Construction of a high-density genetic map and identification of QTLs for cucumber mosaic virus resistance in pepper (Capsicum annuum L.) using specific length amplified fragment sequencing (SLAF-seq)

El Cucumber mosaic virus (CMV) ocasiona una de las enfermedades virales más importantes a nivel mundial en plantas silvestres y cultivadas. En México son pocos los estudios que se han abordado con este virus, y dada su amplia gama de hospedantes e impacto económico, es necesario contar con mayor información de su presencia y distribución en zonas de importancia agrícola como las del estado de Colima. En este trabajo, se reportan nuevos aislamientos del CMV identificados por RT-PCR, secuenciación de DNA y su análisis filogenético: CMV-Vin en vinca (Catharanthus roseus), CMV-Chi en chile jalapeño (Capsicum annuum) y CMV-Tom en tomate saladette (Solanum lycopersicum). Se confirmó, además, la presencia del CMV en melón cantaloupe (Cucumis melo) (CMV-Mel). Los aislamientos CMV-Vin, CMV-Chi y CMV-Mel agruparon en el subgrupo IB, mientras que CMV-Tom agrupó en el subgrupo IA de CMV. De estos aislamientos, sólo CMV-Vin evidenció la presencia de un RNA satélite (satRNA Vin) sin dominio necrogénico. Este es el primer reporte de la presencia del CMV en vinca, chile y tomate y de un RNA satélite en vinca en Colima, México.

Download Full-text

Construction of a high-density genetic map and mapping of QTLs for soybean (Glycine max) agronomic and seed quality traits by specific length amplified fragment sequencing

BMC Genomics ◽

10.1186/s12864-018-5035-9 ◽

2018 ◽

Vol 19 (1) ◽

Cited By ~ 10

Author(s):

Yanwei Zhang ◽

Wei Li ◽

Yanhui Lin ◽

Lifeng Zhang ◽

Caijie Wang ◽

...

Keyword(s):

Glycine Max ◽

Genetic Map ◽

Seed Quality ◽

High Density ◽

Quality Traits ◽

Specific Length ◽

Seed Quality Traits

Download Full-text

Identification of quantitative trait loci associated with resistance to cucumber mosaic virus in Capsicum annuum

Theoretical and Applied Genetics ◽

10.1007/s001220100581 ◽

2001 ◽

Vol 102 (8) ◽

pp. 1213-1220 ◽

Cited By ~ 59

Author(s):

A. Ben Chaim ◽

R. C. Grube ◽

M. Lapidot ◽

M. Jahn ◽

I. Paran

Keyword(s):

Quantitative Trait Loci ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Capsicum Annuum ◽

Quantitative Trait ◽

Trait Loci

Download Full-text

The Suppressor of Transgene RNA Silencing Encoded by Cucumber mosaic virus Interferes with Salicylic Acid-Mediated Virus Resistance

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2001.14.6.715 ◽

2001 ◽

Vol 14 (6) ◽

pp. 715-724 ◽

Cited By ~ 158

Author(s):

Liang-Hui Ji ◽

Shou-Wei Ding

Keyword(s):

Salicylic Acid ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Virus Resistance ◽

Rna Silencing ◽

Defense Mechanism ◽

Antiviral Defense ◽

Oxidase Gene ◽

Systemic Spread ◽

Systemic Infections

The Cucumber mosaic virus (CMV)-encoded 2b protein (Cmv2b) is a nuclear protein that suppresses transgene RNA silencing in Nicotiana benthamiana. Cmv2b is an important virulence determinant but nonessential for systemic spread in N. glutinosa, in contrast to its indispensable role for systemic infections in cucumber. Here, we report that Cmv2b became essential for systemic infections in older N. glutinosa plants or in young seedlings pre-treated with salicylic acid (SA). Expression of Cmv2b from the genome of either CMV or Tobacco mosaic virus significantly reduced the inhibitory effect of SA on virus accumulation in inoculated leaves and systemic leaves. A close correlation is demonstrated between Cmv2b expression and a reduced SA-dependent induction of the alternative oxidase gene, a component of the recently proposed SA-regulated antiviral defense. These results collectively reveal a novel activity of Cmv2b in the inhibition of SA-mediated virus resistance. We used a N. tabacum line expressing a bacterial nahG transgene that degrades SA to provide evidence for a Cmv2b-sensitive antiviral defense mechanism in tobacco in which SA acts as a positive modifier but not as an essential component. We propose that SA induces virus resistance by potentiating a RNA-silencing antiviral defense that is targeted by Cmv2b.

Download Full-text