Evaluation of Drought Tolerance in Peanut Varieties at the Germination Stage

Efforts for increasing rice production nationally, it is necessary to increase the productivity of a land. Utilization of marginal lands like drought land potential to increase rice production nationally. One effort to utilize marginal land like dry land is the use of high-yielding drought tolerant varieties. Until now high-yielding drought tolerant varieties still relatively rare. For this reason, it is necessary to create high-yielding drought tolerant varieties from plant breeding program. One of the standard programs in plant breeding to create high-yielding drought tolerant varieties is provision of genetic material as parental. Local varieties gogo rice Drought-tolerant potential to be used as parental in the plant breeding program to create of high-yielding drought tolerant rice varieties. For this reason, it is necessary to test drought tolerance of local rice cultivars. The study used 10 cultivars exploration results in East Aceh Regency namely Gameso, Sibengkok, Ramos Gunung, Sidol, Sigedul, Rias Kuning, Rias putih, Sibontok, Serumu, and Sileso. Drought tolerance testing using PEG solution (Polyethylene Glycol) 6000 156. 75 g / liter of H2O which is equivalent to the osmotic potential of -3 BAR and aquades as control (0 BAR) an germination stage and early vegetative stage. The results of the study show that 10 cultivars tested in the germination stage, 6 cultivars were selected as drought tolerant cultivars. Furthermore, 6 cultivars tested in the early vegetative stage showed the ability to recover at day 35 and then at day 42 showed no symptoms of drought. 6 drought tolerant cultivars are Gameso, Ramos Gunung, Sigedul, Rias Kuning, Sibontok, and Sileso.

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Identifikasi Toleransi Kekeringan Tetua Padi Hibrida pada Fase Perkecambahan Menggunakan Polietilen Glikol (PEG) 6000

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i3.13784 ◽

2017 ◽

Vol 44 (3) ◽

pp. 235

Author(s):

Yuni Widyastuti ◽

Bambang Sapta Purwoko ◽

Dan Muhamad Yunus

Keyword(s):

Drought Tolerance ◽

Hybrid Rice ◽

Block Design ◽

Seed Vigor ◽

Seminal Root ◽

Rice Varieties ◽

Peg 6000 ◽

Osmotic Solution ◽

Parental Lines ◽

Germination Stage

ABSTRACT Developing of drought tolerant hybrid rice varieties requires parental lines which have tolerance to drought. Polyethylene glycol (PEG) has been widely used as an osmotic solution for detecting drought tolerance at germination stage of rice. The objectives of this experiment were to evaluate variables of drought tolerance at germination stage and to select drought tolerance of parental lines using osmotic solution PEG 6000 at concentration of 25%. The experiment was conducted at the greenhouse ICABIOGRAD Cimanggu Bogor, during April-May 2014. The design of the experiment was arranged in factorial randomized block design with three replications. The first factor (genotype) had 21 levels and the second factor (osmotic potential) had two levels (0 and 25% concentration levels of PEG 6000). Based on the principal component analysis, six primary indicators were proposed for drought tolerance of rice at germination stage, i.e., germination percentage, seed vigor, seminal root length, seedling length and dry weight of seminal root. Based on the clustering analysis, at 73.76% of similarity rate, the 21 tested genotypes were divided into 2 groups. The genotypes which showed similar responses with Salumpikit were IR 58025B, GMJ 14B, IR 80154B, GMJ 15B, R 3, PK 90, and PK 12 respectively. Keywords: drought tolerance, germination, hybrid rice PEG, parental lines

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SELECTION TRAITS FOR CHILI PEPPER DROUGHT TOLERANCE AT GERMINATION STAGE USING POLYETHYLENE GLYCOL 6000 AND DIVERSITY AMONG 22 CHILI PEPPER GENOTYPES

Russian Journal of Agricultural and Socio-Economic Sciences ◽

10.18551/rjoas.2021-10.27 ◽

2021 ◽

Vol 118 (10) ◽

pp. 240-249

Author(s):

Z. Millah ◽

M. Syukur ◽

Sobir ◽

S.W. Ardie

Keyword(s):

Polyethylene Glycol ◽

Drought Tolerance ◽

Chili Pepper ◽

Polyethylene Glycol 6000 ◽

Germination Stage

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QTL mapping of drought tolerance at germination stage in wheat using the 50 K SNP array

Plant Genetic Resources ◽

10.1017/s1479262121000551 ◽

2021 ◽

pp. 1-8

Author(s):

Yi Ren ◽

Jindong Liu ◽

Jianxin Zhang ◽

Susanne Dreisigacker ◽

Xianchun Xia ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Treatment Time ◽

Recombinant Inbred Lines ◽

Effective Means ◽

Snp Array ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Germination Stage ◽

D Value

Abstract Drought is a major concern among abiotic stresses in wheat (Triticum aestivum L.) production. Breeding resistant cultivars are the most effective means to manage drought stress. F6 recombinant inbred lines (RIL) derived from the cross of Berkut/Worrakatta were used to identify quantitative trait loci (QTL) for drought tolerance at germination stage under treatment of PEG6000 using the wheat 50 K single nucleotide polymorphism (SNP) array. Twenty-eight linkage groups were constructed, covering a length of 2220.26 cM. Eighteen QTL were detected based on the drought tolerance coefficients and D-value, explaining 2.7–6.5% of the phenotypic variances, in which 15 were likely to be novel. Three QTL, QGR.xjau-5AS, QCL.xjau-5AS and QD.xjau-5AS for GR, CL and D-value, respectively, at physical positions of 11.70–20.61 Mb between markers AX-111258240 and AX-94458300 on chromosome 5AS accounted for 3.4–4.8% of the phenotypic variances. Three QTL, QGP.xjau-5DL, QSH.xjau-5DL and QD.xjau-5DL for GP, SH and D-value, respectively, were flanked by markers AX-94524442 and AX-110998507 at 560.42–567.39 Mb on chromosome 5DL, accounting for 4.4–6.5% of the phenotypic variances. In addition, the candidate genes TraesCS5A02G022100, TraesCS5B02G014200 and TraesCS5D02G563900 were predicted. Based on transcriptional expression data, the results showed that the expression level of TaGATAs-5A, TaUbox-5B and TaGSTP-5D changed with the increase of treatment time under drought stress in tolerant and sensitive varieties. These are interesting targets in mining drought tolerance genes and the improvement of drought tolerance in wheat.

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Effects of microscopic testa color and morphologyon the water uptake ability and drought tolerance of germination-stage rapeseed (Brassica napus L.)

Bioengineered ◽

10.1080/21655979.2021.2000789 ◽

2021 ◽

Vol 12 (2) ◽

pp. 9341-9355

Author(s):

Zong He Zhu ◽

Abdul Sami ◽

Zhi Peng Chen ◽

Maliha Fatima ◽

Wen Yin Zheng ◽

...

Keyword(s):

Brassica Napus ◽

Drought Tolerance ◽

Water Uptake ◽

Brassica Napus L ◽

Testa Color ◽

Germination Stage

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Identification of drought-tolerance genes in the germination stage of soybean

10.21203/rs.3.rs-112822/v1 ◽

2020 ◽

Author(s):

Xingzhen Zhao ◽

Zhangxiong Liu ◽

Huihui Li ◽

Yanjun Zhang ◽

Lili Yu ◽

...

Keyword(s):

Drought Stress ◽

Seed Germination ◽

Drought Tolerance ◽

Mixed Linear Model ◽

Snp Markers ◽

Soybean Plants ◽

Drought Tolerance Indices ◽

Stress Influences ◽

Tolerance Indices ◽

Germination Stage

Abstract Background Drought stress influences the vigor of plant seeds and inhibits seed germination, making it one of the primary environmental factors adversely affecting food security. The seed germination stage is critical to ensuring the growth and productivity of soybeans in soils prone to drought conditions. We here examined the genetic diversity and drought-tolerance phenotypes of 410 accessions of a germplasm diversity panel for soybean and conducted quantitative genetics analyses to identify loci associated with the drought-tolerance of seedlings. Results We uncovered significant differences among the diverse genotypes for 4 growth indices and 5 drought-tolerance indices, which revealed abundant variation among genotypes, upon drought stress, and for genotype × treatment effects. We also used 158,327 SNP markers, and performed GWAS for the drought-related traits. Our data met the conditions (PCA + K) for using a mixed linear model in TASSEL, and we thusly identified 26 SNPs associated with drought tolerance indices for germination stage soybean plants. These were distributed across 10 soybean chromosomes, and these explain 5.19–9.66% of the observed phenotypic variation. Nine SNP sites, including for example Gm20_34956219 and Gm20_34956219, were associated with two or more phenotypic indices, and there were nine SNP markers located in or adjacent to (within 500 kb) previously reported drought tolerance QTLs. These SNP led to our identification of 41 candidate genes related to drought tolerance in the germination stage. Conclusion The results of our study contribute to a deeper understanding of the genetic mechanisms underlying drought tolerance in soybeans at the germination stage, thereby providing a molecular basis for identifying useful soybean germplasm for breeding new drought-tolerant varieties.

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