scholarly journals Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 966
Author(s):  
Amrullah Amrullah ◽  
wahidah wahidah ◽  
ardiansyah ardiansyah ◽  
indrayani indrayani
Keyword(s):  
Immune Response ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Challenge Test ◽  
Average Weight ◽  
Bacterial Diseases ◽  
Maternal Immunity ◽  
Polyvalent Vaccine ◽  
Alternative Means ◽  
Offspring Protection

Background: Vaccination is an effective and alternative means of disease prevention, however, it cannot be conducted on the offspring of fish.  For this process to take place, the transfer of maternal immunity must be implemented. This study aims to determine the effectiveness of transferring immunity from the broodstock to the offspring using a polyvalent vaccine against Aeromonas hydrophila, Streptococcus agalactiae, and Pseudomonas fluorescens in Nile tilapia, Oreochromis niloticus. Methods: Nile tilapia broodstock, with an average weight of 203g (±SD 23 g) was injected with a vaccine used as a treatment. Example include A. hydrophila monovalent (MA), S. agalactiae monovalent (MS), P. fluorescens monovalent (MP), A. hydrophila and S. agalactiae bivalent (BAS), A. hydrophila and P. fluorescens bivalent (BAP), P. fluorescens and S. agalactiae bivalent (BPS), and A. hydrophila, S. agalactiae, and P. fluorescens polyvalent vaccines (PAPS). While the control was fish that were injected with a PBS solution. The broodstock’s immune response was observed on the 7th, 14th, 21st, and 28th day, while the immune response and challenge test on the offspring was conducted on the 10th, 20th, 30th, and 40th day during the post-hatching period. Result: The application of PAPS in broodstock could significantly induce the best immune response and immunity to multiple diseases compared to other treatments. The RPS of the PAPS was also higher than the other types of vaccines. This showed that the transfer of immunity from the broodstock to the Nile tilapia offspring could protect it against bacterial diseases such as A. hydrophila, S. agalactiae, and P. fluorescens. Conclusion: The application of PAPS A. hydrophila, S. agalactiae, P. fluorescens vaccines increased the broodstock’s immune response and it was transferred to their offsprings. They were able to produce tilapia seeds that are immune to diseases caused by A. hydrophila, S. agalactiae, and P. fluorescens.

Transfer of maternal immunity project

2021 ◽  
Author(s):  
Amrullah
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Maternal Immunity ◽  
Polyvalent Vaccine ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Offspring Protection

This preprint is a part of the article "Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus"

scholarly journals Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Gercyana Marentek ◽  
Henky Manoppo ◽  
Sammy N.J Longdong
Keyword(s):  
Immune Response ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Allium Sativum ◽  
Control Fish ◽  
Total Leukocyte Count ◽  
Average Weight ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Nonspecific Immune Response ◽  
Phagocytosis Activity

The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth

Generation of mouse monoclonal antibodies specific to tilapia immunoglobulin using fish immunoglobulin/BSA complex for monitoring of the immune response in Nile tilapia Oreochromis niloticus

2018 ◽  
Vol 50 (1) ◽  
pp. 277-283 ◽  
Author(s):  
Tanapon Soonthonsrima ◽  
Pradit Wangman ◽  
Parin Chaivisuthangkura ◽  
Chalinan Pengsuk ◽  
Paisarn Sithigorngul ◽  
...  
Keyword(s):  
Immune Response ◽  
Monoclonal Antibodies ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus
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