Intraspecies Diversity of the Industrial Yeast StrainsSaccharomyces cerevisiaeandSaccharomyces pastorianusBased on Analysis of the Sequences of the Internal Transcribed Spacer (ITS) Regions and the D1/D2 Region of 26S rDNA

A new polypore, Ceriporia albomellea, collected from tropical China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 µm. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions supported C. albomellea as a distinctive species belonging to Ceriporia.

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Reticulascaceae hyphomycetes from submerged wood in Yunnan, China

Phytotaxa ◽

10.11646/phytotaxa.348.3.2 ◽

2018 ◽

Vol 348 (3) ◽

pp. 187 ◽

Cited By ~ 1

Author(s):

SAJEEWA S.N. MAHARACHCHIKUMBURA ◽

ZONG-LONG LUO ◽

HONG-YAN SU ◽

ABDULLAH M. AL-SADI ◽

RATCHADAWAN CHEEWANGKOON

Keyword(s):

Rna Polymerase Ii ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Yunnan Province ◽

Large Subunit ◽

Protein Subunit ◽

Morphological Examination ◽

Gene Encoding ◽

Its Regions ◽

Taxonomic Novelties

Asexual members of Reticulascaceae (Glomerellales, Hypocreomycetidae) are generally hyphomycetes and usually found as saprobes occurring on various hosts in lentic habitats. A brief survey of the freshwater fungi in Yunnan Province, China, was carried out and four species of Reticulascaceae were isolated. Morphological examination and sequence analysis of large subunit of the nuclear ribosomal RNA (LSU), RNA polymerase II gene encoding the second largest protein subunit (RPB2) and internal transcribed spacer (ITS) regions provide evidence for two taxonomic novelties of Kylindria (K. aquatica and K. chinensis). Two other collections comprised two previously described species of Cylindrotrichum (C. clavatum and C. gorii). Descriptions and illustrations of the above taxa are provided and new species are discussed with comparable taxa.

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Genetic Diversity of Trypanosoma evansi in Buffalo based on Internal Transcribed Spacer (ITS) Regions

Journal of Veterinary Medical Science ◽

10.1292/jvms.69.487 ◽

2007 ◽

Vol 69 (5) ◽

pp. 487-493 ◽

Cited By ~ 12

Author(s):

Sintawee KHUCHAREONTAWORN ◽

Phirom SINGHAPHAN ◽

Nareerat VISESHAKUL ◽

Kosum CHANSIRI

Keyword(s):

Genetic Diversity ◽

Internal Transcribed Spacer ◽

Trypanosoma Evansi ◽

Its Regions

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Is Rhizoplaca (Lecanorales, lichenized Ascomycota) a monophyletic genus?

Canadian Journal of Botany ◽

10.1139/b00-006 ◽

2000 ◽

Vol 78 (3) ◽

pp. 318-327 ◽

Cited By ~ 22

Author(s):

U Arup ◽

M Grube

Keyword(s):

Internal Transcribed Spacer ◽

Type Species ◽

Secondary Chemistry ◽

Its Regions ◽

Large Genus

Rhizoplaca Zopf is a genus characterized by an umbilicate thallus with an upper and a lower cortex, as well as a cupulate hypothecium. It has been considered to be related to Lecanora Ach., the type genus of the Lecanoraceae and, in particular, to the lobate species of this genus. The phylogeny of Rhizoplaca, the monotypic Arctopeltis thuleana Poelt, and a number of representatives of different groups of Lecanora is studied, using sequences from the nuclear ribosomal internal transcribed spacer (ITS) regions. The results suggest an origin for Rhizoplaca species within the large genus Lecanora. A well-supported monophyletic assemblage includes the umbilicate type species Rhizoplaca melanophthalma (DC.) Leuck. & Poelt, the lobate Lecanora novomexicana H. Magn., and five vagrant Rhizoplaca species. Rhizoplaca chrysoleuca (Sm.) Zopf and Rhizoplaca subdicrepans (Nyl.) R. Sant. form a separate well-supported group and Rhizoplaca peltata (Ram.) Leuck. & Poelt is more closely related to Lecanora muralis (Schreb.) Rabenh. Together with data on secondary chemistry, the results show that the umbilicate thallus with a lower and an upper cortex, as well as apothecia with a cupulate hypothecium found in Rhizoplaca and A. thuleana, have developed several times in independant lineages in Lecanora. The thallus morphology in lecanoroid lichens is highly variable and does not necessarily reflect phylogenetic relationships.Key words: Rhizoplaca, Lecanora, Lecanorales, phylogeny, ITS.

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Rhodotorula bloemfonteinensis sp. nov., Rhodotorula eucalyptica sp. nov., Rhodotorula orientis sp. nov. and Rhodotorula pini sp. nov., yeasts isolated from monoterpene-rich environments

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.027011-0 ◽

2011 ◽

Vol 61 (9) ◽

pp. 2320-2327 ◽

Cited By ~ 7

Author(s):

Carolina H. Pohl ◽

Martha S. Smit ◽

Jacobus Albertyn

Keyword(s):

Internal Transcribed Spacer ◽

Type Strain ◽

Yeast Species ◽

Novel Species ◽

Carbon Sources ◽

The Other ◽

26S Rdna ◽

Rdna Sequencing ◽

Physiological Tests

Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown Rhodotorula species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499T and CBS 10736) were identical and were closely related to Rhodotorula cycloclastica, a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from R. cycloclastica by standard physiological tests. The other three isolates represent three novel Rhodotorula species, closely related to Sporobolomyces magnisporus. These three species could also be distinguished from other Rhodotorula species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names Rhodotorula eucalyptica sp. nov. (type strain CBS 8499T = NRRL Y-48408T), Rhodotorula pini sp. nov. (type strain CBS 10735T = NRRL Y-48410T), Rhodotorula bloemfonteinensis sp. nov. (type strain CBS 8598T = NRRL Y-48407T) and Rhodotorula orientis sp. nov. (type strain CBS 8594T = NRRL Y-48719T) are proposed. R. eucalyptica and R. pini can also utilize limonene.

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The Importance of Genomics for Deciphering the Invasion Success of the Seagrass Halophila stipulacea in the Changing Mediterranean Sea

Diversity ◽

10.3390/d12070263 ◽

2020 ◽

Vol 12 (7) ◽

pp. 263

Author(s):

Alexandros Tsakogiannis ◽

Tereza Manousaki ◽

Vasileia Anagnostopoulou ◽

Melanthia Stavroulaki ◽

Eugenia T. Apostolaki

Keyword(s):

Mediterranean Sea ◽

Genetic Information ◽

Internal Transcribed Spacer ◽

Eastern Mediterranean ◽

Invasion Success ◽

Sequencing Technology ◽

Mediterranean Populations ◽

Focal Species ◽

Halophila Stipulacea ◽

Its Regions

The Mediterranean Sea is subject to pressures from biological invasion due to coastal anthropic activities and global warming, which potentially modify its biogeography. The Red Sea tropical seagrass Halophila stipulacea entered the Eastern Mediterranean over a century ago, and its occurrence is expanding towards the northwest. Here, we highlight the importance of genomics for deciphering the evolutionary and ecological procedures taking place during the invasion process of H. stipulacea and review the relatively sparse genetic information available for the species to date. We report the first draft whole-genome sequencing of a H. stipulacea individual from Greece, based on Illumina Sequencing technology. A comparison of the Internal Transcribed Spacer (ITS) regions revealed a high divergence of the herein sequenced individual compared to Mediterranean populations sequenced two decades ago, rendering further questions on the evolutionary processes taking place during H. stipulacea adaptation in the invaded Mediterranean Sea. Our work sets the baseline for a future analysis of the invasion genomic of the focal species.

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Allium dönmezii, a new species of Allium sect. Melanocrommyum (Amaryllidaceae) from Turkey: morphological and molecular evidence

Phytotaxa ◽

10.11646/phytotaxa.411.3.3 ◽

2019 ◽

Vol 411 (3) ◽

pp. 194-204

Author(s):

ŞÜKRÜ KARAKUŞ ◽

BİROL MUTLU

Keyword(s):

New Species ◽

Molecular Marker ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Nuclear Ribosomal Dna ◽

Molecular Evidence ◽

Its Regions ◽

First Time ◽

East Anatolia ◽

A New Species

Allium dönmezii is described as a new species from east Anatolia, Turkey. The molecular marker ITS (Internal Transcribed Spacer of nuclear ribosomal DNA) confirms that the new species belongs to A. sect. Melanocrommyum. The diagnostic characters of the new species are morphologically compared with the related A. woronowii, A. serpentinicum, and A. moderense, as well as with A. rothii and A. chrysantherum. In addition, the ITS regions of A. purpureoviride and A. chrysantherum were sequenced and uploaded on GenBank for the first time.

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Cryptococcus festucosus sp. nov. a new hymenomycetous yeast in the Holtermannia clade

Canadian Journal of Microbiology ◽

10.1139/w04-099 ◽

2004 ◽

Vol 50 (12) ◽

pp. 1001-1006 ◽

Cited By ~ 7

Author(s):

Wladyslav I Golubev ◽

José P Sampaio ◽

Leonor Alves ◽

Nikita W Golubev

Keyword(s):

Internal Transcribed Spacer ◽

Type Strain ◽

Novel Species ◽

Biosphere Reserve ◽

Sequence Analyses ◽

Internal Transcribed Spacer Region ◽

26S Rdna ◽

Yeast Strains ◽

Rdna Sequencing ◽

Steppe Plants

Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains and strain CBS 8016 have almost identical sequences and belong to the Holtermannia clade of the Tremellomycetidae (Basidiomycota, Hymenomycetes). A novel species named Cryptococcus festucosus (type strain VKM Y-2930) is proposed to accommodate these strains. Physiological characteristics and mycocin sensitivity patterns distinguishing Cryptococcus festucosus from the other species of this clade are presented.Key words: mycocin sensitivity, rDNA sequencing, Cryptococcus, Holtermannia.

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