Specification of bundle sheath cell fates during maize leaf development: roles of lineage and positional information evaluated through analysis of the tangled1 mutant

In leaves of the maize tangled1 (tan1) mutant, clusters of bundle sheath (BS)-like cells extend several cells distant from the veins, in association with the single layer of BS cells around the vein. We show that the BS-like cell clusters in tan1 leaves result from the continued division of cells in the procambial/BS cell lineage that do not divide further in wild-type leaves. The ectopic BS-like cells accumulate the BS marker NADP-dependent malic enzyme but not the mesophyll cell marker phosphoenolpyruvate carboxylase, and exhibit thickened walls, suggesting that they differentiate as C4-type BS cells. We propose that bundle sheath cell fate can be conferred on some derivatives of procambial cell divisions in a manner that is heritable through multiple cell divisions and is position-independent.

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SCARECROW, SCR-LIKE 23 and SHORT-ROOT control bundle sheath cell fate and function inArabidopsis thaliana

The Plant Journal ◽

10.1111/tpj.12470 ◽

2014 ◽

Vol 78 (2) ◽

pp. 319-327 ◽

Cited By ~ 45

Author(s):

Hongchang Cui ◽

Danyu Kong ◽

Xiuwen Liu ◽

Yueling Hao

Keyword(s):

Cell Fate ◽

Bundle Sheath ◽

Sheath Cell ◽

Short Root ◽

Bundle Sheath Cell ◽

And Function

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Identification of bundle sheath cell fate factors provides new tools for C3-to-C4 engineering

Plant Signaling & Behavior ◽

10.4161/psb.29162 ◽

2014 ◽

Vol 9 (6) ◽

pp. e29162 ◽

Cited By ~ 2

Author(s):

Xiaorong Gao ◽

Chaolun Wang ◽

Hongchang Cui

Keyword(s):

Cell Fate ◽

Bundle Sheath ◽

Sheath Cell ◽

Bundle Sheath Cell

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Four Mutant Alleles Elucidate the Role of the G2 Protein in the Development of C4 and C3 Photosynthesizing Maize Tissues

Genetics ◽

10.1093/genetics/159.2.787 ◽

2001 ◽

Vol 159 (2) ◽

pp. 787-797

Author(s):

Lizzie Cribb ◽

Lisa N Hall ◽

Jane A Langdale

Keyword(s):

Cell Types ◽

Bundle Sheath ◽

Primary Role ◽

Ribulose Bisphosphate Carboxylase ◽

Sheath Cell ◽

Mesophyll Cells ◽

Phenotypic Data ◽

Bisphosphate Carboxylase ◽

Bundle Sheath Cell

Abstract Maize leaf blades differentiate dimorphic photosynthetic cell types, the bundle sheath and mesophyll, between which the reactions of C4 photosynthesis are partitioned. Leaf-like organs of maize such as husk leaves, however, develop a C3 pattern of differentiation whereby ribulose bisphosphate carboxylase (RuBPCase) accumulates in all photosynthetic cell types. The Golden2 (G2) gene has previously been shown to play a role in bundle sheath cell differentiation in C4 leaf blades and to play a less well-defined role in C3 maize tissues. To further analyze G2 gene function in maize, four g2 mutations have been characterized. Three of these mutations were induced by the transposable element Spm. In g2-bsd1-m1 and g2-bsd1-s1, the element is inserted in the second intron and in g2-pg14 the element is inserted in the promoter. In the fourth case, g2-R, four amino acid changes and premature polyadenylation of the G2 transcript are observed. The phenotypes conditioned by these four mutations demonstrate that the primary role of G2 in C4 leaf blades is to promote bundle sheath cell chloroplast development. C4 photosynthetic enzymes can accumulate in both bundle sheath and mesophyll cells in the absence of G2. In C3 tissue, however, G2 influences both chloroplast differentiation and photosynthetic enzyme accumulation patterns. On the basis of the phenotypic data obtained, a model that postulates how G2 acts to facilitate C4 and C3 patterns of tissue development is proposed.

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Successive specification of Drosophila neuroblasts NB 6-4 and NB 7-3 depends on interaction of the segment polarity genes wingless, gooseberry and naked cuticle

Development ◽

10.1242/dev.128.17.3253 ◽

2001 ◽

Vol 128 (17) ◽

pp. 3253-3261 ◽

Cited By ~ 1

Author(s):

Nirupama Deshpande ◽

Rainer Dittrich ◽

Gerhard M. Technau ◽

Joachim Urban

Keyword(s):

Cell Fate ◽

Cell Lineage ◽

Positional Information ◽

Dorsoventral Patterning ◽

Expression Domain ◽

Direct Role ◽

Segment Polarity Genes ◽

Segment Polarity ◽

Unique Identity

The Drosophila central nervous system derives from neural precursor cells, the neuroblasts (NBs), which are born from the neuroectoderm by the process of delamination. Each NB has a unique identity, which is revealed by the production of a characteristic cell lineage and a specific set of molecular markers it expresses. These NBs delaminate at different but reproducible time points during neurogenesis (S1-S5) and it has been shown for early delaminating NBs (S1/S2) that their identities depend on positional information conferred by segment polarity genes and dorsoventral patterning genes. We have studied mechanisms leading to the fate specification of a set of late delaminating neuroblasts, NB 6-4 and NB 7-3, both of which arise from the engrailed (en) expression domain, with NB 6-4 delaminating first. In contrast to former reports, we did not find any evidence for a direct role of hedgehog in the process of NB 7-3 specification. Instead, we present evidence to show that the interplay of the segmentation genes naked cuticle (nkd) and gooseberry (gsb), both of which are targets of wingless (wg) activity, leads to differential commitment to NB 6-4 and NB 7-3 cell fate. In the absence of either nkd or gsb, one NB fate is replaced by the other. However, the temporal sequence of delamination is maintained, suggesting that formation and specification of these two NBs are under independent control.

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