scholarly journals Mechanism of Action of Panaxytriol on Midazolam 1′-Hydroxylation and 4-Hydroxylation Mediated by CYP3A in Liver Microsomes and Rat Primary Hepatocytes

2015 ◽  
Vol 38 (10) ◽  
pp. 1470-1477 ◽  
Author(s):  
Fang He ◽  
Wen Zhang ◽  
Caiwen Zeng ◽  
Chunhua Xia ◽  
Yuqing Xiong ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Liver Microsomes ◽  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes

scholarly journals Investigation of sphingosin-1-phosphate-triggered matriptase activation using a rat primary hepatocyte model

2019 ◽  
Vol 67 (4) ◽  
pp. 578-587
Author(s):  
Réka Fanni Barna ◽  
Judit Mercédesz Pomothy ◽  
Zsuzsanna Paréj ◽  
Erzsébet Pásztiné Gere
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Cell Viability ◽  
Mechanism Of Action ◽  
Sandwich Elisa ◽  
Colorimetric Method ◽  
Redox Status ◽  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes ◽  
Sphingosine 1 Phosphate

Sphingosine-1-phosphate (S1P) has been reported as a matriptase activator. The aim of this study was to reveal if S1P can influence hepcidin production. Furthermore, we investigated how S1P can affect the viability and the redox status of primary hepatocytes. Rat primary hepatocytes were cultivated for 72 h and were treated with 50, 200, 1000 ng/ml S1P. Cell-free supernatants were collected every 24 h. Cell viability was tested by a colorimetric method using tetrazolium compound (MTS). The hepcidin levels in the cell-free supernatants were examined with hepcidin sandwich ELISA to determine the effect of S1P on the hepcidin-modulating ability of matriptase. In order to estimate the extent of S1P-generated oxidative stress, extracellular H2O2 measurements were performed by the use of fluorescent dye. Based on the findings, S1P treatment did not cause cell death for 72 h at concentrations up to 1000 ng/ml. S1P did not influence the extracellular H2O2 production for 72 h. The hepcidin levels were significantly suppressed in hepatocytes exposed to S1P treatment. Further studies would be needed to explore the exact mechanism of action of S1P.

Butein Up-Regulates the Expression of the π Class of GlutathioneS-Transferase in Rat Primary Hepatocytes through the ERK/AP-1 Pathway

2010 ◽  
Vol 58 (16) ◽  
pp. 8994-9000 ◽  
Author(s):  
Hebron C. Chang ◽  
Haw-Wen Chen ◽  
Hui-Shan Tung ◽  
Kai-Li Liu ◽  
Chia-Wen Tsai ◽  
...  
Keyword(s):  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes
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