scholarly journals Unusual Farnesyl Pyrophosphate Synthetase Reaction of an Artificial Substrate with Ni2+

1986 ◽  
Vol 15 (11) ◽  
pp. 1885-1888 ◽  
Author(s):  
Yuji Maki ◽  
Gotaro Watanabe ◽  
Akio Saito ◽  
Tanetoshi Koyama ◽  
Kyozo Ogura
Keyword(s):  
Artificial Substrate ◽  
Farnesyl Pyrophosphate

ChemInform Abstract: Unusual Farnesyl Pyrophosphate Synthetase Reaction of an Artificial Substrate with Ni2+.

ChemInform ◽  
1987 ◽  
Vol 18 (23) ◽  
Author(s):  
Y. MAKI ◽  
G. WATANABE ◽  
A. SAITO ◽  
T. KOYAMA ◽  
K. OGURA
Keyword(s):  
Artificial Substrate ◽  
Farnesyl Pyrophosphate

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

The Preparation of an Artificial Reagent for the One-Stage Factor VIII Assay

1970 ◽  
Vol 23 (02) ◽  
pp. 306-312 ◽  
Author(s):  
D Nyman
Keyword(s):  
Factor Viii ◽  
Artificial Substrate ◽  
One Stage ◽  
The One

SummaryA method for preparation of an artificial substrate for the one-stage factor VIII assay is described. Standardization of the test is given. The method is specific and reproducible.

Cloning and characterization of Farnesyl pyrophosphate synthase gene from Anoectochilus

2019 ◽  
Vol 52 (3) ◽  
Author(s):  
Lin Yang ◽  
Jun Cheng Zhang ◽  
Wan Chen Li ◽  
Jing Tao Qu ◽  
Hao Qiang Yu ◽  
...  
Keyword(s):  
Farnesyl Pyrophosphate ◽  
Farnesyl Pyrophosphate Synthase

In Situ Coupling of Catalytic Centers into Artificial Substrate Mesochannels as Super‐Active Metalloenzyme Mimics

Small ◽  
2021 ◽  
pp. 2101455
Author(s):  
Jian Yang ◽  
Ke Li ◽  
Chunzhong Li ◽  
Jinlou Gu
Keyword(s):  
Artificial Substrate

Infection of cucumber seedling roots by Pseudomonas amygdali pv. lachrymans following artificial substrate inoculation

2021 ◽  
Author(s):  
Shengping Zhang ◽  
Xianglong Meng ◽  
Ying Cheng ◽  
Yanan Wang ◽  
Tongle Hu ◽  
...  
Keyword(s):  
Artificial Substrate ◽  
Cucumber Seedling ◽  
Seedling Roots ◽  
Pseudomonas Amygdali

Brain Isoprenoids Farnesyl Pyrophosphate and Geranylgeranyl Pyrophosphate are Increased in Aged Mice

2012 ◽  
Vol 46 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Gero P. Hooff ◽  
W. Gibson Wood ◽  
Ji-Hyun Kim ◽  
Urule Igbavboa ◽  
Wei-Yi Ong ◽  
...  
Keyword(s):  
Farnesyl Pyrophosphate ◽  
Geranylgeranyl Pyrophosphate ◽  
Aged Mice

scholarly journals An artificial test substrate for evaluating electron microscopic immunocytochemical labeling reactions.

1987 ◽  
Vol 35 (8) ◽  
pp. 909-916 ◽  
Author(s):  
G D Gagne ◽  
M F Miller
Keyword(s):  
Electron Microscope ◽  
Hepatitis B ◽  
Horseradish Peroxidase ◽  
Polyclonal Antibodies ◽  
Hepatitis B Surface Antigen ◽  
Immunogold Labeling ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
Electron Microscopic ◽  
Immunocytochemical Studies

We describe an artificial substrate system for optimization of labeling parameters in electron microscope immunocytochemical studies. The system involves use of blocks of glutaraldehyde-polymerized BSA into which a desired antigen is incorporated by a simple soaking procedure. The resulting antigen-impregnated artificial substrate can then be fixed and embedded identically to a piece of tissue. The BSA substrate can also be dried and then sectioned for immunolabeling with or without chemical fixation and without exposing the antigen to dehydrating agents and embedding resins. The effects of various fixation and embedding procedures can thus be evaluated separately. Other parameters affecting immunocytochemical labeling, such as antibody and conjugate concentration, can also be evaluated. We used this system, along with immunogold labeling, to determine quantitatively the optimal fixation and embedding conditions for labeling of hepatitis B surface antigen (HbsAg), human IgG, and horseradish peroxidase. Using unfixed and unembedded HBsAg, we were able to detect antigen concentrations below 20 micrograms/ml. We have shown that it is not possible to label HBsAg within resin-embedded cells using conventional aldehyde fixation protocols and polyclonal antibodies.

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