scholarly journals Culture and sporulation of Paenibacillus larvae larvae (White) in not specific culture media

2018 ◽  
Vol 52 (4) ◽  
pp. 303
Author(s):  
A. FYLAKTOU (Α. ΦΥΛΑΚΤΟΥ) ◽  
B. LIAKOS (Β. ΛΙΑΚΟΣ) ◽  
N. ILIADIS (Ν. ΗΛΙΑΔΗΣ)

In this experimental study the culture possibility and the sporulation ability of Paenibacillus larvae larvae (White), in simple and compound not specific culture media, in aerobic and anaerobic conditions and in an atmosphere of 5-10% C02 in air, were investigated. The results indicated that P.l.larvae could be cultured in normal culture media, in aerobic or anaerobic conditions. However its growth is significantly delayed and only after 48-72 h incubation, its demonstration becomes evident. In specific culture media like blood agar and Brucella agar and atmosphere of 5-10% C02 in air, it can be developed faster, sporulated more and demonstrated easier. Development and sporulation of P.l.larvae was better in Brucella agar than in blood agar.

1980 ◽  
Vol 26 (4) ◽  
pp. 475-481 ◽  
Author(s):  
Greg W. Gorder ◽  
E. Paul Lichtenstein

Oxygen concentration and different carbon sources drastically altered parathion degradation in culture media inoculated with microorganisms from Wisconsin cranberry (Vaccinium macrocarpon Ait.) growing soils. These microorganisms also grew in basal salts media utilizing parathion as the sole carbon source. 14CO2 was produced only from [phenyl-14C]parathion, whereas [ethyl-14C]parathion-derived radiocarbon remained in the stale media of the soil-free cultures. Addition of 0.05% glucose to basal salts medium inhibited [phenyl-14C]parathion degradation, whereas the addition of 0.05% yeast extract to basal salts medium also inhibited microbiological degradation of the insecticide to 14CO2, but to a lesser extent. Aminoparathion and aminoparaoxon were formed only in basal salts medium with 0.05% yeast extract. Aerobic cultures produced more 14CO2 and less aminoparathion from [phenyl-14C]parathion than did anaerobic cultures. Aminoparathion was more abundant in cultures with inocula obtained from the 18- to 23-cm soil layer than with culture inocula obtained from the 0- to 5-cm soil layer under both aerobic and anaerobic conditions.


2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.


1946 ◽  
Vol 24f (1) ◽  
pp. 1-11 ◽  
Author(s):  
G. A. Adams

Aeration by mechanical agitation of 15% wheat mash fermented by Aerobacillus polymyxa inhibited the formation of 2,3-butanediol and particularly of ethanol. Aeration of similar mashes by passage of finely dispersed air or oxygen at the rate of 333 ml. per minute per litre of mash increased the rate of formation and yield of 2,3-butanediol but inhibited ethanol formation. However, the over-all time required for the completion of fermentation was not shortened from the usual 72 to 96 hr. required for unaerated mashes. There was no evidence of a shift from fermentative to oxidative dissimilation. Under aerobic conditions, the final butanediol–ethanol ratio was approximately 3:1. Anaerobic conditions, as produced by the passage of nitrogen or hydrogen through the mash, increased the rate of formation of both butanediol and ethanol and shortened the fermentation time to about 48 hr. Under these conditions, the butanediol–ethanol ratio was reduced to about 1.3:1.0. Carbon dioxide gave a butanediol–ethanol ratio resembling that of anaerobic fermentation but did not reduce fermentation time.


Author(s):  
Irina Alina Chera Anghel ◽  
Loredana Popescu

The most commonly used insulating liquid in transformers is mineral oil. Special synthetic applications such as silicone, ester, perchloroethene, etc. are used today in special applications, with different characteristics, very low or nonexistent toxicity to mineral oils used in transformers. On the other hand, they have a much better biodegradability than mineral oils in both aerobic and anaerobic conditions. But they cannot directly replace the mineral oil in operation or in repaired units. They have dielectric properties and good heat transfer but have limited their use to special transformers due to the relatively high cost and availability.


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