scholarly journals Rickettsia-like organisms (R.L.O.) infections of fin-fish

2017 ◽  
Vol 55 (2) ◽  
pp. 165 ◽  
Author(s):  
F. ATHANASSOPOULOU (Φ. ΑΘΑΝΑΣΟΠΟΥΛΟΥ) ◽  
E. KARAGOUNI (ΚΑΡΑΓΚΟΥΝΗ Ε.)
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Olfactory Nerve ◽  
Internal Organs ◽  
European Sea Bass ◽  
Salmonid Species ◽  
Diseased Fish ◽  
The Brain ◽  
Mass Mortalities ◽  
Of Greece

Rickettsia-like organisms (R.L.O.) infections of finfish have been reported in several salmonid and non-salmonid species in both fresh and seawater since 1939. This organism was not considered of economical importance to the global fin-fish aquaculture industry until Piscirickettsia salmonis was confirmed as the etiology agent of mass mortalities in the Chile during the 1990's. All cultured salmonid species can be infected by this intracellular bacteria and in diseased fish it may provoke a systemic response affecting most internal organs, but preferentially targeting the liver.For other R.L.O's the pathology may vary depending on both the imunogenicity of the R.L.O. and the species offish affected; eg, R.L.O. infections of the Hawaiian tilapia result in a systemic granulomatous inflammatory response. Initial published reports on R.L.O's affecting cultured juvenile sea bass (Dicentrarchus labrax) were described from sea-cages, at rearing temperatures ranging from 12-15°C, in the Mediterranean off the coast of France. In this outbreak the reported pathology was restricted to the mesencephalic regions of the brain. Subsequendy, the organism was identified from cultured sea bass of the coast of Greece, with moribund fish showing similar pathology; i.e brain, olfactory nerve and internal organs inflammation. These samples were preliminarily screened by immunohistochemistry and found to cross react with antisera to P. salmonis. This finding was also confirmed by demonstrating antigenic similarities between P. salmonis and European sea bass R.L.O. isolates from Greece. Recendy, a systemic type of the disease was demonstrated by histopathology in juvenile cultured juvenile sea bass. The meningitis, as well as other internal lesions affecting sea bass, is nearly identical to that seen in for R.L.O. infected Adantic salmon from British Columbia, Canada. Similar lesions have been noted for piscirickettsiosis in both Chilean and Eastern Canadian outbreaks: i.e liver, pancreas, retina, brain stem, meninges and the lamina propria of intestine.

scholarly journals Melatonin Binding Sites in the Brain of European Sea Bass (Dicentrarchus labrax)

2004 ◽  
Vol 21 (4) ◽  
pp. 427-434 ◽  
Author(s):  
María José Bayarri ◽  
Rosa Garcia-Allegue ◽  
JoséAntonio Muñoz-Cueto ◽  
Juan Antonio Madrid ◽  
Mitsuo Tabata ◽  
...  
Keyword(s):  
Binding Sites ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass ◽  
The Brain

scholarly journals Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax

2013 ◽  
Vol 14 (4) ◽  
pp. 7603-7616 ◽  
Author(s):  
Arianna Servili ◽  
Patricia Herrera-Pérez ◽  
María del Carmen Rendón ◽  
José Muñoz-Cueto
Keyword(s):  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Gnrh Receptor ◽  
Receptor Expression ◽  
European Sea Bass ◽  
The Brain

scholarly journals Genome-wide epigenomic stress response in the European sea bass (Dicentrarchus labrax, L.)

2020 ◽  
Author(s):  
Madoka V. KRICK ◽  
Erick DESMARAIS ◽  
Athanasios SAMARAS ◽  
Elise GUERET ◽  
Arkadios DIMITROGLOU ◽  
...  
Keyword(s):  
Stress Response ◽  
Cytosine Methylation ◽  
Acute Stress ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Blood Parameter ◽  
European Sea Bass ◽  
Genome Wide ◽  
Stress Related Genes ◽  
The Brain

Abstract Background: While the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.Results: We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Minor changes in RBC methylome appear to manifest after the stress challenge. Only, fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. However, literature surveys indicated that 38 of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, appear especially relevant to integrate a centrally produced stress response.Conclusion: By putting forward DMCs associated to stress-related genes, we show that minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress and components of the stress response without sacrificing fish. In parallel to blood parameter measurements (e.g. cortisol, glucose levels), and other molecular approaches (e.g. gene expression variation), features of the epigenetic landscape may offer new opportunities for biomonitoring components of the stress response in fish.

scholarly journals Genome-Wide Epigenomic Stress Response in the European Sea Bass (Dicentrarchus Labrax, L.)

2020 ◽  
Author(s):  
Madoka V. KRICK ◽  
Erick DESMARAIS ◽  
Athanasios SAMARAS ◽  
Elise GUERET ◽  
Arkadios DIMITROGLOU ◽  
...  
Keyword(s):  
Stress Response ◽  
Cytosine Methylation ◽  
Acute Stress ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass ◽  
Nucleated Red Blood Cells ◽  
Genome Wide ◽  
A Genome ◽  
The Brain

Abstract Background: While the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.Results: We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. Literature surveys indicated that thirty-eight of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, are especially relevant.Conclusion: We provide an improved epiGBS protocol with increased multiplexing and sequencing capacities that offer new opportunities to improve data acquisition and to investigate important biological processes at a genome-wide level, such as the stress response. Minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress without sacrificing fish.

scholarly journals Genome-wide epigenomic stress response in the European sea bass (Dicentrarchus labrax, L.)

2020 ◽  
Author(s):  
M.V. Krick ◽  
E. Desmarais ◽  
A. Samaras ◽  
E. Gueret ◽  
A. Dimitroglou ◽  
...  
Keyword(s):  
Stress Response ◽  
Cytosine Methylation ◽  
Acute Stress ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass ◽  
Nucleated Red Blood Cells ◽  
Genome Wide ◽  
A Genome ◽  
The Brain

AbstractBackgroundWhile the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.ResultsWe retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. Literature surveys indicated that thirty-eight of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, are especially relevant.ConclusionWe provide an improved epiGBS protocol with increased multiplexing and sequencing capacities that offer new opportunities to improve data acquisition and to investigate important biological processes at a genome-wide level, such as the stress response. Minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress without sacrificing fish.

Sign in / Sign up

Export Citation Format

Share Document