Manipulation of Charge States of Biopolymer Ions by Atmospheric Pressure Ion/Molecule Reactions Implemented in an Extractive Electrospray Ionization Source

2007 ◽  
Vol 13 (4) ◽  
pp. 273-279 ◽  
Author(s):  
Huanwen Chen ◽  
David Touboul ◽  
Matthias Conradin Jecklin ◽  
Jian Zheng ◽  
Mingbiao Luo ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Charge State ◽  
Atmospheric Pressure ◽  
Ambient Pressure ◽  
Quadrupole Ion Trap ◽  
Ionization Source ◽  
Ion Molecule Reactions ◽  
Multiply Charged ◽  
Charge States ◽  
Low Charge

A home-made extractive electrospray ionization source is coupled to an linear quadrupole ion trap mass spectrometer to investigate ion/molecule reactions of biopolymers at ambient pressure. Multiply charged biopolymers such as peptides and proteins generated in an electrospray are easily reduced to a low charge state by the atmospheric pressure ion/molecule reactions occurring between the multiply charged ions and a strong basic reagent sprayed in neutral form into the electrospray plume. The charge state of the biopolymer ions can be manipulated by controlling the amount of the basic reagent. The production of biopolymer ions with low charge states results in a substantial improvement of sensitivity and reduced spectral congestion in ESI-MS. This is of importance for biopolymer mixture analysis and could have promising applications in proteomics.

Rectilinear Ion Trap Mass Spectrometer with Atmospheric Pressure Interface and Electrospray Ionization Source

2006 ◽  
Vol 78 (3) ◽  
pp. 718-725 ◽  
Author(s):  
Qingyu Song ◽  
Sameer Kothari ◽  
Michael A. Senko ◽  
Jae C. Schwartz ◽  
Jonathan W. Amy ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectrometer ◽  
Atmospheric Pressure ◽  
Ion Trap ◽  
Ionization Source ◽  
Ion Trap Mass Spectrometer

Desorption electrospray ionization (DESI) with atmospheric pressure ion mobility spectrometry for drug detection

The Analyst ◽  
2014 ◽  
Vol 139 (7) ◽  
pp. 1740-1750 ◽  
Author(s):  
Kristyn M. Roscioli ◽  
Jessica A. Tufariello ◽  
Xing Zhang ◽  
Shelly X. Li ◽  
Gilles H. Goetz ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Ion Mobility ◽  
Atmospheric Pressure ◽  
Drift Tube ◽  
Ambient Pressure ◽  
Desorption Electrospray Ionization ◽  
Direct Analysis ◽  
Drug Detection ◽  
Pharmaceutical Samples ◽  
P Desorption

Desorption electrospray ionization (DESI) was coupled to an ambient pressure drift tube ion mobility time-of-flight mass spectrometer (IM-TOFMS) for the direct analysis of active ingredients in pharmaceutical samples.

scholarly journals A LC-MS/MS method with electrospray ionization and atmospheric pressure chemical ionization source for analysis of pesticides in hemp

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Avinash Dalmia ◽  
Erasmus Cudjoe ◽  
Jacob Jalali ◽  
Feng Qin
Keyword(s):  
Solvent Extraction ◽  
Sample Preparation ◽  
Electrospray Ionization ◽  
Atmospheric Pressure ◽  
Chemical Ionization ◽  
Atmospheric Pressure Chemical Ionization ◽  
Ionization Source ◽  
Limit Of Quantitation ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical

Abstract Background Pesticide testing for hemp has traditionally focused on techniques like QuEChERS with dSPE and SPE which demand time-consuming sample preparation, typically resulting in poor recovery rates for some pesticides, and requires the use of both LC-MS/MS and GC-MS/MS based instruments to cover the analysis for all regulated pesticides. In this study, we describe a streamlined approach for working with LC-MS/MS featuring a dual electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources using solvent extraction for faster and easier sample preparation and with 80–120% recovery for the analysis of all of 66 pesticides (regulated by California state in cannabis) with low detection limits in hemp. Methods A simple solvent extraction with acetonitrile was used to extract pesticides from hemp. A LC-MS/MS system with dual ESI and APCI source was used to determine sensitivity for the analysis of 66 pesticides in hemp matrix, 62 pesticides were analyzed using an 18-min LC-MS/MS method with an ESI source and the other 4 pesticides were measured using a 6-min LC-MS/MS method with an APCI source. Results The limit of quantitation (LOQ) of all 66 pesticides in hemp was in the range of 0.0025–0.1 μg/g which was well below the California state action limits of these analytes in cannabis products. A simple, fast, and cost-effective solvent extraction method was used for sample preparation to get good recovery in the range of 80–120% with RSD less than 20%. The unique ionization mechanism of chlorinated pesticides such as pentachloronitrobenzene using the LC-MS/MS system with an APCI source was elucidated. The proficiency test report generated with the LC-MS/MS method showed acceptable results for all of 66 pesticides in hemp with all of th z scores less than 2 with no false positives and negatives. The stability data collected over 5 days showed RSD less than 20% for 66 pesticides in hemp, and this demonstrated the robustness of the LC-MS/MS system used in this work. Conclusions A LC-MS/MS method with dual ESI and APCI sources was developed for the analysis of 66 pesticides in hemp. The recovery of all pesticides from a hemp matrix was in the acceptable range of 80–120% with RSD less than 20%.

scholarly journals Comparison of the efficiency of photoionization at atmospheric pressure and electrospray ionization on the example of some aflatoxins and trichothecenes

2020 ◽  
Vol 64 (12) ◽  
pp. 113-119
Author(s):  
Aisylu Z. Mukharlyamova ◽  
◽  
Aleksandr M. Saifutdinov ◽  
Elvira R. Rakhmetova ◽  
Aygul G. Mukhammetshina ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Aflatoxin B1 ◽  
Atmospheric Pressure ◽  
Raw Materials ◽  
Food Products ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Ionization Source ◽  
Mass Spectrometric Detector

The article presents the results of comparing the effectiveness of modern ionization methods used in chromatomass spectrometric analysis: photoionization at atmospheric pressure (APPI) and electrospray ionization (ESI) for the determination of aflatoxin B1 and T-2 toxins related to the main natural pollutants of agricultural feed, food raw materials and food products. Aflatoxin B1 is a member of the aflatoxin family, which in their chemical structure are derivatives of substituted coumarins or furocoumarins. The peculiarity of aflatoxins is that in the process of technological or culinary processing, they are practically not destroyed, while being the strongest hepatocancerogens that cause irreversible liver damage. T-2 toxin refers to trichothecene mycotoxins, in the structure of which there is a trichothecene ring containing a multiple bond and an epoxy group. Most trichothecene mycotoxins are slightly toxic compounds, but T-2 toxin is deadly, having a negative effect on immunocompetent organs, hematopoietic organs, and the gastrointestinal tract, increasing the risk of internal hemorrhage. Due to the impossibility of preventing contamination of agricultural feed, food raw materials and food products with mycotoxins, their strict control is necessary. Chromatomass spectrometry is one of the methods that provide high sensitivity and reliability for the determination of mycotoxins Methods of ionization sources, such as electrospray and photoionization at atmospheric pressure during mass spectrometric detection of mycotoxins by high-performance liquid chromatography (HPLC-MS/MS), are evaluated. In order to increase the sensitivity of the determinations, the optimal conditions of the quadrupole-time-of-flight mass spectrometric detector were selected and the maximum intensity responses of the analytes were obtained. During the experiment, it was found that the intensity of the signal with a photoionization source obtained by analyzing the T-2 toxin is higher than the signal obtained by using an electrospray ionization source.

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