Study on the Formation of Iron Film on Plant Root Surface and Its Water Purification Effect in Electrolytic Coupled Biological Scaffold Constructed Wetland

2021 ◽  
Vol 11 (06) ◽  
pp. 1230-1237
Author(s):  
子琛 汪
Keyword(s):  
Constructed Wetland ◽  
Water Purification ◽  
Plant Root ◽  
Root Surface ◽  
Iron Film

Enhancement of the performance of constructed wetlands for wastewater treatment in winter: the effect of Tubifex tubifex

RSC Advances ◽  
2016 ◽  
Vol 6 (41) ◽  
pp. 34841-34848 ◽  
Author(s):  
Yan Kang ◽  
Jian Zhang ◽  
Huijun Xie ◽  
Zizhang Guo ◽  
Pengfei Li ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Constructed Wetlands ◽  
Constructed Wetland ◽  
Water Purification ◽  
Tubifex Tubifex ◽  
Batch Systems ◽  
Potential Use

An improved constructed wetland (CW) with the addition ofTubifex tubifexin winter was studied in laboratory batch systems. The outcomes of this study indicate that the potential use ofTubifex tubifexcould improve the ecosystem and water purification by CWs in winter.

scholarly journals Developing a method for real-time visualization of cellulase activity

2020 ◽  
Author(s):  
Pallavi Kumari ◽  
Tali Sayas ◽  
Patricia Bucki ◽  
Sigal Brown Miyara ◽  
Maya Kleiman
Keyword(s):  
Real Time ◽  
Plant Cell Wall ◽  
Plant Root ◽  
Cellulase Activity ◽  
Root Surface ◽  
Surface Microstructure ◽  
Root Extract ◽  
Cell Wall Degrading Enzymes ◽  
Real Time Visualization ◽  
Replication Technique

AbstractStudying the interactions between microorganisms and plant roots is crucial for understanding a variety of phenomena concerning crop yield and health. The role of root surface properties in these interactions, is rarely addressed. To this end, we previously built a synthetic system, from the inert polymer polydimethyl siloxane (PDMS), mimicking the root surface microstructure, using a replication technique. This replica enables the study of isolated effects of surface structure on microorganism-plant interactions. Since the root surface is composed mostly of cellulose, using cellulose-like materials as our replica, instead of PDMS, is the next logical step. This will enable following the hydrolysis of such surfaces as a result of microorganisms secreting Plant Cell Wall Degrading Enzymes (PCWDE), and in particular, cellulase. Visualization of such hydrolysis in a synthetic system can assist in studying the localization and activity of microorganisms and how they correlate with surface microtopography, separately from chemical plant signals.In this work, we modified the known carboxymethyl cellulase (CMC) hydrolysis visualization method to enable real-time tracking of cellulase activity of microorganisms on the surface. Surface was formed from pure CMC, rather than CMC incorporated in agar as is often done, and by that, eliminating diffusion issues. Acridine orange dye, which is compatible, at low concentrations, with microorganisms, as opposed to other routinely used dyes, was incorporated into the film. The dye disassociated from the film when hydrolysis occurred, forming a halo surrounding the point of hydrolysis. This enabled real-time visualization since the common need for post hydrolysis dyeing was negated. Using Root Knot Nematode (RKN) as a model organism that penetrates the plant root, we showed it was possible to follow microorganism cellulase secretion on the surface in the form of CMC film hydrolysis. Furthermore, the addition of natural additives, in the form of root extract was also shown to be an option and resulted in an increased RKN response. We tested our newly developed method by changing temperature and pH conditions and by characterization of the hydrolyzed surface using both Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM).This method will be implemented in the future on a root surface microstructure replica. We believe the combination of this new method with our previously developed root surface microstructure replication technique can open a new avenue of research in the field of plant root-microorganism interactions.

Solute Transport in the Soil near Root Surfaces

2000 ◽  
Author(s):  
Peter B. Tinker ◽  
Peter Nye
Keyword(s):  
Soil Solution ◽  
Dynamic Equilibrium ◽  
Pore Solution ◽  
Plant Root ◽  
Unit Length ◽  
Solution Concentration ◽  
Root Surface ◽  
Transport Processes ◽  
Soil Pores ◽  
Intact Root

We discussed in chapter 4 the movement of solute between small volumes of soil, and in chapter 5 some properties of plant roots and associated hairs, particularly the relation between the rate of uptake at the root surface and the concentration of solute in the ambient solution. In the chapters to follow, we consider the plant root in contact with the soil, and deal with their association in increasingly complex situations; first, when the root acts merely as a sink and, second, when it modifies its relations with the surrounding soil by changing its pH, excreting ions, stimulating microorganisms, or developing mycorrhizas. In this chapter, we take the simplest situation that can be studied in detail, namely, a single intact root alone in a volume of soil so large that it can be considered infinite. The essential transport processes occurring near the root surface are illustrated in figure 6.1. We have examined in chapter 3 the rapid dynamic equilibrium between solutes in the soil pore solution and those sorbed on the immediately adjacent solid surfaces. These sorbed solutes tend to buffer the soil solution against changes in concentration induced by root uptake. At the root surface, solutes are absorbed at a rate related to their concentration in the soil solution at the boundary (section 5.3.2); and the root demand coefficient, αa, is defined by the equation . . . I = 2παaCLa (6.1) . . . where I = inflow (rate of uptake per unit length), a = root radius, CLa = concentration in solution at the root surface. To calculate the inflow, we have to know CLa, and the main topic of this chapter is the relation between CLa, and the soil pore solution concentration CL. The root also absorbs water at its surface due to transpiration (chapter 2) so that the soil solution flows through the soil pores, thus carrying solutes to the root surface by mass flow (convection). Barber et al. (1962) calculated whether the nutrients in maize could be acquired solely by this process, by multiplying the composition of the soil solution by the amount of water the maize had transpired.

scholarly journals Hypothetical Protein Avin_16040 as the S-Layer Protein of Azotobacter vinelandii and Its Involvement in Plant Root Surface Attachment

2015 ◽  
Vol 81 (21) ◽  
pp. 7484-7495 ◽  
Author(s):  
Pauline Woan Ying Liew ◽  
Bor Chyan Jong ◽  
Nazalan Najimudin
Keyword(s):  
Cell Surface ◽  
Deletion Mutant ◽  
Azotobacter Vinelandii ◽  
Plant Root ◽  
Hypothetical Protein ◽  
Surface Hydrophobicity ◽  
Root Surface ◽  
Bacterial Cells ◽  
Wild Type ◽  
Content Type

ABSTRACTA proteomic analysis of a soil-dwelling, plant growth-promotingAzotobacter vinelandiistrain showed the presence of a protein encoded by the hypotheticalAvin_16040gene when the bacterial cells were attached to theOryza sativaroot surface. AnAvin_16040deletion mutant demonstrated reduced cellular adherence to the root surface, surface hydrophobicity, and biofilm formation compared to those of the wild type. By atomic force microscopy (AFM) analysis of the cell surface topography, the deletion mutant displayed a cell surface architectural pattern that was different from that of the wild type.Escherichia colitransformed with the wild-typeAvin_16040gene displayed on its cell surface organized motifs which looked like the S-layer monomers ofA. vinelandii. The recombinantE. colialso demonstrated enhanced adhesion to the root surface.

scholarly journals Design of Portable Hybrid Constructed Wetland Reactor for the Treatment of Wastewater

2021 ◽  
Vol 9 (VI) ◽  
pp. 53-58
Author(s):  
Swapnil Hiwrale
Keyword(s):  
Wastewater Treatment ◽  
Constructed Wetland ◽  
Water Purification ◽  
Water Solution ◽  
Water Shortage ◽  
Pilot Scale ◽  
General Term ◽  
Pollutant Removal ◽  
Treated Wastewater ◽  
The Way

The idea of the project is predicated on the methodology of preventing the wastewater and the way to form the water pure by using various techniques to form water purification. Nowadays the matter of water shortage increases especially we face the water problem in summer seasons only. we are designing and portable hybrid water solution for the treatment of wastewater during this study, pilot-scale hybrid constructed wetlands (CWs) and multistage horizontal subsurface flow CWs (HF CWs) are studied and compared for the treatment of raw urban wastewater. Additionally, the sand became clogged, while the mulch failed to. The effect of water height on the average pollutant removal wasn't determined but HF10 performed better regarding compliance with legal regulations. With this idea of the project, we could see how we will purify the wastewater and the way much we can see the index of the water purification. A survey of the magnitude of water-related stress at villages on the subject of a college campus or Maharashtra villages affects rural life. Especially in the summer seasons. Problems of availability, accessibility, and quality of Water In Maharashtra 17 districts are that which is laid low with water crises. Maharashtra had approved over R.s 7000 corer to complete the potable project in the scarcity hit area. In India 4% of water resources available from 18% water available in word. Wetland technology can provide cheap and effective wastewater treatment in both temperature and tropical climates and is suitable for adoption in both industrialized likewise as in developing nations this method is utilized for the removal of a range of pollutants and a broad verity of wastewater worldwide. it's one of the simplest methods to treated wastewater at source premises, effectively and economically. This general term accustomed describe different degrees of treatment, so as of skyrocketing treatment levels are preliminary, primary, secondary, and tertiary or advance wastewater treatment. Disinfection to get rid of pathogens sometimes follows the last treatment steps. After treatment of wastewater from constructed wetland reactor, 90 to 95% BOD is going to be satisfied than 85 to 90% Turbidity is going to be removed, and 70 to 80% Nutrient are going to be removed by phytoremediation method. Constructed wetland technology has played a vital role in achieving the changes in wastewater.

scholarly journals Root hair specification and its growth in response to nutrients

2021 ◽  
Vol 49 (2) ◽  
pp. 12258
Author(s):  
Xian HUANG ◽  
Tianzhi GONG ◽  
Mei LI ◽  
Cenghong HU ◽  
Dejian ZHANG ◽  
...  
Keyword(s):  
Root Hair ◽  
Reverse Genetics ◽  
Current Knowledge ◽  
Plant Root ◽  
Root Hairs ◽  
Root Surface ◽  
Root Surface Area ◽  
Root Hair Development ◽  
Hair Development ◽  
Calcium Iron

Plant root hairs are cylindrical tubular projections from root epidermal cells. They increase the root surface area, which is important for the acquisition of water and nutrients, microbe interactions, and plant anchorage. The root hair specification, the effect of root hairs on nutrient acquisition and the mechanisms of nutrients (calcium, iron, magnesium, nitrogen, phosphorus, and potassium) that affect root hair development and growth were reviewed. The gene regulatory network on root hair specification in the plant kingdom was highlighted. More work is needed to clone the genes of additional root hair mutants and elucidate their roles, as well as undertaking reverse genetics and mutant complementation studies to add to the current knowledge of the signaling networks, which are involved in root hair development and growth regulated by nutrients.

Sign in / Sign up

Export Citation Format

Share Document