scholarly journals Examination of transpositional activity of nDart1 at different stages of rice development

2011 ◽  
Vol 86 (3) ◽  
pp. 215-219 ◽  
Author(s):  
Mika Hayashi-Tsugane ◽  
Masahiko Maekawa ◽  
Hirokazu Kobayashi ◽  
Shigeru Iida ◽  
Kazuo Tsugane
Keyword(s):  
Transpositional Activity

Transpositional activity of IS986 in Mycobacterium smegmatis

Gene ◽  
1993 ◽  
Vol 130 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Ndingsa G. Fomukong ◽  
Jeremy W. Dale
Keyword(s):  
Mycobacterium Smegmatis ◽  
Transpositional Activity

scholarly journals Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

2020 ◽  
Vol 11 ◽  
Author(s):  
Elena Yushkova
Keyword(s):  
Dna Repair ◽  
Genome Stability ◽  
Specific Response ◽  
Dna Breaks ◽  
Transgenerational Effects ◽  
Transpositional Activity ◽  
Effective Strategies ◽  
Radiation Induced ◽  
Mechanisms Of Adaptation ◽  
Transposon Activity

The study of the genetic basis of the manifestation of radiation-induced effects and their transgenerational inheritance makes it possible to identify the mechanisms of adaptation and possible effective strategies for the survival of organisms in response to chronic radioactive stress. One persistent hypothesis is that the activation of certain genes involved in cellular defense is a specific response of the cell to irradiation. There is also data indicating the important role of transposable elements in the formation of radiosensitivity/radioresistance of biological systems. In this work, we studied the interaction of the systems of hobo transposon activity and DNA repair in the cell under conditions of chronic low-dose irradiation and its participation in the inheritance of radiation-induced transgenerational instability in Drosophila. Our results showed a significant increase of sterility and locus-specific mutability, a decrease of survival, fertility and genome stability (an increase the frequency of dominant lethal mutations and DNA damage) in non-irradiated F1/F2 offspring of irradiated parents with dysfunction of the mus304 gene which is responsible for excision and post-replicative recombination repair and repair of double-stranded DNA breaks. The combined action of dysfunction of the mus309 gene and transpositional activity of hobo elements also led to the transgenerational effects of irradiation but only in the F1 offspring. Dysfunction of the genes of other DNA repair systems (mus101 and mus210) showed no visible effects inherited from irradiated parents subjected to hobo transpositions. The mei-41 gene showed specificity in this type of interaction, which consists in its higher efficiency in sensing events induced by transpositional activity rather than irradiation.

scholarly journals Involvement of ςS in Starvation-Induced Transposition of Pseudomonas putidaTransposon Tn4652

2001 ◽  
Vol 183 (18) ◽  
pp. 5445-5448 ◽  
Author(s):  
Heili Ilves ◽  
Rita Hõrak ◽  
Maia Kivisaar
Keyword(s):  
Stationary Phase ◽  
Pseudomonas Putida ◽  
Sigma Factor ◽  
Mobile Element ◽  
Environmental Stresses ◽  
Mobile Elements ◽  
Present Evidence ◽  
Transpositional Activity ◽  
Specific Regulation

ABSTRACT Transpositional activity of mobile elements can be induced by different environmental stresses. Here, we present evidence that transposition of Tn4652 is elevated in stationary-phasePseudomonas putida and suppressed in an isogenic ςS-defective strain. We demonstrate that transcription from the Tn4652 transposase promoter is controlled by the stationary-phase-specific sigma factor ςS. To our knowledge, this is the first example of direct stationary-phase-specific regulation of a mobile element transposase. Data presented in this report support the idea that activation of transposition under stressful conditions could be an inducible process.

scholarly journals The Left End of IS2: a Compromise between Transpositional Activity and an Essential Promoter Function That Regulates the Transposition Pathway

2004 ◽  
Vol 186 (3) ◽  
pp. 858-865 ◽  
Author(s):  
Leslie A. Lewis ◽  
Edruge Cylin ◽  
Ho Kyung Lee ◽  
Robert Saby ◽  
Wilson Wong ◽  
...  
Keyword(s):  
Second Step ◽  
Inverted Repeats ◽  
Sequence Information ◽  
Linear Element ◽  
Strong Promoter ◽  
Transpositional Activity ◽  
Hybrid Promoter ◽  
Optimal Function ◽  
Replicative Transposition ◽  
Novel Variation

ABSTRACT Cut-and-paste (simple insertion) and replicative transposition pathways are the two classical paradigms by which transposable elements are mobilized. A novel variation of cut and paste, a two-step transposition cycle, has recently been proposed for insertion sequences of the IS3 family. In IS2 this variation involves the formation of a circular, putative transposition intermediate (the minicircle) in the first step. Two aspects of the minicircle may involve its proposed role in the second step (integration into the target). The first is the presence of a highly reactive junction formed by the two abutted ends of the element. The second is the assembly at the minicircle junction of a strong hybrid promoter which generates higher levels of transposase. In this report we show that IS2 possesses a highly reactive minicircle junction at which a strong promoter is assembled and that the promoter is needed for the efficient completion of the pathway. We show that the sequence diversions which characterize the imperfect inverted repeats or ends of this element have evolved specifically to permit the formation and optimal function of this promoter. While these sequence diversions eliminate catalytic activity of the left end (IRL) in the linear element, sufficient sequence information essential for catalysis is retained by the IRL in the context of the minicircle junction. These data confirm that the minicircle is an essential intermediate in the two-step transposition pathway of IS2.

scholarly journals REM1, a New Type of Long Terminal Repeat Retrotransposon in Chlamydomonas reinhardtii

2005 ◽  
Vol 25 (23) ◽  
pp. 10628-10638 ◽  
Author(s):  
Mónica Pérez-Alegre ◽  
Alain Dubus ◽  
Emilio Fernández
Keyword(s):  
Long Terminal Repeat ◽  
Chlamydomonas Reinhardtii ◽  
Copy Number ◽  
Structural Features ◽  
Terminal Repeat ◽  
Phd Finger ◽  
Transpositional Activity ◽  
Regulatory Module ◽  
New Type ◽  
Foreign Dna Integration

ABSTRACT A new long terminal repeat (LTR) retrotransposon, named REM1, has been identified in the green alga Chlamydomonas reinhardtii. It was found in low copy number, highly methylated, and with an inducible transpositional activity. This retrotransposon is phylogenetically related to Ty3-gypsy LTR retrotransposons and possesses new and unusual structural features. A regulatory module, ORF3p, is present in an inverse transcriptional orientation to that of the polyprotein and contains PHD-finger and chromodomains, which might confer specificity of the target site and are highly conserved in proteins involved in transcriptional regulation by chromatin remodeling. By using different wild-type and mutant strains, we show that CrREM1 was active with a strong transcriptional activity and amplified its copy number in strains that underwent foreign DNA integration and/or genetic crosses. However, integration of CrREM1 was restricted to these events even though the expression of its full-length transcripts remained highly activated. A regulatory mechanism of CrREM1 retrotransposition which would help to minimize its deleterious effects in the host genome is proposed.

scholarly journals Tissue culture-induced transpositional activity of mPing is correlated with cytosine methylation in rice

2009 ◽  
Vol 9 (1) ◽  
pp. 91 ◽  
Author(s):  
Frédéric Ngezahayo ◽  
Chunming Xu ◽  
Hongyan Wang ◽  
Lily Jiang ◽  
Jinsong Pang ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cytosine Methylation ◽  
Transpositional Activity

Transposon display supports transpositional activity of P elements in species of the saltans group of Drosophila

2007 ◽  
Vol 86 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Nathalia de Setta ◽  
Ana Paula Pimentel Costa ◽  
Fabrício Ramon Lopes ◽  
Marie-Anne van Sluys ◽  
Cláudia Márcia Aparecida Carareto
Keyword(s):  
Transposon Display ◽  
Transpositional Activity ◽  
P Elements ◽  
Saltans Group

scholarly journals Sex and the TEs: transposable elements in sexual development and function in animals

Mobile DNA ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Corentin Dechaud ◽  
Jean-Nicolas Volff ◽  
Manfred Schartl ◽  
Magali Naville
Keyword(s):  
Transposable Elements ◽  
Sexual Reproduction ◽  
Dna Sequences ◽  
Sexual Development ◽  
Regulatory Sequences ◽  
New Host ◽  
Transpositional Activity ◽  
Evolutionary Success ◽  
And Function ◽  
Host Genes

Abstract Transposable elements are endogenous DNA sequences able to integrate into and multiply within genomes. They constitute a major source of genetic innovations, as they can not only rearrange genomes but also spread ready-to-use regulatory sequences able to modify host gene expression, and even can give birth to new host genes. As their evolutionary success depends on their vertical transmission, transposable elements are intrinsically linked to reproduction. In organisms with sexual reproduction, this implies that transposable elements have to manifest their transpositional activity in germ cells or their progenitors. The control of sexual development and function can be very versatile, and several studies have demonstrated the implication of transposable elements in the evolution of sex. In this review, we report the functional and evolutionary relationships between transposable elements and sexual reproduction in animals. In particular, we highlight how transposable elements can influence expression of sexual development genes, and how, reciprocally, they are tightly controlled in gonads. We also review how transposable elements contribute to the organization, expression and evolution of sexual development genes and sex chromosomes. This underscores the intricate co-evolution between host functions and transposable elements, which regularly shift from a parasitic to a domesticated status useful to the host.

Pea Ty1-copia group retrotransposons: transpositional activity and use as markers to study genetic diversity in Pisum

2000 ◽  
Vol 263 (6) ◽  
pp. 898-907 ◽  
Author(s):  
S. R. Pearce ◽  
M. Knox ◽  
T. H. N. Ellis ◽  
A. J. Flavell ◽  
A. Kumar
Keyword(s):  
Genetic Diversity ◽  
Transpositional Activity
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