A wide reprogramming of histone H3 modifications during male meiosis I in rice is dependent on the Argonaute protein MEL

Spindle formation and chromosome elimination during male meiosis in Sciara ocellaris (Diptera, Sciaridae) has been studied by immunofluorescence techniques. During meiosis I a monopolar spindle is formed from a single polar complex (centrosome-like structure). This single centrosomal structure persists during meiosis II and is responsible for the non-disjunction of the maternal X chromatids. During meiosis I and II non-spindle microtubules are assembled in the cytoplasmic bud regions of the spermatocytes. The chromosomes undergoing elimination during both meiotic divisions are segregated to the bud region where they associate with bundles of microtubules. The presence and distribution of centrosomal antigens in S. ocellaris meiotic spindles and bud regions has been investigated using different antibodies. gamma-Tubulin and centrin are present in the bud as well as in the single polar complex of first meiotic spindle. The results suggest that spermatocyte bud regions contain microtubule-organizing centres (MTOCs) that nucleate cytoplasmic microtubules that are involved in capturing chromosomes in the bud regions. The distribution of actin and myosin in the spermatocytes during meiosis is also reported.

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Homolog pairing and sister chromatid cohesion in heterochromatin in Drosophila male meiosis I

Chromosoma ◽

10.1007/s00412-011-0314-0 ◽

2011 ◽

Vol 120 (4) ◽

pp. 335-351 ◽

Cited By ~ 14

Author(s):

Jui-He Tsai ◽

Rihui Yan ◽

Bruce D. McKee

Keyword(s):

Sister Chromatid ◽

Sister Chromatid Cohesion ◽

Male Meiosis ◽

Homolog Pairing ◽

Chromatid Cohesion ◽

Meiosis I

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Histone H3 phosphorylation and non-disjunction of the maternal X chromosome during male meiosis in sciarid flies

Journal of Cell Science ◽

10.1242/jcs.083022 ◽

2011 ◽

Vol 124 (10) ◽

pp. 1715-1725 ◽

Cited By ~ 8

Author(s):

M. C. Escriba ◽

M. C. Giardini ◽

C. Goday

Keyword(s):

X Chromosome ◽

Histone H3 ◽

Male Meiosis ◽

H3 Phosphorylation ◽

Histone H3 Phosphorylation

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Aurora-C Kinase Deficiency Causes Cytokinesis Failure in Meiosis I and Production of Large Polyploid Oocytes in Mice

Molecular Biology of the Cell ◽

10.1091/mbc.e10-02-0170 ◽

2010 ◽

Vol 21 (14) ◽

pp. 2371-2383 ◽

Cited By ~ 88

Author(s):

Kuo-Tai Yang ◽

Shu-Kuei Li ◽

Chih-Chieh Chang ◽

Chieh-Ju C. Tang ◽

Yi-Nan Lin ◽

...

Keyword(s):

Chromosome Segregation ◽

Female Mouse ◽

Histone H3 ◽

Aurora B ◽

Binding Motif ◽

Mouse Oocytes ◽

Microtubule Attachment ◽

H3 Phosphorylation ◽

Histone H3 Phosphorylation ◽

Meiosis I

We previously isolated Aurora-C/Aie1 in a screen for kinases expressed in mouse sperm and eggs. Here, we show the localization of endogenous Aurora-C and examine its roles during female mouse meiosis. Aurora-C was detected at the centromeres and along the chromosome arms in prometaphase I–metaphase I and was concentrated at centromeres at metaphase II, in which Aurora-C also was phosphorylated at Thr171. During the anaphase I–telophase I transition, Aurora-C was dephosphorylated and relocalized to the midzone and midbody. Microinjection of the kinase-deficient Aurora-C (AurC-KD) mRNA into mouse oocytes significantly inhibited Aurora-C activity and caused multiple defects, including chromosome misalignment, abnormal kinetochore–microtubule attachment, premature chromosome segregation, and cytokinesis failure in meiosis I. Furthermore, AurC-KD reduced Aurora-C and histone H3 phosphorylation and inhibited kinetochore localization of Bub1 and BubR1. Similar effects also were observed in the oocytes injected with INCNEP-delIN mRNAs, in which the Aurora-C binding motif was removed. The most dramatic effect observed in AurC-KD–injected oocytes is cytokinesis failure in meiosis I, resulting in producing large polyploid oocytes, a pattern similar to Aurora-C deficiency human spermatozoa. Surprisingly, we detected no Aurora-B protein in mouse oocytes. We propose that Aurora-C, but not Aurora-B, plays essential roles in female mouse meiosis.

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Chromosome separation during Drosophila male meiosis I requires separase-mediated cleavage of the homolog conjunction protein UNO

PLoS Genetics ◽

10.1371/journal.pgen.1008928 ◽

2020 ◽

Vol 16 (10) ◽

pp. e1008928

Author(s):

Joe Weber ◽

Zeynep Kabakci ◽

Soumya Chaurasia ◽

Erich Brunner ◽

Christian F. Lehner

Keyword(s):

Male Meiosis ◽

Chromosome Separation ◽

Meiosis I

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An uncommon H3/Ser10 phosphorylation pattern in Cestrum strigilatum (Solanaceae), a species with B chromosomes

Genome ◽

10.1139/g08-042 ◽

2008 ◽

Vol 51 (9) ◽

pp. 772-777 ◽

Cited By ~ 5

Author(s):

Thiago Fernandes ◽

Priscila Mary Yuyama ◽

Ana Paula Moraes ◽

André Luís Laforga Vanzela

Keyword(s):

Histone H3 ◽

Pericentromeric Region ◽

B Chromosomes ◽

South American ◽

Terminal Portion ◽

Mendelian Segregation ◽

Cell Divisions ◽

Phosphorylation Pattern ◽

Mitosis And Meiosis ◽

Meiosis I

Cestrum strigilatum (Solanaceae) is a South American shrub with B chromosomes. Bs show a univalent behavior when a single B is present, have non-Mendelian segregation, and are poor in genes and rich in repetitive DNA. In this study, the histone H3 at serine 10 (H3/Ser10) phosphorylation pattern was investigated during mitosis and meiosis of C. strigilatum collected from the wild and was compared in A and B chromosomes. The results revealed that H3/Ser10 phosphorylation of A chromosomes occurred only in the pericentromeric region in both mitosis and meiosis, whereas in the B univalent, phosphorylation appeared in almost the whole extent of the chromosome, except in the terminal portion of the long arm. In meiosis II, the phosphorylation of A chromosomes was similar to that in the first division of meiosis, but the Bs did not show H3/Ser10 phosphorylation. Our results suggest that phosphorylation at the pericentromeric region may be associated with chromosome motility during cell divisions and with the cohesion of B chromatids in a univalent structure in meiosis I.

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