Development of an in Vitro Drug Release Assay of PEGylated Liposome Using Bovine Serum Albumin and High Temperature

Abstract In this work, first, copper oxide nanoparticles (CUO NPs) were synthesized by physical methods and then coated with the bovine serum albumin (BSA) via biologically meditated minerals to form CUO@BSA NPs. Finally, curcumin (CUR) as an anticancer drug were immobilized on the surface of CUO@BSA NPS. The properties of CUO@BSA-CUR NPS were investigated by FTIR, UV-Vis, TEM, and AFM spectroscopes. It was found that the synthesized CUO@BSA-CUR nanoparticles were spherical with a particle size of 20 to 30 nm and have a sustained release of CUR at 37°C in buffer solution. Also, the result of release in biological environment showed that maximum drug release rate for this nanocarrier in pH 7.4 was measured 75% after 48 hours. The cytotoxicity of CUO@BSA-CUR on MDA-MB-231 cell line was studied. The results showed that CUO@BSA-CUR nanoparticles have significant cytotoxic activity on this cell line, while the results of MTT assay indicated the CUO@BSA NPs have no toxicity effect on the cancer cells.

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PREPARATION AND IN VITRO EVALUATION OF METOPROLOL-LOADED BOVINE SERUM ALBUMIN NANOPARTICLES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i1.39738 ◽

2021 ◽

pp. 213-217

Author(s):

SHIVA KUMAR YELLANKI ◽

SAI MANOJ A ◽

MANGILAL T

Keyword(s):

Particle Size ◽

Bovine Serum Albumin ◽

Drug Release ◽

Zeta Potential ◽

Serum Albumin ◽

Bovine Serum ◽

Polymer Concentration ◽

Entrapment Efficiency ◽

Nanoprecipitation Method

Objective: The aim of the present research was to prepare metoprolol-loaded nanospheres. Metoprolol-loaded bovine albumin nanospheres were prepared by nanoprecipitation method. Metoprolol is beta-1-adrenergic receptor inhibitor specific to cardiac cells, thus producing negative chronotropic and ionotropic effect. Methods: Metoprolol nanospheres were prepared by nanoprecipitation method, using bovine serum albumin as polymer. The prepared nanospheres are evaluated for particle size evaluation, drug entrapment efficiency, and zeta potential. Drug-excipient compatibility was determined using Fourier-transform infrared spectroscopy. In vitro release and solubility of the drug from nanoparticles were determined. Results: The particle size of prepared metoprolol nanospheres was found to be always less than 200 nm. Maximum particle size was found to be 196±2.03 nm of batch 4 nanoparticles. Entrapment efficiency of prepared nanospheres was above 80% and maximum percentage entrapment efficiency was found to be 80.4±0.51%. It was found that the percentage entrapment efficiency and drug release were extended with increase in polymer concentration. Zeta potential of the optimized formulation was found to be −20.4 mV. In vitro drug release studies have shown the prolonged release of 94.5±0.54 up to 10 h. Drug release rate is extended with an increase in polymer concentration. Conclusion: Results have concluded that the albumin nanospheres loaded with metoprolol have reduced the blood pressure within 24 h and the prepared nanospheres are effective compared to other formulations and drug delivery.

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Examining the effect of bovine serum albumin on the properties and drug release behavior of β-lactoglobulin-derived amyloid fibril-based hydrogels

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2021.06.003 ◽

2021 ◽

Vol 184 ◽

pp. 79-91

Author(s):

Su-Chun How ◽

Ta-Hsien Lin ◽

Chun-Chao Chang ◽

Steven S.-S. Wang

Keyword(s):

Bovine Serum Albumin ◽

Drug Release ◽

Serum Albumin ◽

Bovine Serum ◽

Amyloid Fibril ◽

Release Behavior ◽

Drug Release Behavior ◽

Β Lactoglobulin

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A Precipitin-like Reaction of the Hemolymph of the Lobster Homarus americanus

Journal of the Fisheries Research Board of Canada ◽

10.1139/f69-127 ◽

1969 ◽

Vol 26 (5) ◽

pp. 1392-1397 ◽

Cited By ~ 10

Author(s):

James E. Stewart ◽

Diane M. Foley

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Serum Proteins ◽

Homarus Americanus ◽

Test Period ◽

Fluorescent Material

The levels of fluorescent material in the hemolymph of lobsters injected with serum proteins from lobster hemolymph labelled with fluorescein remained relatively constant over a 6-day test period; the levels in lobsters injected with bovine serum albumin labelled with fluorescein declined rapidly. A precipitin-like reaction was observed when lobster hemolymph serum was titrated with bovine serum albumin in vitro.

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Preparation and in vitro photodynamic activities of novel axially substituted silicon (IV) phthalocyanines and their bovine serum albumin conjugates

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2006.01.075 ◽

2006 ◽

Vol 16 (9) ◽

pp. 2450-2453 ◽

Cited By ~ 42

Author(s):

Xiong-Jie Jiang ◽

Jian-Dong Huang ◽

Yu-Jiao Zhu ◽

Fen-Xiang Tang ◽

Dennis K.P. Ng ◽

...

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum

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Amuchina Gel Xgerm hand rub in vitro virucidal activity against SARS-CoV-2

Future Microbiology ◽

10.2217/fmb-2021-0128 ◽

2021 ◽

Author(s):

Alessandra Capezzone de Joannon ◽

Angela Testa ◽

Natalie Falsetto ◽

Michela Procaccini ◽

Lorella Ragni

Keyword(s):

Detection Limit ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Virucidal Activity ◽

European Standard ◽

Enveloped Viruses ◽

Prevention Measure ◽

Study Product

Aim: Ethanol is highly effective at inactivating enveloped viruses, including SARS-CoV-2. The aim of this study is to evaluate the virucidal activity of Amuchina Gel Xgerm (74% ethanol) against SARS-CoV-2, according to the European Standard EN14476:2013+A2:2019. Materials & methods: Virucidal activity of the study product was evaluated against SARS-CoV-2 strain USAWA1/2020 in suspension, in the presence of 0.3 g/l of bovine serum albumin. Results: The log10 reduction of SARS-CoV-2 in the presence of bovine serum albumin was ≥4.11 ± 0.12 after 30 s of exposure to the study product (80% dilution). Cytotoxicity was observed in the 100 dilution, affecting the detection limit by 1 log10. Conclusion: Virucidal activity against SARS-CoV-2 supports the effectiveness of this alcohol-based formulation as a prevention measure for COVID-19 illness.

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