Serpins can block different steps in the activation cascade of prophenoloxidase (proPO) system, and play an important role in immunity of insect. In this paper, Haemolymph was collected from the 4th molting, newly moulted 5th instar and day-3 fifth instar larval challenged by lipopolysaccharide (LPS) or Bacillus thuringiensis, respectively. The results revealed that the transcriptional level of Bmserpin-6 in different developmental stages showed a trend of rise first, then fall. Bmserpin-6 of the 4th molting larva expressed highest at 6h post-infection with LPS and 3h post-infection with Bacillus thuringiensis. Bmserpin-6 of newly moulted 5th instar larva expressed highest at 9h post-infection with LPS and 6h post-infection with Bacillus thuringiensis. Bmserpin-6 of day-3 fifth instar larva expressed highest at 9h post-infection with Bacillus thuringiensis. Bmserpin-6 was all highly induced and highly expressed in haemolymph of larval at different developmental stages. But The time to arrive the highest transcriptional level was different. This is inferred that the Serpin gene may play an important role in immunity of Bombyx mori.
Pyramiding and evaluation of both a foreign Bacillus thuringiensis and a Lysine-rich protein gene in the elite indica rice 9311