scholarly journals The Establishment and Characterization of Cell Lines Stably Expressing Human Ku80 Tagged with Enhanced Green Fluorescent Protein

2004 ◽  
Vol 45 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Manabu KOIKE ◽  
Aki KOIKE
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 632
Author(s):  
Yingyun Cai ◽  
Shuiqing Yu ◽  
Ying Fang ◽  
Laura Bollinger ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Infectious Clone ◽  
Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Hemorrhagic Fever Virus ◽  
Green Fluorescent

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.

In Vitro and In Vivo Characterization of Recombinant Ebola Viruses Expressing Enhanced Green Fluorescent Protein

2007 ◽  
Vol 196 (s2) ◽  
pp. S313-S322 ◽  
Author(s):  
Hideki Ebihara ◽  
Steven Theriault ◽  
Gabriele Neumann ◽  
Judie B. Alimonti ◽  
Joan B. Geisbert ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent ◽  
In Vivo Characterization

Characterization of a recombinant encephalomyocarditis virus expressing the enhanced green fluorescent protein

2006 ◽  
Vol 151 (9) ◽  
pp. 1783-1796 ◽  
Author(s):  
S. Hammoumi ◽  
C. Cruciere ◽  
M. Guy ◽  
A. Boutrouille ◽  
S. Messiaen ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Encephalomyocarditis Virus ◽  
Green Fluorescent

scholarly journals LBP Proteins Modulate SF1-Independent Expression of P450scc in Human Placental JEG-3 Cells

2005 ◽  
Vol 19 (2) ◽  
pp. 409-420 ◽  
Author(s):  
Ningwu Huang ◽  
Walter L. Miller
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Transcriptional Repression ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Transient Transfection ◽  
Glutathione S Transferase ◽  
Negative Effects ◽  
Chain Cleavage ◽  
Green Fluorescent

Abstract The cholesterol side-chain cleavage enzyme, P450scc, initiates biosynthesis of all steroid hormones. Adrenal and gonadal P450scc expression requires steroidogenic factor-1 (SF1), but P450scc expression in human placental JEG-3 cells utilizes an SF1-independent element at −155/−131 that is inactive in adrenals and gonads. We previously cloned two transcription factors, long terminal repeat binding protein (LBP)-1b and LBP-9, from JEG-3 cells. In transient transfection assays, LBP-1b activated the −155/−131 element whereas LBP-9 suppressed its LBP-1b-stimulated expression. To assess the roles of these factors on the intact P450scc gene, we stably expressed LBP-1b or LBP-9 in JEG-3 cells. All cell lines stably expressing a fusion protein of LBP-1b and enhanced green fluorescent protein increased P450scc expression, but cell lines stably expressing LBP-9 fused to enhanced green fluorescent protein either increased or decreased P450scc expression. 8-Br-cAMP induced endogenous LBP-9, but not LBP-1b expression. Glutathione-S-transferase pull-down assays showed that LBP-1b and LBP-9 can dimerize with themselves and with each other; LBP-1b residues 300–540 and LBP-9 residues 300–479 were required for dimer formation. Glutathione-S-transferase pull-down assays, bandshifts, and transient transfection assays showed that TReP-132 (another factor that can bind to −155/−131) does not interact with either LBP-1b or LBP-9, or influence their ability to induce or suppress transcription from the −155/−131 element. Gal4 transactivation assays showed that transcriptional repression activity by LBP-9 requires residues 100–200. RNAi interference of either LBP-1b or LBP-9 mRNAs decreased P450scc expression. LBP-1b is an important SF1-independent transcriptional activator stimulating P450scc expression in human placental JEG-3 cells, whereas LBP-9 modulates the action of LBP-1b, exerting both positive and negative effects.

Generation and characterization of a trackable plant-made influenza H5 virus-like particle (VLP) containing enhanced green fluorescent protein (eGFP)

2015 ◽  
Vol 29 (9) ◽  
pp. 3817-3827 ◽  
Author(s):  
Katie R. Young ◽  
Guillaume Arthus-Cartier ◽  
Karen K. Yam ◽  
Pierre-Olivier Lavoie ◽  
Nathalie Landry ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Virus Like Particle ◽  
Green Fluorescent
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