define the maximum molecular limit of osp as 119 kb. We identified a 6-kb transcript that hybridizes to osp region DNA and is altered or absent in osp mutants. Accumulation of this RNA peaks during embryonic and pupal periods. The osp cDNAs comprise two distinct classes based on alternative splicing patterns. The 5′ end of the longest cDNA was extended by PCR amplification. When hybridized to the osp walk, the 5′ extension verifies that Adh and Adh' are nested in osp and shows that osp has a transcription unit of ≥74 kb. In situ hybridization shows that osp is expressed both maternally and zygotically. In the ovary, osp is transcribed in nurse cells and localized in the oocyte. In embryos, expression is most abundant in the developing visceral and somatic musculature.

scholarly journals Cloning of the H,K-ATPase beta subunit. Tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase beta subunits.

1990 ◽  
Vol 265 (32) ◽  
pp. 19878-19884 ◽  
Author(s):  
V A Canfield ◽  
C T Okamoto ◽  
D Chow ◽  
J Dorfman ◽  
P Gros ◽  
...  
Keyword(s):  
Beta Subunit ◽  
Chromosomal Assignment ◽  
Beta Subunits ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Molecular Characterization of hobo-Mediated Inversions in Drosophila melanogaster

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 647-656
Author(s):  
William B Eggleston ◽  
Nac R Rim ◽  
Johng K Lim
Keyword(s):  
X Chromosome ◽  
Polymerase Chain Reaction Amplification ◽  
Polytene Chromosomes ◽  
Chromosomal Inversions ◽  
Hobo Element ◽  
Reaction Amplification ◽  
Notch Locus ◽  
Polymerase Chain

Abstract The structure of chromosomal inversions mediated by hobo transposable elements in the Uc-1 X chromosome was investigated using cytogenetic and molecular methods. Uc-1 contains a phenotypically silent hobo element inserted in an intron of the Notch locus. Cytological screening identified six independent Notch mutations resulting from chromosomal inversions with one breakpoint at cytological position 3C7, the location of Notch. In situ hybridization to salivary gland polytene chromosomes determined that both ends of each inversion contained hobo and Notch sequences. Southern blot analyses showed that both breakpoints in each inversion had hobo-Notch junction fragments indistinguishable in structure from those present in the Uc-1 X chromosome prior to the rearrangements. Polymerase chain reaction amplification of the 12 hobo-Notch junction fragments in the six inversions, followed by DNA sequence analysis, determined that each was identical to one of the two hobo-Notch junctions present in Uc-1. These results are consistent with a model in which hobo-mediated inversions result from homologous pairing and recombination between a pair of hobo elements in reverse orientation.

scholarly journals Comparison of culture, polymerase chain reaction, and fluorescent in situ hybridization for detection ofBrachyspira hyodysenteriaeand “Brachyspira hampsonii” in pig feces

2014 ◽  
Vol 27 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Bailey L. Wilberts ◽  
Hallie L. Warneke ◽  
Leslie P. Bower ◽  
Joann M. Kinyon ◽  
Eric R. Burrough
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of human papillomavirus infection in tissue blocks by in situ hybridization as compared with a polymerase chain reaction procedure

1991 ◽  
Vol 22 (6) ◽  
pp. 578-582 ◽  
Author(s):  
Barbro Skyldberg ◽  
Mina Kalantari ◽  
Marja Kärkl ◽  
Bo Johansson ◽  
Björn Hagmar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Procedure ◽  
Human Papillomavirus Infection ◽  
Papillomavirus Infection ◽  
Polymerase Chain

scholarly journals Qualitative and Quantitative Assessment of Modified In Situ Reverse Transcription-Polymerase Chain Reaction (In Situ RT-PCR) Method.

1999 ◽  
Vol 32 (5) ◽  
pp. 423-430 ◽  
Author(s):  
Jun Watanabe ◽  
Yuichi Sato ◽  
Benio Tsuchiya ◽  
Hiroyuki Kuramoto ◽  
Torn Kameya
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Quantitative Assessment ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Qualitative And Quantitative ◽  
Pcr Method ◽  
Polymerase Chain
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