scholarly journals The mevalonate pathway is a crucial regulator of tendon cell specification

Development ◽  
2020 ◽  
Vol 147 (12) ◽  
pp. dev185389 ◽  
Author(s):  
Jessica W. Chen ◽  
Xubo Niu ◽  
Matthew J. King ◽  
Marie-Therese Noedl ◽  
Clifford J. Tabin ◽  
...  
Keyword(s):  
Mevalonate Pathway ◽  
Cell Specification ◽  
Tendon Cell

A Systems Approach to ER Stress Pathway Dynamics Reveals the Impact of Freeze-Dried Broccoli Extract to Mitigate Inflammation in Human Adipocytes through the Mevalonate Pathway

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2076-P
Author(s):  
ALICE MURPHY ◽  
SAHAR AZHARIAN ◽  
GYANENDRA TRIPATHI ◽  
GUY BARKER ◽  
MICHAEL J. CHAPPELL ◽  
...  
Keyword(s):  
Er Stress ◽  
Systems Approach ◽  
Mevalonate Pathway ◽  
Human Adipocytes ◽  
Freeze Dried ◽  
The Impact ◽  
Stress Pathway

Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome

2019 ◽  
Author(s):  
Patrick R. A. Zanon ◽  
Lisa Lewald ◽  
Stephan M. Hacker
Keyword(s):  
Binding Sites ◽  
Bacterial Resistance ◽  
Mevalonate Pathway ◽  
Rapid Development ◽  
High Reactivity ◽  
Functional Importance ◽  
Essential Proteins ◽  
Covalent Inhibitors ◽  
Druggable Targets ◽  
Covalent Ligands

Rapid development of bacterial resistance has led to an urgent need to find new druggable targets for antibiotics. In this context, residue-specific chemoproteomic approaches enable proteome-wide identification of binding sites for covalent inhibitors. Here, we describe isotopically labeled desthiobiotin azide (isoDTB) tags that are easily synthesized, shorten the chemoproteomic workflow and allow an increased coverage of cysteines in bacterial systems. We quantify 59% of all cysteines in essential proteins in <i>Staphylococcus aureus</i> and discover 88 cysteines with high reactivity, which correlates with functional importance. Furthermore, we identify 268 cysteines that are engaged by covalent ligands. We verify inhibition of HMG-CoA synthase, which will allow addressing the bacterial mevalonate pathway through a new target. Overall, a comprehensive map of the bacterial cysteinome is obtained, which will facilitate the development of antibiotics with novel modes-of-action.

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