scholarly journals Identifikasi Toleransi Kekeringan Tetua Padi Hibrida pada Fase Perkecambahan Menggunakan Polietilen Glikol (PEG) 6000

2017 ◽  
Vol 44 (3) ◽  
pp. 235
Author(s):  
Yuni Widyastuti ◽  
Bambang Sapta Purwoko ◽  
Dan Muhamad Yunus
Keyword(s):  
Drought Tolerance ◽  
Hybrid Rice ◽  
Block Design ◽  
Seed Vigor ◽  
Seminal Root ◽  
Rice Varieties ◽  
Peg 6000 ◽  
Osmotic Solution ◽  
Parental Lines ◽  
Germination Stage

ABSTRACT<br /><br />Developing of  drought tolerant hybrid rice varieties requires parental lines which have tolerance to drought. Polyethylene glycol (PEG) has been widely used as an osmotic solution for detecting drought tolerance at germination stage of rice. The objectives of this experiment were to evaluate variables of drought tolerance at germination stage  and to select drought tolerance of parental lines using osmotic solution PEG 6000 at concentration of 25%. The experiment was conducted at the greenhouse ICABIOGRAD Cimanggu Bogor, during April-May 2014. The design of the experiment was arranged in factorial randomized block design with three replications. The first factor (genotype) had 21 levels and the second factor (osmotic potential) had two levels (0 and 25% concentration levels of PEG 6000). Based on the principal component analysis, six primary indicators were proposed for drought tolerance of rice at germination stage, i.e., germination percentage, seed vigor, seminal root length, seedling length and dry weight of seminal root. Based on the clustering analysis, at 73.76% of similarity rate, the 21 tested genotypes were divided into 2 groups. The genotypes which showed similar responses with Salumpikit were IR 58025B, GMJ 14B, IR 80154B, GMJ 15B, R 3, PK 90, and PK 12 respectively. <br /><br />Keywords: drought tolerance, germination, hybrid rice PEG, parental lines <br /><br />

Combined Genetic Analysis of Brown Spot (Helminthosporium oryzae) Disease for Developed Hybrid Combinations and their Parental Lines in Hybrid Rice

2018 ◽  
Vol 1 ◽  
pp. 37-48
Author(s):  
Mostafa Mamdouh Elshenawy ◽  
Walid Hassan Elgamal ◽  
Galal Bakr Anis ◽  
Fatma Awad
Keyword(s):  
Hybrid Rice ◽  
Natural Infection ◽  
Block Design ◽  
Brown Spot ◽  
Male Sterile ◽  
Rice Varieties ◽  
Genetic Components ◽  
Female Line ◽  
Parental Lines ◽  
Helminthosporium Oryzae

This experiment was carried out at the Farm of Rice Research and Training Center, Sakha, Kafrelsheikh, Egypt during three rice grown seasons from 2014-2016. Three cytoplasmic male sterile lines, two wild abortive type (WA); IR69625A, IR70368A and one (K-type) K17A as females were tested with seven rice genotypes Giza 178, Giza 179, Giza 181,Giza 182, Sakha 105, GZ 6296-12-1-2-1-1 and HR195R as testers using line x tester model of hybridization. The parental lines and their resulting (21F1crosses) were evaluated in Randomized Complete Block Design (RCBD) experiment having three replications during 2015 and 2016 growing seasons and surrounded by susceptible rice varieties i.e. Giza 171, Giza 177 and Giza 159 as natural infection to brown spot (Helminthosporium oryzae). Three brown spot acceptability traits; disease severity (%), infected leaves (%) and infected grains/panicle (%) were observed during this investigation. The combined data was calculated over both seasons to test the interaction of the different genetic components. According to the results, the male parents Giza178, Giza179, HR195, Giza 181 and Giza 182 could be utilized either for producing new hybrid combinations or for developing new parental lines in brown spot resistance program. The female line K17A was found to have a good combiner to brown spot resistance. Evaluation of hybrids for heterosis breeding based on mean performance, better-parent (BP %) and mid-parent heterosis (MP %) indicated that, out of 21F1hybrid rice combinations, one hybrid K17A x Giza182 found significantly desirable values for most brown spot traits and can be utilized as commercial hybrids resistant to brown spot disease.

scholarly journals Pengaruh pemupukan N terhadap serapan dan efisiensi penggunaan N, serta hasil padi hibrida

Jurnal Agro ◽  
2022 ◽  
Vol 8 (2) ◽  
pp. 262-273
Author(s):  
Risqa Naila Khusna Syarifah ◽  
Zulfa Ulinuha ◽  
Purwanto Purwanto
Keyword(s):  
Hybrid Rice ◽  
Block Design ◽  
N Uptake ◽  
N Use Efficiency ◽  
Optimum Dose ◽  
Rice Varieties ◽  
Randomized Block Design ◽  
Use Efficiency ◽  
N Use

Pemupukan N pada padi hibrida menjadi krusial mengingat varietas padi hibrida sangat responsif, sehingga harus diketahui dosis yang tepat untuk menghasilkan produksi yang tinggi. Penelitian ini bertujuan untuk mengkaji pengaruh dosis N terhadap serapan N, efisiensi penggunaan N, dan hasil padi hibrida. Penelitian menggunakan Rancangan Acak Kelompok yang diulang tiga kali. Faktor pertama adalah varietas padi hibrida yang terdiri dari Varietas Mapan P05, Varietas SL-8 SHS Sterling, dan Varietas Intani 602. Faktor kedua adalah dosis pemupukan N yang terdiri dari kontrol tanpa pemupukan N, dosis N 100 kg ha-1, dan dosis N 200 kg ha-1. Terdapat respon yang beragam antar varietas padi hibrida terhadap taraf pemupukan N, Serapan N, efisiensi penggunaan N tertinggi yang dihasilkan oleh varietas Intani 602 masing-masing sebesar 138,57 %, dan 36,13%. Serapan N tanaman padi tertinggi dicapai pada dosis N 100 kg ha-1, dan efisiensi penggunaan N tertinggi pada dosis N 200 kg ha-1. Hasil gabah tertinggi dicapai pada varietas Mapan P05 sebesar 7,42 t ha-1, dan dosis pemupukan N 100 kg ha-1 memberikan hasil tertinggi sebesar 7,47 t ha-1. Implikasi dari penelitian ini bahwa dosis nitrogen 100 kg ha-1 dapat menjadi acuan sebagai dosis pemupukan N varietas padi hibrida di Indonesia. Hybrid rice is responsive to nitrogen, so it’s necessary to find the optimum dose to optimize the production. The  aim of this research was to examine the effect of nitrogen on N uptake, N use efficiency, and yield of hybrid rice. This study used a randomized block design with three replications. The first factor consisted of the  Mapan P05 variety, the SL-8 SHS Sterling variety, and Intani 602 variety. The second factor was Nitrogen dosage consisted of control, 100 kg ha-1, and 200 kg ha-1. There were various responses among hybrid rice varieties to the level of fertilization. The highest N uptake and N use efficiency was achieved in the Intani 602 variety at 138.57% and 36.13%, respectively. The highest N uptake was achieved at 100 kg ha-1 of N, and the highest N use efficiency was at 200 kg ha-1. The highest yield was achieved in the Mapan P05 variety (7.42 t ha-1), and the dose of N at 100 kg ha-1 gave the highest yield (7.47 t ha-1). The implication of this research is that the nitrogen dose of 100 kg ha-1 can be used as a reference for hybrid rice varieties fertilizer in Indonesia.

scholarly journals Penentuan Indeks Seleksi Toleransi Kekeringan Galur Dihaploid Padi Sawah Tadah Hujan pada Fase Perkecambahan

2018 ◽  
Vol 46 (2) ◽  
pp. 133 ◽  
Author(s):  
Miftahur Rizqi Akbar ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Willy Bayuardi Suwarno
Keyword(s):  
Root Length ◽  
Block Design ◽  
Principal Component ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seminal Root ◽  
Peg 6000 ◽  
Rainfed Rice ◽  
Index Matching ◽  
Seminal Roots

ABSTRACT<br /><br />Rainfed rice tolerant to drought and high yielding would be an alternative to rainfed lowland areas prone to drought stress. Selection in the early phase of plant growth will accelerate the effort to obtain rainfed varieties. The objective of this experiment was to identify characters that significantly affect the selection of drought tolerance using PEG 6000 concentration of 25% in the germination phase. The experiment was conducted in greenhouse of ICABIOGRAD, Bogor, using a factorial randomized complete block design with three replications. The first factor was 32 rice genotypes while the second factor was 2 levels of PEG 6000, i.e., concentrations of 0 and 25%. The results showed that the correlation analysis and principal component analysis obtain the important characters namely the germination percentage, seminal root length, and the dry weight of seminal roots. Based on discriminant analysis, the index matching value of 96.77% was suitable to distinguish tolerant and sensitive genotypes using PEG 6000 concentration of 25% in the germination phase.<br /><br />Keywords: germination percentage, seminal root length, root dry weight<br /><br />

scholarly journals IDENTIFICATION OF WIDE COMPATIBILITY VARIETIES IN SOME TROPICAL JAPONICA RICE

2013 ◽  
Vol 6 (1) ◽  
pp. 28 ◽  
Author(s):  
A. Hairmansis ◽  
Hajrial Aswidinnoor ◽  
Trikoesoemaningtyas Trikoesoemaningtyas ◽  
Suwarno Suwarno
Keyword(s):  
Hybrid Rice ◽  
Block Design ◽  
Hybrid Sterility ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Major Barrier ◽  
F1 Hybrid ◽  
Tropical Japonica ◽  
Wide Compatibility ◽  
Randomized Complete Block Design

The F1 hybrid sterility in indica/japonica crosses is the major barrier in developing hybrid rice varieties between these two diverse germplasm. The sterility problem in japonica/indica hybrids can be overcome by using wide compatibility genes. The objective of this study was to identify wide compatibility varieties (WCVs) in some tropical japonica rice. Twenty five tropical japonica varieties as male parents were crossed with indica (IR64) and japonica (Akitakomachi) testers as female parents. The crosses were planted following a randomized complete block design with three replications. Varieties having average spikelet fertility of more than 70% with both the indica and japonica testers were rated as WCVs. Result from this study showed that six tropical japonica varieties were classified as WCVs, i.e., Cabacu, Grogol, Kencana Bali, Klemas, Lampung Lawer, and Napa. Hybrid sterility is caused by partial sterility of male and female gametes. The WCVs from the present study can be used in hybrid rice breeding program to solve hybrid sterility in indica/japonica hybrids.

scholarly journals Combining Ability for Drought Tolerance in Upland Rice Varieties at Reproductive Stage

2017 ◽  
Vol 9 (3) ◽  
pp. 138 ◽  
Author(s):  
G. M. Malemba ◽  
F. M. Nzuve ◽  
J. M. Kimani ◽  
M. F. Olubayo ◽  
J. W. Muthomi
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Combining Ability ◽  
Upland Rice ◽  
Block Design ◽  
Field Evaluation ◽  
Rice Production ◽  
Rice Varieties ◽  
F2 Population ◽  
Drought Conditions

Rice is an important food crop for human population ranking second among the mostly consumed cereal grains worldwide. Upland rice production is greatly constrained by drought stress resulting from rainfall variation patterns. Cultivation of drought tolerant varieties is considered the best option for drought management in rice production. The already released upland rice varieties are drought susceptible and have poor grain attributes hence, the aim of this study was to determine the combining ability for drought tolerance in upland rice. Four upland NERICA and two upland rice varieties were selected as parents for generating F1s crosses following 6 × 6 complete diallel. The generated 30 F1 crosses were advanced to F2 population for field evaluation. The F2 progenies together with six parents were planted in two sites; KALRO-Mwea Center Farm and Kirogo research Farm following a randomized complete block design in three replications. Drought stress was initiated 45 days after sowing after which data was collected on drought and agronomic parameters. The study revealed large genetic variations among the genotypes used. Both GCA and SCA were significant indicating the importance of both additive and non additive gene action in the expression of studied traits. In this study NERICA 2 and NERICA 15 were identified as good combiners for drought tolerance and grain yield under drought conditions. The single crosses namely; NERICA 15 × NERICA 2, NERICA 1 × NERICA 15, NERICA 11 × NERICA 15 and NERICA 2 × NERICA 15 were identified as superior for improving yield under drought conditions.

Evaluation of the tolerance of Thai indigenous upland rice germplasm to early drought stress using multiple selection criteria

2015 ◽  
Vol 15 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Kittichai Narenoot ◽  
Tidarat Monkham ◽  
Sompong Chankaew ◽  
Patcharin Songsri ◽  
Wattana Pattanagul ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Selection Criteria ◽  
Upland Rice ◽  
Block Design ◽  
Rice Variety ◽  
Tolerance Index ◽  
Rice Varieties ◽  
Rice Germplasm ◽  
Drought Tolerance Index

Drought remains the most important factor that affects rice productivity, especially in rainfed areas, worldwide. Upland rice is one of the crop choices of farmers in the rainfed environment. Although upland rice varieties require less water than lowland rice varieties, yields often remain limited by drought, particularly in the period of early growth. The aims of this study were to identify the traits related to early drought tolerance in upland rice varieties, and to identify the potential sources of germplasm for early drought tolerance. A total of sixty upland rice varieties were planted in a factorial experiment with a randomized complete block design with 3 replications in the rainy seasons of 2011 and 2012, under greenhouse conditions. Based on the drought tolerance index (DTI), the test germplasm sources were classified into three groups: (i) susceptible; (ii) moderately tolerant; (iii) tolerant to drought stress. Grain yield (GY) showed significant negative correlations with the leaf rolling score (r= − 0.623, P< 0.01), the leaf death score (LDS) (r= − 0.673, P< 0.01) and the recovery score (r= − 0.746, P< 0.01), while leaf dry matter (r= 0.698, P< 0.01) and leaf water potential (r= 0.618, P< 0.01) had significant positive correlations with GY. These findings indicate the suitability of the DTI as the selection criteria for early drought tolerance in a breeding programme. In addition, the upland rice germplasm accessions KKU-ULR011, KKU-ULR012, KKU-ULR125, KKU-ULR199 and KKU-ULR292 were identified as having high levels of stability for drought tolerance in both the 2011 and 2012 experiments, suggesting their potential for further use for rice variety improvement for drought tolerance.

scholarly journals Screening Gogo Rice Local Aceh Cultivars Drought Tolerant Under Polyetilene Glycol 6000

2019 ◽  
Vol 6 (2) ◽  
pp. 200-210
Author(s):  
Muhammad Syahril ◽  
Syamsul Bahri ◽  
Rhido Suhada
Keyword(s):  
Drought Tolerance ◽  
Plant Breeding ◽  
Genetic Material ◽  
Rice Production ◽  
Breeding Program ◽  
Vegetative Stage ◽  
Rice Varieties ◽  
Drought Tolerant ◽  
Plant Breeding Program ◽  
Germination Stage

Efforts for increasing rice production nationally, it is necessary to increase the productivity of a land. Utilization of marginal lands like drought land potential to increase rice production nationally. One effort to utilize marginal land like dry land is the use of high-yielding drought tolerant varieties. Until now high-yielding drought tolerant varieties still relatively rare. For this reason, it is necessary to create high-yielding drought tolerant varieties from plant breeding program. One of the standard programs in plant breeding to create high-yielding drought tolerant varieties is provision of genetic material as parental. Local varieties gogo rice Drought-tolerant potential to be used as parental in the plant breeding program to create of high-yielding drought tolerant rice varieties. For this reason, it is necessary to test drought tolerance of local rice cultivars. The study used 10 cultivars exploration results in East Aceh Regency namely Gameso, Sibengkok, Ramos Gunung, Sidol, Sigedul, Rias Kuning, Rias putih, Sibontok, Serumu, and Sileso. Drought tolerance testing using PEG solution (Polyethylene Glycol) 6000 156. 75 g / liter of H2O which is equivalent to the osmotic potential of -3 BAR and aquades as control (0 BAR) an germination stage and early vegetative stage. The results of the study show that 10 cultivars tested in the germination stage, 6 cultivars were selected as drought tolerant cultivars. Furthermore, 6 cultivars tested in the early vegetative stage showed the ability to recover at day 35 and then at day 42 showed no symptoms of drought. 6 drought tolerant cultivars are Gameso, Ramos Gunung, Sigedul, Rias Kuning, Sibontok, and Sileso.

scholarly journals Kendali Genetik Toleransi Kekeringan pada Padi Sawah (Oryza sativa L.)

2016 ◽  
Vol 44 (2) ◽  
pp. 119 ◽  
Author(s):  
Punjung Medaraji Suwarno ◽  
Desta Wirnas ◽  
Dan Ahmad Junaedi
Keyword(s):  
Drought Tolerance ◽  
Oryza Sativa L ◽  
Block Design ◽  
Irrigation Treatment ◽  
Tiller Number ◽  
Wet Season ◽  
Rice Varieties ◽  
Narrow Sense Heritability ◽  
Additive Variance ◽  
Rainfed Rice

ABSTRACT<br /><br />Drought has become a main barrier of rainfed rice in dry climate and short wet season areas. Growing drought-tolerance varieties is a practical approach to minimize yield lost of rice by the drought stress condition. The aims of this research were to examine the inheritance of drought tolerance character, to identify selection traits, and to estimate genetic gain for drought tolerant on lowland rice. Four rice varieties, i.e., Jatiluhur, Mentik Wangi, IR64, Way Apo Buru, were fully diallel crossed. The parent varieties and the F1’s were grown at Sawah Baru, Dramaga experimental field in two irrigation treatments, optimum and sub optimum where irrigation was applied until three weeks after transplanting. A randomized complete block design with three replications for each irrigation treatment was applied to the experiment. The results showed that there is a significant maternal effect on tiller number. The values of additive variance were higher than the dominance for all other characters except tiller number and grain weight per plant. The highest value for narrow sense heritability was achieved by panicle length.<br /><br />Keywords: diallel, drought, genetic, rice<br /><br />

scholarly journals Root Distribution and Its Impacts on the Drought Tolerance Capacity of Hybrid Rice in the Sichuan Basin Area of China

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 79 ◽  
Author(s):  
Xuechun Wang ◽  
Naseem Samo ◽  
Lamei Li ◽  
Mengran Wang ◽  
Muslim Qadir ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Root System ◽  
Root Distribution ◽  
Hybrid Rice ◽  
Soil Layer ◽  
Tolerance Index ◽  
Deep Soil ◽  
Rice Varieties ◽  
Major Factors

Drought is one of the major factors limiting rice yield worldwide. A total of 46 hybrid rice varieties were chosen to investigate their root distribution and their response to drought. A field experiment was carried out in a dry shed building to evaluate the drought tolerance capacity of hybrid rice varieties on the basis of CTIRDE (complex tolerance index of rice under drought environment) values. Next, the experiment was conducted in a specially designed pot system and seed bags to analyze the root distribution and activity of antioxidant enzymes in different rice varieties. Moreover, the DEEPER ROOTING 1 (DRO1) gene was sequenced to elucidate its role in the root distribution of typical rice varieties. On the basis of CTIRDE values, the 46 hybrid rice varieties were classified as tolerant (CTIRDE ≥ 0.75), semi-tolerant (0.75 > CTIRDE > 0.65), or sensitive (CTIRDE ≤ 0.65) to drought stress. The tolerant varieties (Chuanguyou208 and Deyou4727) displayed a significantly larger length, had higher number and weight of roots in the 30–50 cm soil layer, and exhibited a significantly higher activity of Superoxide dismutase (SOD) and Peroxidase (POD) enzymes in roots during the drought stress period. The DRO1 gene sequencing results revealed that the tolerant and sensitive varieties exhibited a single-nucleotide polymorphism (SNP) in the 3-exon region, and the tolerant varieties (Chuanguyou208 and Deyou4727) exhibited a larger root growth angle with the horizontal axis, hence developing a deeper root system as compared with the other two group varieties. A significant correlation was found not only between the DRO1 gene and root distribution but also between DRO1 and the activity of SOD and POD enzymes. Conclusively, as a key feature, a deep root system enabled tolerant rice varieties (Chuanguyou208 and Deyou4727) to avoid drought stress by absorbing more water stored in deep soil layers. The root distribution, activity of POD and SOD enzymes in roots, and DRO1 gene can be used to screen tolerant rice varieties that can survive better under drought stress during the seedling stage of rice growth.

scholarly journals Drought tolerance selection of GT1 rubber seedlings with the addition of polyethylene glycol (PEG) 6000

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
SYARIFAH AINI PASARIBU ◽  
Mohammad Basyuni ◽  
EDISON PURBA ◽  
YAYA HASANAH
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Morphological Characters ◽  
Sensitivity Index ◽  
Shoot Height ◽  
Peg 6000 ◽  
Rate Of Increase ◽  
Osmotic Solution

Abstract. Pasaribu SA, Basyuni M, Purba E, Hasanah Y. 2021. Drought tolerance selection of GT1 rubber seedlings with the addition of polyethylene glycol (PEG) 6000. Biodiversitas 22: 394-400. Tolerance of the rootstock of rubber to drought stress is not optimal. The root system can therefore be used as an indicator of drought tolerance to inhibit the growth of the plant. A good root system of rubber rootstocks is thought to help the plants acquire greater resistance to drought stress. Polyethylene glycol 6000 osmotic solution controls the water potential in the growing medium. This study aimed to analyze the effect of PEG 6000 osmotic solution (0%; 7.5%; and 15% concentration) on rubber seedlings leading to the development of morphological characters and sensitivity index. GT1 (Gondang Tapen 1) rubber seeds to be used as planting material were collected from the seed source garden of PT Socfin Indonesia. The study was carried out from January to February 2019, using a non-factorial randomized block design. The morphological characters observed were tapped root length, shoot height, the increased rate of taproot length, shoot height increase rate, ratio of taproot length, and shoot height. Data were analyzed using analysis of variance, discriminant, and drought stress sensitivity index. The results showed that the addition of PEG 6000 in planting media in vitro significantly influenced the character of the rate of increase of taproot length. The sensitivity index of taproot increase rate and the ratio of taproot length and shoot height was moderate at 7.5% levely contrast, the addition of PEG 6000 in planting media in vitro did not significantly impact it. The present study suggested that the rate of increase of taproot length was a distinguishing character showing the initial tolerance level of the GT1 seedlings to drought.

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