scholarly journals Dissecting lipid droplet biology with coherent Raman scattering microscopy

2021 ◽  
Vol 135 (5) ◽  
Author(s):  
Tao Chen ◽  
Ahmet Yavuz ◽  
Meng C. Wang
Keyword(s):  
Raman Scattering ◽  
Lipid Droplets ◽  
Quantitative Imaging ◽  
Live Cells ◽  
Label Free ◽  
Genetic Screens ◽  
Spatiotemporal Resolution ◽  
Coherent Raman Scattering ◽  
Coherent Raman

ABSTRACT Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated with a variety of diseases. The content level, composition and mobility of LDs are crucial for their physiological and pathological functions, and these different parameters of LDs are subject to regulation by genetic factors and environmental inputs. Coherent Raman scattering (CRS) microscopy utilizes optical nonlinear processes to probe the intrinsic chemical bond vibration, offering label-free, quantitative imaging of lipids in vivo with high chemical specificity and spatiotemporal resolution. In this Review, we provide an overview over the principle of CRS microscopy and its application in tracking different parameters of LDs in live cells and organisms. We also discuss the use of CRS microscopy in genetic screens to discover lipid regulatory mechanisms and in understanding disease-related lipid pathology.

scholarly journals Label-free in vivo analysis of intracellular lipid droplets in the oleaginous microalga Monoraphidium neglectum by coherent Raman scattering microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Daniel Jaeger ◽  
Christian Pilger ◽  
Henning Hachmeister ◽  
Elina Oberländer ◽  
Robin Wördenweber ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Lipid Droplets ◽  
Label Free ◽  
Intracellular Lipid ◽  
Coherent Raman Scattering ◽  
In Vivo Analysis ◽  
Coherent Raman

scholarly journals Coherent Raman Scattering Microscopy in Oncology Pharmacokinetic Research

2021 ◽  
Vol 12 ◽  
Author(s):  
Junjie Zeng ◽  
Wenying Zhao ◽  
Shuhua Yue
Keyword(s):  
Raman Scattering ◽  
High Speed ◽  
Cancer Drug ◽  
Cancer Drugs ◽  
High Speed Imaging ◽  
Coherent Raman Scattering ◽  
Anti Cancer ◽  
Coherent Raman

The high attrition rates of anti-cancer drugs during clinical development remains a bottleneck problem in pharmaceutical industry. This is partially due to the lack of quantitative, selective, and rapid readouts of anti-cancer drug activity in situ with high resolution. Although fluorescence microscopy has been commonly used in oncology pharmacological research, fluorescent labels are often too large in size for small drug molecules, and thus may disturb the function or metabolism of these molecules. Such challenge can be overcome by coherent Raman scattering microscopy, which is capable of chemically selective, highly sensitive, high spatial resolution, and high-speed imaging, without the need of any labeling. Coherent Raman scattering microscopy has tremendously improved the understanding of pharmaceutical materials in the solid state, pharmacokinetics of anti-cancer drugs and nanocarriers in vitro and in vivo. This review focuses on the latest applications of coherent Raman scattering microscopy as a new emerging platform to facilitate oncology pharmacokinetic research.

scholarly journals Quantitative imaging of lipid droplets in single cells

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 753-765 ◽  
Author(s):  
Anushka Gupta ◽  
Gabriel F. Dorlhiac ◽  
Aaron M. Streets
Keyword(s):  
Lipid Droplets ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Cell Level ◽  
Spatial Characterization ◽  
Coherent Raman Scattering ◽  
Coherent Raman ◽  
Computational Image Analysis ◽  
Non Destructive

Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.

scholarly journals Mapping Solvation Heterogeneity in Live Cells by Hyperspectral Stimulated Raman Scattering Microscopy

2020 ◽  
Author(s):  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Raman Scattering ◽  
Near Infrared ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
Environmental Variance ◽  
Dynamic Matrix ◽  
Coherent Raman ◽  
Living Organisms ◽  
Stimulated Raman

<p>Water provides a dynamic matrix in which all biochemical processes occur in living organisms. The structure and dynamics of intracellular water constitute the cornerstone for understanding all aspects of cellular function. Fundamentally, direct visualization of subcellular solvation heterogeneity is essential but remains challenging with commonly used NMR methods due to poor spatial resolution. To explore this question, we demonstrate a vibrational-shift imaging approach by combining the spectral-focusing hyperspectral stimulated Raman scattering (hsSRS) technique with an environmentally-sensitive nitrile probe. The sensing ability of a near-infrared nitrile-containing molecule is validated in the solution phase, microscopic droplets and cellular environments. Finally, we quantitatively measure the subcellular solvation variance between the cytoplasm (29.5%, S.E. 1.8%) and the nucleus (57.3%, S.E. 1.0%), which is in good agreement with previous studies. This work sheds light on heterogenous solvation in live systems using coherent Raman microscopy and opens up new avenues to explore environmental variance in complex systems with high spatiotemporal resolution.</p>

Mapping Solvation Heterogeneity in Live Cells by Hyperspectral Stimulated Raman Scattering Microscopy

2019 ◽  
Author(s):  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Raman Scattering ◽  
Near Infrared ◽  
Stimulated Raman Scattering ◽  
Free Water ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
Environmental Variance ◽  
Dynamic Matrix ◽  
Coherent Raman ◽  
Stimulated Raman

<div><div><div><p>Water provides a dynamic matrix in which all biochemical processes occur in living organisms. The structure and dynamics of intracellular water constitute the cornerstone for understanding all aspects of cellular function. Fundamentally, direct visualization of subcellular solvation heterogeneity is essential but remains challenging with commonly used NMR methods due to poor spatial resolution. To explore this question, we demonstrate a vibrational-shift imaging approach by combining the spectral-focusing hyperspectral stimulated Raman scattering (hsSRS) technique with an environmentally-sensitive nitrile probe. The sensing ability of a near-infrared nitrile-containing molecule is validated in the solution phase, microscopic droplets and cellular environments. Finally, we quantitatively measure the subcellular solvation variance in the degree of free water content between the cytoplasm (29.7%, S.E. 1.1%) and the nucleus (56.4%, S.E. 1.3%), which is in good agreement with previous studies. This work sheds light on mapping the heterogenous solvation in live systems using coherent Raman microscopy and opens up new avenues to explore environmental variance in complex systems with high spatiotemporal resolution.</p></div></div></div>

scholarly journals Mapping Solvation Heterogeneity in Live Cells by Hyperspectral Stimulated Raman Scattering Microscopy

2020 ◽  
Author(s):  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Raman Scattering ◽  
Near Infrared ◽  
Stimulated Raman Scattering ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
Environmental Variance ◽  
Dynamic Matrix ◽  
Coherent Raman ◽  
Living Organisms ◽  
Stimulated Raman

<p>Water provides a dynamic matrix in which all biochemical processes occur in living organisms. The structure and dynamics of intracellular water constitute the cornerstone for understanding all aspects of cellular function. Fundamentally, direct visualization of subcellular solvation heterogeneity is essential but remains challenging with commonly used NMR methods due to poor spatial resolution. To explore this question, we demonstrate a vibrational-shift imaging approach by combining the spectral-focusing hyperspectral stimulated Raman scattering (hsSRS) technique with an environmentally-sensitive nitrile probe. The sensing ability of a near-infrared nitrile-containing molecule is validated in the solution phase, microscopic droplets and cellular environments. Finally, we quantitatively measure the subcellular solvation variance between the cytoplasm (29.5%, S.E. 1.8%) and the nucleus (57.3%, S.E. 1.0%), which is in good agreement with previous studies. This work sheds light on heterogenous solvation in live systems using coherent Raman microscopy and opens up new avenues to explore environmental variance in complex systems with high spatiotemporal resolution.</p>

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