Closer vein spacing by ectopic expression of nucleotide-binding and leucine-rich repeat proteins in rice leaves

Key message Elevated expression of nucleotide-binding and leucine-rich repeat proteins led to closer vein spacing and higher vein density in rice leaves. Abstract To feed the growing global population and mitigate the negative effects of climate change, there is a need to improve the photosynthetic capacity and efficiency of major crops such as rice to enhance grain yield potential. Alterations in internal leaf morphology and cellular architecture are needed to underpin some of these improvements. One of the targets is to generate a “Kranz-like” anatomy in leaves that includes decreased interveinal spacing close to that in C4 plant species. As C4 photosynthesis has evolved from C3 photosynthesis independently in multiple lineages, the genes required to facilitate C4 may already be present in the rice genome. The Taiwan Rice Insertional Mutants (TRIM) population offers the advantage of gain-of-function phenotype trapping, which accelerates the identification of rice gene function. In the present study, we screened the TRIM population to determine the extent to which genetic plasticity can alter vein density (VD) in rice. Close vein spacing mutant 1 (CVS1), identified from a VD screening of approximately 17,000 TRIM lines, conferred heritable high leaf VD. Increased vein number in CVS1 was confirmed to be associated with activated expression of two nucleotide-binding and leucine-rich repeat (NB-LRR) proteins. Overexpression of the two NB-LRR genes individually in rice recapitulates the high VD phenotype, due mainly to reduced interveinal mesophyll cell (M cell) number, length, bulliform cell size and thus interveinal distance. Our studies demonstrate that the trait of high VD in rice can be achieved by elevated expression of NB-LRR proteins limited to no yield penalty.

Supplemental Material: Age, material source, and formation mechanism of bedding-parallel calcite beef veins: Case from the mature Eocene lacustrineshales in the Biyang Sag, Nanxiang Basin, China

Figure S1: Correlations of vein density vs. TOC, calcite, and dolomite contents of host shales; Figure S2: The box plots of calcite contents in shale samples with and without beef veins; Table S1: LA-MC-ICP-MS carbonate U-Pb dating data.

Supplemental Material: Age, material source, and formation mechanism of bedding-parallel calcite beef veins: Case from the mature Eocene lacustrineshales in the Biyang Sag, Nanxiang Basin, China

Figure S1: Correlations of vein density vs. TOC, calcite, and dolomite contents of host shales; Figure S2: The box plots of calcite contents in shale samples with and without beef veins; Table S1: LA-MC-ICP-MS carbonate U-Pb dating data.

Vein topology, structures, and distribution during the prograde formation of an Archean gold stockwork

The Archean Cheechoo stockwork gold deposit is hosted by a felsic intrusion of tonalitic-granodioritic composition and crosscutting pegmatite dikes in the Eeyou Istchee James Bay area of Quebec, Canada (Archean Superior craton). The evolution of the stockwork is characterized herein using field relationships, vein density, and connectivity measurements on drill core and outcrop zones. The statistical distribution of gold is used to highlight mechanisms of stockwork emplacement and gold mineralization and remobilization. Two statistical populations of gold concentration are present. Population A is represented by gold grades below 1 g/t with a lognormal cumulative frequency. It is widespread in the hydrothermally altered (albite and quartz) and mineralized facies of the pluton. It is controlled by the development of quartz-feldspar-diopside veins as shown by the similar lognormal distribution of grades and vein density and by the correspondence of grades with network connectivity. Diopside and actinolite porphyroblasts in deformed veins within sodic and calcsilicate alteration zones are evidence for auriferous vein emplacement prior to the amphibolite facies peak of metamorphism. Population B (>1 g/t) is erratic and exhibits a strong nugget effect. It is present throughout the mineralized portion of the pluton and in pegmatites. This population is interpreted as the result of gold remobilization during prograde metamorphism and pegmatite emplacement following the metamorphic peak. The pegmatites are interpreted to have scavenged gold emplaced prior to peak metamorphism. These results show the isotropic behavior of the investigated stockwork during regional deformation and its development during the early stages of regional prograde metamorphism.

Analysis of Classic Tomato Mutants Reveals Influence of Leaf Vein Density on Fruit BRIX

Tomato bipinnate (bip) is a classic leaf mutant, with highly increased leaf complexity resulting from the loss of function of a BEL-LIKE HOMEODAMAIN (BELL) gene. Here, we analyzed several bip mutants and their isogenic wildtype backgrounds for a suite of leaf morphology traits, ranging from leaf complexity, leaflet shape and size, to leaf vascular density to investigate how changes in leaf morphology influence fruit traits. Our analyses showed an unexpected relationship between leaf vein density and fruit sugar levels, where leaf vein density was negatively correlated with fruit BRIX. RNA-Seq analysis suggested variation in Glucose-6-phosphate translocator2 (GPT2) gene expression caused correlated changes in leaf vein density and BRIX when bip mutant and wildtype were compared, suggesting that the correlation between leaf vein density and fruit sugar may result from the genes regulating leaf vein development that are also involved in regulating leaf sugar biosynthesis. Our results provide a resource for further exploration of the genetic basis for the complex relationship between fruit quality and leaf traits in natural populations.

Leaf Vein Density of Tree Saplings Composing Lower Canopy in Tropical Forest Reflects Their Ecophysiological Characteristics

One factor affecting the survival of a species in a tropical ecosystem is its ability to respond to environmental conditions, which depend on their ecophysiological performances. Plants ability to transport water as a major environmental factor would determine their survival. The anatomy of xylem inside leaves and stem as water conductive tissue will dictate the rate of water transport through the plant stem and leaves. Leaf vein, which contains xylem vessels, dictates water transport through leaves and plant’s ability to control water loss through stomata. This research found that tree saplings composing a lower canopy of tropical forests have different ecophysiological attributes. Pioneer species, such as Cinnamomum sp., Diospyros macrophylla, Castanopsis costata, Elateriospermum tapos, and Ziziphus sp., have higher leaf vein density than primary species, such as a member of genus Garcinia, Shorea, Dipterocarpus, and Syzigium. It implies that pioneer species might have higher rates of water transport and consequently, higher rates of photosynthesis. If forest vegetation was more opened, then pioneer species may dominate the area as they are more tolerant of light. The Composition of forest vegetation with different ecophysiological characteristics may affect the forest dynamics and hydrological cycle.

Leaf and Stem Traits are Linked to Liana Growth Rate in a Subtropical Cloud Forest

There is accumulating evidence that the abundance and biomass of lianas are increasing with global climate change in the Neotropics. However, our knowledge of growth–trait relationships among lianas is surprisingly rare. Here, we monitored the relative growth rate of 2860 individuals from seven deciduous and four evergreen liana species in a 20 ha subtropical cloud forest dynamics plot at high elevation (2472–2628 m a.s.l.) in southwest China. We linked the relative growth rate of lianas with nine leaf traits associated with leaf morphology, nutrient concentrations, and water hydraulic capacity as indicated by leaf vein density, and five stem wood traits related to stem water transport capacity and wood density. Our results showed that deciduous lianas have higher relative growth rates than their evergreen counterparts. Across all lianas studied, the relative growth rate was positively correlated with the leaf area and specific leaf area, but negatively correlated with leaf dry matter content. The relative growth rate of lianas was strongly correlated with nitrogen concentration after excluding the legume liana species. The relative growth rate was decoupled from leaf phosphorus and potassium concentrations, leaf vein density, and stem vessel traits across all lianas investigated. For four evergreen lianas, there were positive associations of the relative growth rate with the leaf thickness and diameter of the largest vessels. This study is the first to illustrate the relationships of liana growth with leaf and stem traits in the high-elevation subtropical cloud forest. More studies from diverse forest ecosystems are needed to comprehensively understand the mechanism underlying liana growth patterns.

C4 photosynthesis with Kranz anatomy evolved in the Oryza coarctata Roxb

The C4 cycle is a complex biochemical pathway that has been evolved in plants to deal with the adverse environmental conditions. Mostly C4 plants grow in arid, water-logged area or poor nutrient habitats. Wild species, Oryza coarctata (genome type KKLL; chromosome number (4x) =48, genome size 665 Mb) belongs to the genus of Oryza which thrives well under high saline as well as submerged conditions. Here, we report for the first time that O. coarctata is a C4 plant by observing the increased biomass growth, morphological features such as vein density, anatomical features including ultrastuctural characteristics as well as expression patterns of C4 related genes. Leaves of O. coarctata have higher vein density and possess Kranz anatomy. The ultrastructural observation showed chloroplast dimorphism i.e. presence of agranal chloroplasts in bundle sheath cells whereas, mesophyll cells contain granal chloroplasts. The cell walls of bundle sheath cells contain tangential suberin lamella. The transcript level of C4 specific genes such as phosphoenolpyruvate carboxylase, pyruvate orthophosphate dikinase, NADP-dependent malic enzyme and malate dehydrogenase was higher in leaves of O. coarctata compare to high yielding rice cultivar (IR-29). These anatomical, ultra structural as well as molecular changes in O. coarctata for C4 photosynthesis adaptation might be might be due to its survival in wide diverse condition from aquatic to saline submerged condition. Being in the genus of Oryza, this plant could be potential donor for production of C4 rice in future through conventional breeding, as successful cross with rice has already been reported.