Presence of a nuclear lamina in pachytene spermatocytes of the rat

1993 ◽  
Vol 104 (2) ◽  
pp. 557-563 ◽  
Author(s):  
B. Vester ◽  
A. Smith ◽  
G. Krohne ◽  
R. Benavente
Keyword(s):  
Nuclear Lamina ◽  
Cell Nuclei ◽  
Specific Expression ◽  
Intermediate Filament Proteins ◽  
Lamin B1 ◽  
Evolutionarily Conserved ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Specific Expression Pattern ◽  
Pachytene Spermatocytes

The nuclear lamina is a karyoskeletal structure located at the periphery of cell nuclei. The major constituents are the lamins, which belong to the evolutionarily conserved multigene family of intermediate filament proteins. Lamins show a conspicuous cell type-specific expression pattern. The majority of somatic cells of vertebrates express A-type (lamins A and C) as well as B-type (lamins B1 and B2) lamins. Although a lamina structure has been demonstrated to be a ubiquitous component of somatic nuclei its existence in certain meiotic stages during spermatogenesis has been a matter of debate. In this study, we investigated the expression of lamins in rat spermatogenic cells using immunological and protein-chemical methods. We report on the presence of a nuclear lamina structure in rat pachytene spermatocytes. With the aid of a novel broad-reacting lamin antibody we have demonstrated the expression of a protein that is closely related, if not identical, to lamin B1.

scholarly journals Cell-Type-Specific Expression Pattern of Proton-Sensing Receptors and Channels in Pituitary Gland

2020 ◽  
Vol 119 (11) ◽  
pp. 2335-2348
Author(s):  
Kai Wang ◽  
Karla Kretschmannova ◽  
Rafael M. Prévide ◽  
Kosara Smiljanic ◽  
Qing Chen ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Expression Pattern ◽  
Cell Type ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Specific Expression Pattern

Structural Studies on Different Ligand Binding Ability of Sialoadhesin Using Molecular Modeling Techniques

2020 ◽  
Vol 5 (4) ◽  
pp. 277-282
Author(s):  
Madhumita Dandopath Patra
Keyword(s):  
Molecular Modeling ◽  
Stable Complex ◽  
Free Energies ◽  
Specific Expression ◽  
Functional Roles ◽  
Modeling Techniques ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Specific Expression Pattern ◽  
Inflammatory Macrophages

Siglecs are the major homologous subfamily of I-type lectins with an ability to recognize sialylated glycans. Siglecs are attractive therapeutic targets because of their endocytic properties, ability to modulate receptor signaling and cell-type specific expression pattern. Sialoadhesin (Sn/ Siglec-1/ CD169), a member of the Siglec family expressed on subsets of resident and inflammatory macrophages and involves in modulation of inflammation and immunity. In this work, 3-D structure of human Siglec-1 (hSiglec-1) was predicted based on X-ray crystallo-graphically determined structure of mouse Siglec-1[mSiglec-1(PDB ID: 1QFP)] using molecular modeling techniques. The structure of complexes in solution of hSiglec-1 with ligands, glycopeptide and 3′-sialyllactose were predicted using a novel docking technique comprising of repeated cycles of molecular dynamics and energy minimization. Calculation of the free energies of binding of complexes suggested that glycopeptide can form stable complex with dissociation constant value of 3.31 μM whereas complex formation of 3′-sialyllactose with the protein in aqueous medium is thermodynamically unfavorable. The structural analysis of theses complexes represent the functional recognition interactions of this protein with the bound sugar molecule and as such provide detailed information about functional roles of such sugar binding protein.

Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis

Cell ◽  
1985 ◽  
Vol 41 (1) ◽  
pp. 177-190 ◽  
Author(s):  
Ricardo Benavente ◽  
Georg Krohne ◽  
Werner W. Franke
Keyword(s):  
Xenopus Laevis ◽  
Nuclear Lamina ◽  
Cell Type ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Cell Type Specific

Cell-type-specific expression pattern of ceramide synthase 2 protein in mouse tissues

2013 ◽  
Vol 140 (5) ◽  
pp. 533-547 ◽  
Author(s):  
Christiane Kremser ◽  
Anna-Lena Klemm ◽  
Martina Uelft ◽  
Silke Imgrund ◽  
Christina Ginkel ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Ceramide Synthase ◽  
Cell Type ◽  
Specific Expression ◽  
Mouse Tissues ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Specific Expression Pattern

scholarly journals A cell type‐specific expression map of NCoR1 and SMRT transcriptional co‐repressors in the mouse brain

2020 ◽  
Vol 528 (13) ◽  
pp. 2218-2238 ◽  
Author(s):  
Attilio Iemolo ◽  
Patricia Montilla‐Perez ◽  
I‐Chi Lai ◽  
Yinuo Meng ◽  
Syreeta Nolan ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Cell Type ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
A Cell ◽  
Cell Type Specific

scholarly journals Olfactory expression of trace amine-associated receptors requires cooperative cis-acting enhancers

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ami Shah ◽  
Madison Ratkowski ◽  
Alessandro Rosa ◽  
Paul Feinstein ◽  
Thomas Bozza
Keyword(s):  
Gene Expression ◽  
Large Family ◽  
Sequence Motifs ◽  
Specific Expression ◽  
Cis Acting ◽  
Conserved Sequence ◽  
Trace Amine ◽  
Sequence Elements ◽  
Cell Type Specific Expression ◽  
Cell Type Specific

AbstractOlfactory sensory neurons express a large family of odorant receptors (ORs) and a small family of trace amine-associated receptors (TAARs). While both families are subject to so-called singular expression (expression of one allele of one gene), the mechanisms underlying TAAR gene choice remain obscure. Here, we report the identification of two conserved sequence elements in the mouse TAAR cluster (T-elements) that are required for TAAR gene expression. We observed that cell-type-specific expression of a TAAR-derived transgene required either T-element. Moreover, deleting either element reduced or abolished expression of a subset of TAAR genes, while deleting both elements abolished olfactory expression of all TAARs in cis with the mutation. The T-elements exhibit several features of known OR enhancers but also contain highly conserved, unique sequence motifs. Our data demonstrate that TAAR gene expression requires two cooperative cis-acting enhancers and suggest that ORs and TAARs share similar mechanisms of singular expression.

scholarly journals Function of Circular RNAs in Fish and Their Potential Application as Biomarkers

2021 ◽  
Vol 22 (13) ◽  
pp. 7119
Author(s):  
Golam Rbbani ◽  
Artem Nedoluzhko ◽  
Jorge Galindo-Villegas ◽  
Jorge M. O. Fernandes
Keyword(s):  
Growth Regulation ◽  
Functional Characterization ◽  
Circular Rnas ◽  
Farmed Fish ◽  
Developmentally Regulated ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Taxonomic Groups ◽  
And Function

Circular RNAs (circRNAs) are an emerging class of regulatory RNAs with a covalently closed-loop structure formed during pre-mRNA splicing. Recent advances in high-throughput RNA sequencing and circRNA-specific computational tools have driven the development of novel approaches to their identification and functional characterization. CircRNAs are stable, developmentally regulated, and show tissue- and cell-type-specific expression across different taxonomic groups. They play a crucial role in regulating various biological processes at post-transcriptional and translational levels. However, the involvement of circRNAs in fish immunity has only recently been recognized. There is also broad evidence in mammals that the timely expression of circRNAs in muscle plays an essential role in growth regulation but our understanding of their expression and function in teleosts is still very limited. Here, we discuss the available knowledge about circRNAs and their role in growth and immunity in vertebrates from a comparative perspective, with emphasis on cultured teleost fish. We expect that the interest in teleost circRNAs will increase substantially soon, and we propose that they may be used as biomarkers for selective breeding of farmed fish, thus contributing to the sustainability of the aquaculture sector.

Sign in / Sign up

Export Citation Format

Share Document