Qualitative and Quantitative Analysis of Tumour Invasion In Vivo and In Vitro

1987 ◽  
Vol 1987 (Supplement 8) ◽  
pp. 141-163 ◽  
Author(s):  
M. M. MAREEL ◽  
F. M. VAN ROY ◽  
L. M. MESSIAEN ◽  
E. R. BOGHAERT ◽  
E. A. BRUYNEEL
Keyword(s):  
Quantitative Analysis ◽  
Qualitative And Quantitative Analysis ◽  
Tumour Invasion ◽  
Qualitative And Quantitative

scholarly journals A Review on Chromatography—Mass Spectrometry Applications on Anthocyanin and Ellagitannin Metabolites of Blackberries and Raspberries

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2150
Author(s):  
Dilip K. Rai ◽  
Katerina Tzima
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Health Effects ◽  
Analytical Techniques ◽  
In Vitro Models ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Chromatography Mass Spectrometry

Berries have been widely assessed for their beneficial health effects, predominately due to their high (poly)phenol content of anthocyanins and ellagitannins. After ellagitannins and ellagic acid are metabolized by the gut microbiome, a class of compounds known as urolithins are produced, which exert potential advantageous health effects. Anthocyanins, on the other hand, undergo a complex metabolic pathway after their interaction with microbial and endogenous enzymes, forming a broad range of metabolites and catabolic products. In most cases, in vitro models and cell lines are used to generate metabolites, whereas their assessment in vivo is currently limited. Thus far, several analytical methods have been developed for the qualitative and quantitative analysis of phenolic metabolites in berries, including liquid chromatography, mass spectrometry, and other hyphenated techniques, and have been undoubtedly valuable tools for the detailed metabolite characterization and profiling. In this review, a compilation of studies providing information on the qualitative and quantitative analysis of (poly)phenol metabolites in blackberries and raspberries after the utilization of in vitro and in vivo methods is presented. The different analytical techniques employed are assessed, focusing on the fate of the produced metabolic compounds in order to provide evidence on their characteristics, formation, and beneficial effects.

scholarly journals Qualitative and quantitative analysis of chromosome banding induced in vitro

Hereditas ◽  
2009 ◽  
Vol 94 (1) ◽  
pp. 53-59 ◽  
Author(s):  
MOGENS RøNNE ◽  
KIRSTEN VANG NIELSEN ◽  
OLE ANDERSEN
Keyword(s):  
Quantitative Analysis ◽  
Chromosome Banding ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative

Advancements in practical and scientific bioanalytical approaches to metabolism studies in drug development

Bioanalysis ◽  
2021 ◽  
Author(s):  
Dipali Sonawane ◽  
Anuradha Reddy ◽  
Tarang Jadav ◽  
Amit K Sahu ◽  
Rakesh K Tekade ◽  
...  
Keyword(s):  
Drug Development ◽  
Development Programs ◽  
Qualitative And Quantitative Analysis ◽  
Detailed Understanding ◽  
Qualitative And Quantitative ◽  
The Past ◽  
Recent Advancement ◽  
Bioanalytical Techniques

Advancement in metabolism profiling approaches and bioanalytical techniques has been revolutionized over the last two decades. Different in vitro and in vivo approaches along with advanced bioanalytical techniques are enabling the accurate qualitative and quantitative analysis of metabolites. This review summarizes various modern in vitro and in vivo approaches for executing metabolism studies with special emphasis on the recent advancement in the field. Advanced bioanalytical techniques, which can be employed in metabolism studies, have been discussed suggesting their particular application based on specific study objectives. This article can efficiently guide the researchers to scientifically plan metabolism studies and their bioanalysis during drug development programs taking advantage of a detailed understanding of instances of failure in the past.

scholarly journals Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor

2021 ◽  
Vol 4 (1) ◽  
pp. 8-18
Author(s):  
Gea Ocktiah Palensina ◽  
Leni Rosita ◽  
Zuraida Sagala
Keyword(s):  
Quantitative Analysis ◽  
Mobile Phase ◽  
Enzyme Inhibitor ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Extractive Substances ◽  
Ic50 Values ◽  
Rf Values ◽  
Tyrosinase Enzyme

Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.

scholarly journals Qualitative and Quantitative Analysis of Intracanal Delivery Systems of Calcium Hydroxide -An In-Vitro Study

2013 ◽  
Vol 3 (1) ◽  
pp. 8-14
Author(s):  
Shankar Narayan G ◽  
Ranga reddy D.S ◽  
Venkatesan S M ◽  
Veniashok B ◽  
Duraivel D
Keyword(s):  
Quantitative Analysis ◽  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Delivery Systems ◽  
Vitro Study ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative

scholarly journals Qualitative and quantitative analysis of buffalo ovaries, follicles and oocytes in view of in vitro production of embryos

2012 ◽  
Vol 40 (1-2) ◽  
pp. 23-27
Author(s):  
MAMY Khandoker ◽  
N Jahan ◽  
L Asad ◽  
SAM Hoque ◽  
S Ahmed ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Genital Tract ◽  
Qualitative And Quantitative Analysis ◽  
In Vitro Production ◽  
Qualitative And Quantitative ◽  
Length Width ◽  
The Right ◽  
Vitro Production ◽  
Weight Number

This study was conducted to evaluate the buffalo ovaries, follicles and cumulus-oocyte-complexes (COCs) with the view of in vitro production (IVP) of embryos. Buffalo (Bubalis bubalis) ovaries were collected from local slaughterhouse and categorized according to the position of ovaries in the genital tract (left vs. right) and presence or absence of corpus luteum (CL). Moreover, collected ovaries were also evaluated for length, width and weight, number of follicles, aspirated follicles and state of COCs. The length (cm) of right ovary (2.32±0.06) was significantly (P<0.05) higher than left ones (2.14±0.05). Number of follicles were higher (P<0.05) in the left (7.25±0.31) compare to the right (6.22±0.32) ovaries. Other parameters, including width, weight and number of COC aspirated did not differ significantly (P<0.05) between right and left ovaries. The length, width and weight of ovaries with CL were higher (P<0.05) whereas, number of observed follicles, aspirated follicles, number of COCs and number of normal COCs were significantly (P<0.05) higher in ovaries without CL than their counter parts.DOI: http://dx.doi.org/10.3329/bjas.v40i1-2.10786Bang. J. Anim. Sci. 2011. 409 (1-2): 23-27

Antigen shedding from the surface of the infective stage larvae of Dirofilaria immitis

Parasitology ◽  
1989 ◽  
Vol 99 (1) ◽  
pp. 89-97 ◽  
Author(s):  
M. S. Ibrahim ◽  
W. K. Tamashiro ◽  
D. A. Moraga ◽  
A. L. Scott
Keyword(s):  
Dirofilaria Immitis ◽  
Culture Medium ◽  
Infective Stage ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Sds Page ◽  
Immune Attack ◽  
Cuticular Surface

SUMMARYA qualitative and quantitative analysis was made of the release of surface-associated molecules from developing Dirofilaria immitis infective-stage larvae (L3). D. immitis L3s were labelled with 125I using an Iodogen catalysed reaction and either maintained in culture or placed in chambers that were implanted into Lewis rats. The larvae released 10–20% of the labelled material each day during the first 4 days of in vitro and in vivo development. The loss of surface-labelled peptides from developing larvae corresponded with an increase in the amount of trichloroacetic acid-precipitable radioactivity found in the culture medium. SDS—PAGE analysis of the labelled material showed that the same 35 and 6 kDa components found in larval extracts were shed into culture medium by the developing parasites. Metabolic labelling studies and experiments in which larvae were labelled after different times in culture indicated that, once released, the surfaceassociated molecules were not replaced, and that this net loss of surface peptides resulted in a reduction in the antigenic potential of the cuticular surface. Antibodies from both immunized rabbits and naturally infected dogs immunoprecipitated the 35 kDa component. In contrast, the 6 kDa molecule was not recognized by the antibodies in any of the sera tested. Shedding of surface peptides and reducing surface antigenicity may represent mechanisms by which D. immitis infective-stage larvae evade immune attack.

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