scholarly journals MSL3 promotes germline stem cell differentiation in female Drosophila

Development ◽  
2021 ◽  
Author(s):  
Alicia McCarthy ◽  
Kahini Sarkar ◽  
Elliot T. Martin ◽  
Maitreyi Upadhyay ◽  
Seoyeon Jang ◽  
...  
Keyword(s):  
Rna Binding ◽  
Stem Cell Differentiation ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Cell Cycle Entry ◽  
Male Specific Lethal ◽  
Key Factor ◽  
Male Specific Lethal Complex ◽  
Ribosomal Protein S19 ◽  
Male Specific

Gamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation are incompletely understood. Set2, which deposits H3K36me3 modifications, is required for GSC differentiation during Drosophila oogenesis. We discovered that the H3K36me3 reader Male-specific lethal 3 (MSL3) and histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate GSC differentiation in female Drosophila. MSL3, acting independent from the rest of the male specific lethal complex, promotes transcription of genes including a germline enriched ribosomal protein S19 paralog, RpS19b. RpS19b upregulation is required for translation of RNA-binding Fox protein 1 (Rbfox1), a known meiotic cell cycle entry factor. Thus, MSL3 regulates GSC differentiation by modulating translation of a key factor that promotes transition to an oocyte fate.

scholarly journals MSL3 coordinates a transcriptional and translational meiotic program in female Drosophila

2019 ◽  
Author(s):  
Alicia McCarthy ◽  
Kahini Sarkar ◽  
Elliot T Martin ◽  
Maitreyi Upadhyay ◽  
Joshua R James ◽  
...  
Keyword(s):  
Histone Acetyltransferase ◽  
Germline Stem Cells ◽  
Meiotic Cell ◽  
Cell Cycle Entry ◽  
Gamete Formation ◽  
Male Specific Lethal ◽  
Genes Encoding ◽  
Ribosomal Protein S19 ◽  
Male Specific ◽  
Female Germline

SummaryGamete formation from germline stem cells (GSCs) is essential for sexual reproduction. However, the regulation of GSC differentiation and meiotic entry are incompletely understood. Set2, which deposits H3K36me3 modifications, is required for differentiation of GSCs during Drosophila oogenesis. We discovered that the H3K36me3 reader Male-specific lethal 3 (MSL3) and the histone acetyltransferase complex Ada2a-containing (ATAC) cooperate with Set2 to regulate entry into meiosis in female Drosophila. MSL3 expression is restricted to the mitotic and early meiotic stages of the female germline, where it promotes transcription of genes encoding synaptonemal complex components and a germline enriched ribosomal protein S19 paralog, RpS19b. RpS19b upregulation is required for translation of Rbfox1, a known meiotic cell cycle entry factor. Thus, MSL3 is a master regulator of meiosis, coordinating the expression of factors required for recombination and GSC differentiation. We find that MSL3 is expressed during mouse spermatogenesis, suggesting a conserved function during meiosis.

scholarly journals Structural insight into the regulation of MOF in the male-specific lethal complex and the non-specific lethal complex

Cell Research ◽  
2012 ◽  
Vol 22 (6) ◽  
pp. 1078-1081 ◽  
Author(s):  
Jing Huang ◽  
Bingbing Wan ◽  
Lipeng Wu ◽  
Yuting Yang ◽  
Yali Dou ◽  
...  
Keyword(s):  
Male Specific Lethal ◽  
Male Specific Lethal Complex ◽  
Structural Insight ◽  
Male Specific ◽  
Insight Into

Nanos and Pumilio have critical roles in the development and function of Drosophila germline stem cells

Development ◽  
1998 ◽  
Vol 125 (4) ◽  
pp. 679-690 ◽  
Author(s):  
A. Forbes ◽  
R. Lehmann
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Germ Cells ◽  
Rna Binding ◽  
Drosophila Embryo ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Embryonic Patterning ◽  
Germline Development ◽  
Egg Chambers

The zinc-finger protein Nanos and the RNA-binding protein Pumilio act together to repress the translation of maternal hunchback RNA in the posterior of the Drosophila embryo, thereby allowing abdomen formation. nanos RNA is localized to the posterior pole during oogenesis and the posteriorly synthesized Nanos protein is sequestered into the germ cells as they form in the embryo. This maternally provided Nanos protein is present in germ cells throughout embryogenesis. Here we show that maternally deposited Nanos protein is essential for germ cell migration. Lack of zygotic activity of nanos and pumilio has a dramatic effect on germline development of homozygous females. Given the coordinate function of nanos and pumilio in embryonic patterning, we analyzed the role of these genes in oogenesis. We find that both genes act in the germline. Although the nanos and pumilio ovarian phenotypes have similarities and both genes ultimately affect germline stem cell development, the focus of these phenotypes appears to be different. While pumilio mutant ovaries fail to maintain stem cells and all germline cells differentiate into egg chambers, the focus of nanos function seems to lie in the differentiation of the stem cell progeny, the cystoblast. Consistent with the model that nanos and pumilio have different phenotypic foci during oogenesis, we detect high levels of Pumilio protein in the germline stem cells and high levels of Nanos in the dividing cystoblasts. We therefore suggest that, in contrast to embryonic patterning, Nanos and Pumilio may interact with different partners in the germline.

scholarly journals Male-specific lethal complex inDrosophilacounteracts histone acetylation and does not mediate dosage compensation

2013 ◽  
Vol 110 (9) ◽  
pp. E808-E817 ◽  
Author(s):  
Lin Sun ◽  
Harvey R. Fernandez ◽  
Ryan C. Donohue ◽  
Jilong Li ◽  
Jianlin Cheng ◽  
...  
Keyword(s):  
Histone Acetylation ◽  
Dosage Compensation ◽  
Male Specific Lethal ◽  
Male Specific Lethal Complex ◽  
Male Specific
Sign in / Sign up

Export Citation Format

Share Document