The Cultivation of the Pituitary of Infantile Rats by the Glass-Rod Technique and the Influence of Grafted Explants on the Growth of Hypophysectomized Hosts
Just before the last war we made our first attempts to cultivate the anterior pituitary of rats and mice (Martinovitch, 1940). In those preliminary experiments we used pituitaries of animals 1 to 6 months old, and the culture medium was composed of chicken plasma and chicken embryo extract in equal proportions. The explants were grown by the watchglass technique and the incubating temperature was 33° to 34° C. Under these conditions some of our cultures survived for several months. Two months old cultures were grafted in the anterior eye chamber of normal animals and some of these grafts were successfully established. These initial results, and those obtained by Gaillard (1937,1942), seemed sufficiently promising to justify further research. Earlier work in vitro on the pituitary gland, e.g. the papers of Kasahara (1936), Anderson & Haymaker (1937), and Cutting & Lewis (1938), dealt mainly with unorganized growth.