scholarly journals Assessment of In-Vitro inhibitory effect of khaya tea infusion on porcine pancreatic lipase activity

2015 ◽  
Vol 6 (6) ◽  
pp. 1-9 ◽  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Inhibitory Effect ◽  
Porcine Pancreatic Lipase ◽  
Tea Infusion

scholarly journals Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity ofDiospyros kakiFruit andCitrus unshiuPeel

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Gyo-Nam Kim ◽  
Mi-Rae Shin ◽  
Sung Ho Shin ◽  
Ah Reum Lee ◽  
Joo Young Lee ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Dietary Fat ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Diospyros Kaki ◽  
Fat Absorption ◽  
Dietary Fat Absorption

Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme ofDiospyros kakifruit andCitrus unshiupeel mixture extract (PCM) was evaluatedin vitroand its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed micein vivo. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC50value of 507.01 μg/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (p<0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase.

UJI IN VITRO EKSTRAK AIR DAN ETANOL DARI BUAH ASAM GELUGUR, RIMPANG LENGKUAS, DAN KENCUR SEBAGAI INHIBITOR AKTIVITAS LIPASE PANKREAS

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Dyah Iswantini ◽  
Latifah K. Darusman ◽  
Ana Fitriyani
Keyword(s):  
Body Weight ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Reduce Body Weight ◽  
Ethanol Extracts

Asam gelugur fruits of Garcinia often used to reduce body weight. Lengkuas and kencur are traditional herbal that potential for antiobesity because they could reduce the level of phospholipids, triglycerides, and cholesterol. The aim of the research was to evaluate the potencies of these herbal as antiobesity by measurement of their water and ethanol extracts capabilities as in vitro inhibitor of pancreatic lipase activity. The water and ethanol extracts of asam gelugur fruits contained saponins and alkaliods, respectively. The water extract of lengkuas rhizomes contained alkaloids, flavonoids, saponins, and quinones, while the ethanol extract contained alkaloids, flavonoids, saponins, and steroids. The water extract of kencur rhizomes contained saponins and quinones, while the ethanolextract contained alkaloids, flavonoids, steroids, and quinones. The highest inhibitory effect of all extracts was obtained from the ethanol extract of asam gelugur fruits with value of 86.3% at 150 ppm. The highest inhibitory effect of lengkuas extracts was from the ethanol extract at 200 ppm (56.2%). The highestinhibitory effect of kencur was showed by the ethanol extract with the value 37.6% at 300 ppm. These values were higher than the inhibitory effect of the positive control (Xenical®) at 100 ppm (10.6%).

IN VITRO STUDIES OF SOME EDIBLE SPICES ON PANCREATIC LIPASE INHIBITORY ACTIVITY

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (02) ◽  
pp. 62-68
Author(s):  
S Mhatre ◽  
◽  
A. Bhagit ◽  
R. P Yadav
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Syzygium Aromaticum ◽  
High Potency ◽  
Good Source ◽  
Zanthoxylum Armatum ◽  
Methanolic Extracts ◽  
Cinnamomum Tamala

Pancreatic lipase inhibitory effect of some edible spices in light of percent inhibition, efficacy, reversibility/ irreversibility and effect of pH on inhibition is presented here. Lipase inhibitory activities of methanolic extracts of eighteen spices were evaluated. Extracts of Zanthoxylum armatum, Cinnamomum tamala, Syzygium aromaticum and Myristica fragrans were considered to be of high potency in synthetic substrate assay. Only Syzygium aromaticum showed high potency in natural substrate based lipase assay. Zanthoxylum armatum extract displayed lowest IC50 of 9.0 μg/mL. On dialysis, all extracts lost their lipase inhibitory activity indicating reversible nature of inhibition. pH significantly affected the performance of spice extracts during inhibition of pancreatic lipase. Most of the extracts lost their pancreatic lipase inhibitory activity at pH 3.0 with the exception of Brassica nigra and Cinnamomum tamala. Results showed spice are good source of pancreatic lipase inhibitor and its potential as drug for obesity can be explored by addressing various issues.

Inhibitory Effect of Persimmon Tannin on Pancreatic Lipase and the Underlying Mechanism in Vitro

2018 ◽  
Vol 66 (24) ◽  
pp. 6013-6021 ◽  
Author(s):  
Wei Zhu ◽  
Yangyang Jia ◽  
Jinming Peng ◽  
Chun-mei Li
Keyword(s):  
Pancreatic Lipase ◽  
Underlying Mechanism ◽  
Inhibitory Effect

scholarly journals Caffeic Acid Derivatives from Bacopa monniera Plants as Inhibitors of Pancreatic Lipase Activity and their Structural Requirements

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101
Author(s):  
Souichi Nakashima ◽  
Tomoe Ohta ◽  
Seikou Nakamura ◽  
Yoshimi Oda ◽  
Mari Koumoto ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Methanol Extract ◽  
Bacopa Monniera ◽  
Inhibitory Effects ◽  
Active Constituent ◽  
Structural Requirements ◽  
Phenylpropanoid Glycosides ◽  
First Time

The methanol extract of whole Bacopa monniera plants inhibited pancreatic lipase activity in vitro. From this extract we have reported the isolation of 11 triterpene glycosides and 5 phenylethanoid- and/or phenylpropanoid- glycosides. In this paper, we describe the effects of the methanol extract and/or its constituents on pancreatic lipase activity and the isolation of an active constituent, desrhamnosyl isoacteoside. In addition, the structural requirements for its inhibitory effects were examined. We also examined the effects on the elevation of plasma triglyceride (TG) levels in olive oil loaded mice. The major active constituents, desrhamnosyl isoacteoside and plantainoside B, reduced plasma TG levels in the mice. The inhibitory effects of B. monniera and its constituents on pancreatic lipase activity and plasma TG level are reported for the first time.

scholarly journals Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 844
Author(s):  
Razia Sultana ◽  
Adeola M. Alashi ◽  
Khaleda Islam ◽  
Md Saifullah ◽  
C. Emdad Haque ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Inhibitory Activity ◽  
The Other ◽  
Converting Enzyme ◽  
Inhibitory Activities ◽  
Ash Gourd ◽  
Better Than

The aim of the study was to determine the in vitro enzyme inhibition activities of aqueous polyphenolic extracts of nine popular Bangladeshi vegetables, namely ash gourd, bitter gourd, brinjal, Indian spinach, kangkong, okra, ridge gourd, snake gourd, and stem amaranth. Polyphenolic glycosides were the major compounds present in the extracts. Inhibition of α-amylase (up to 100% at 1 mg/mL) was stronger than α-glucosidase inhibition (up to 70.78% at 10 mg/mL). The Indian spinach extract was the strongest inhibitor of pancreatic lipase activity (IC50 = 276.77 µg/mL), which was significantly better than that of orlistat (381.16 µg/mL), a drug. Ash gourd (76.51%), brinjal (72.48%), and snake gourd (66.82%) extracts were the most effective inhibitors of angiotensin-converting enzyme (ACE), an enzyme whose excessive activities have been associated with hypertension. Brinjal also had a significantly higher renin-inhibitory activity than the other vegetable extracts. We conclude that the vegetable extracts may have the ability to reduce enzyme activities that have been associated with hyperglycemia, hyperlipidemia, and hypertension.

scholarly journals IN VITRO ANTI-OBESITY EFFECT OF MACROLICHENS HETERODERMIA LEUCOMELOS AND RAMALINA CELASTRI BY PANCREATIC LIPASE INHIBITORY ASSAY

2017 ◽  
Vol 9 (5) ◽  
pp. 137
Author(s):  
Rashmi Shivanna ◽  
Hengameh Parizadeh ◽  
Rajkumar H. Garampalli
Keyword(s):  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Methanol Extract ◽  
Well Being ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Protective Role ◽  
Treatment Of Obesity ◽  
Molecular Sequencing

Objective: Obesity is a chronic disorder caused by an imbalance between energy intake and expenditure in which excessive fat will be deposited in adipose tissue and poses a risk to the health and well-being of humans. Agents which inhibit pancreatic lipase play an important role in the treatment of obesity. The aim of this study was to assess the potential effect of macro lichens Heterodermia leucomelos (L.) Poelt a foliose lichen and Ramalina celastri (Sprengel) Krog and Swinscow a fruticose lichen in the treatment of obesity.Methods: In vitro anti-obesity inhibitory effect of macro lichens were evaluated by using chicken pancreatic lipase activity. Lipase was extracted from the chicken pancreas. Different concentrations from 5-25 mg/ml of methanol and ethyl acetate extracts of lichens Heterodermia leucomelos and Ramalina celastri was incubated with pancreas lipase.Results: With the increase in the concentration of extracts the higher inhibition of the enzyme was observed. Solvent methanol showed good activity compared to ethyl acetate. Percentage of inhibition ranged from 19.7-69.8 and 20.0-86.6 % in the methanol extract of Heterodermia leucomelos and Ramalina celastri respectively. Comparatively lichen Ramalina celastri in methanol extract showed maximum inhibition of 86.6 %, whereas ethyl acetate showed an inhibition of 63.0% at 25 mg/ml against enzyme lipase.Conclusion: In the present study, the inhibitory activity of lichen indicates its protective role in treating obesity. Molecular sequencing of this lichen helps in future to determine the various metabolic pathways that are responsible for the production of novel compounds.

scholarly journals Inhibitory Activity of Benzophenones from Anemarrhena asphodeloides on Pancreatic Lipase

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yang Hee Jo ◽  
Seon Beom Kim ◽  
Jong Hoon Ahn ◽  
Qing Liu ◽  
Bang Yeon Hwang ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Soluble Fraction ◽  
Significant Inhibition ◽  
Dietary Fats ◽  
Lipid Absorption ◽  
Vitro Assay ◽  
Key Enzyme ◽  
Anemarrhena Asphodeloides

Pancreatic lipase is a key enzyme for lipid absorption by hydrolysis of total dietary fats. Therefore, inhibition of pancreatic lipase is suggested to be an effective therapy in the regulation of obesity. The EtOAc-soluble fraction of Anemarrhena asphodeloides rhizomes significantly inhibited pancreatic lipase activity as assessed using porcine pancreatic lipase as an in vitro assay system. Further fractionation of the EtOAc-soluble fraction of A. asphodeloides led to the isolation of a new benzophenone glycoside, zimoside A (1), together with the eleven known compounds iriflophenone (2), 2,4′,6-trihydroxy-4-methoxybenzophenone (3), foliamangiferoside A (4), (2,3-dihydroxy-4-methoxyphenyl)(4-hydroxyphenyl)-methanone (5), 1,4,5,6,-tetrahydroxyxanthone (6), isosakuranetin (7), 4-hydroxybenzoic acid (8), 4-hydroxyacetophenone (9), vanillic acid (10), tyrosol (11) and 5-hydroxymethyl-2-furaldehyde (12). Among the isolated compounds, 3, 5 and 10 showed significant inhibition of pancreatic lipase activity.

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